| COUFALÍKOVÁ, Kateřina, Iva BENEŠOVÁ, Tomáš VACULOVIČ, Viktor KANICKÝ and Jan PREISLER. LC coupled to ESI, MALDI and ICP MS - A multiple hyphenation for metalloproteomic studies. Analytica Chimica Acta. Amsterdam: Elsevier Science BV, 2017, vol. 968, May, p. 58-65. ISSN 0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2017.03.016. |
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@article{1386370,
author = {Coufalíková, Kateřina and Benešová, Iva and Vaculovič, Tomáš and Kanický, Viktor and Preisler, Jan},
article_location = {Amsterdam},
article_number = {May},
doi = {http://dx.doi.org/10.1016/j.aca.2017.03.016},
keywords = {Matrix-assisted laser desorption/ionization; mass spectrometry; Inductively coupled plasma mass; spectrometry; Electrospray ionization mass spectrometry; Liquid chromatography; Metallothionein isoforms; Metal complexes},
language = {eng},
issn = {0003-2670},
journal = {Analytica Chimica Acta},
title = {LC coupled to ESI, MALDI and ICP MS - A multiple hyphenation for metalloproteomic studies},
volume = {968},
year = {2017}
}
TY - JOUR
ID - 1386370
AU - Coufalíková, Kateřina - Benešová, Iva - Vaculovič, Tomáš - Kanický, Viktor - Preisler, Jan
PY - 2017
TI - LC coupled to ESI, MALDI and ICP MS - A multiple hyphenation for metalloproteomic studies
JF - Analytica Chimica Acta
VL - 968
IS - May
SP - 58-65
EP - 58-65
PB - Elsevier Science BV
SN - 00032670
KW - Matrix-assisted laser desorption/ionization
KW - mass spectrometry
KW - Inductively coupled plasma mass
KW - spectrometry
KW - Electrospray ionization mass spectrometry
KW - Liquid chromatography
KW - Metallothionein isoforms
KW - Metal complexes
N2 - A new multiple detection arrangement for liquid chromatography (LC) that supplements conventional electrospray ionization (ESI) mass spectrometry (MS) detection with two complementary detection techniques, matrix-assisted laser desorption/ionization (MALDI) MS and substrate-assisted laser desorption inductively coupled plasma (SALD ICP) MS has been developed. The combination of the molecular and elemental detectors in a single separation run is accomplished by utilizing a commercial MALDI target made of conductive plastic. The proposed platform provides a number of benefits in today's metalloproteomic applications, which are demonstrated by analysis of a metallothionein mixture. To maintain metallothionein complexes, separation is carried out at a neutral pH. The effluent is split; a major portion is directed to ESI MS while the remaining 1.8% fraction is deposited onto a plastic MALDI target. Dried droplets are overlaid with MALDI matrix and analysed consecutively by MALDI MS and SALD ICP MS. In the ESI MS spectra, the MT isoform complexes with metals and their stoichiometry are determined; the apoforms are revealed in the MALDI MS spectra. Quantitative determination of metallothionein isoforms is performed via determination of metals in the complexes of the individual protein isoforms using SALD ICP MS. (C) 2017 Elsevier B.V. All rights reserved.
ER -
COUFALÍKOVÁ, Kateřina, Iva BENEŠOVÁ, Tomáš VACULOVIČ, Viktor KANICKÝ and Jan PREISLER. LC coupled to ESI, MALDI and ICP MS - A multiple hyphenation for metalloproteomic studies. \textit{Analytica Chimica Acta}. Amsterdam: Elsevier Science BV, 2017, vol.~968, May, p.~58-65. ISSN~0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2017.03.016.
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