Detailed Information on Publication Record
2017
Expression of Genes Encoding Zona Pellucida Glycoproteins and Cortical Granule Distribution in Porcine Oocytes Isolated from Small and Medium Follicles in Relation to Puberty Status of Donors
JEŠETA, Michal, Joanna BUDNA, Wieslawa KRANC, Sarka HANULAKOVA, Artur BRYJA et. al.Basic information
Original name
Expression of Genes Encoding Zona Pellucida Glycoproteins and Cortical Granule Distribution in Porcine Oocytes Isolated from Small and Medium Follicles in Relation to Puberty Status of Donors
Authors
JEŠETA, Michal (203 Czech Republic, guarantor, belonging to the institution), Joanna BUDNA (616 Poland), Wieslawa KRANC (616 Poland), Sarka HANULAKOVA (203 Czech Republic), Artur BRYJA (616 Poland), Adrian CHACHULA (616 Poland), Sylwia CIESIÓLKA (616 Poland), Katarzyna WOJTANOWICZ-MARKIEWICZ (616 Poland), Dorota BUKOWSKA (616 Poland), Klaus-Peter BRUSSOW (616 Poland), Malgorzata BRUSKA (616 Poland), Michal NOWICKI (616 Poland), Pawel ANTOSIK (616 Poland), Pavlina HULINSKA (203 Czech Republic), Marie MACHATKOVA (203 Czech Republic), Maciej ZABEL (616 Poland) and Bartosz KEMPISTY (616 Poland)
Edition
Czech Journal of Animal Science, Prague, Czech Academy Agricultural Sciences, 2017, 1212-1819
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
40101 Agriculture
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 0.955
RIV identification code
RIV/00216224:14110/17:00097251
Organization unit
Faculty of Medicine
UT WoS
000403547200002
Keywords in English
pig; ovarian cycle; maturation; developmental competence; folliculogenesis
Tags
Tags
International impact, Reviewed
Změněno: 20/3/2018 18:30, Soňa Böhmová
Abstract
V originále
The zona pellucida proteins belong to a group of proteins that regulate the processes of gamete recognition, interaction, and fusion, since they are recognized as primary and secondary sperm receptors. It is suggested that cortical granule distribution is significantly associated with the zona pellucida structure and membrane preparation to cortical and acrosome reaction. Therefore, this study investigated the zona pellucida marker gene expression (ZP2 and ZP4 protein) in relation to cortical granules distribution in oocytes and puberty status of donors. Oocytes were collected from adult cyclic sows (isolated from medium and small follicles) and juvenile gilts (isolated only from small follicles). The oocytes were examined by RT-qPCR and by confocal microscopy. The expression of genes for ZP2 and ZP4 protein in oocytes collected from small follicles from cycling sows was higher in comparison to oocytes from medium follicles or oocytes collected from small follicles from juvenile gilts (P < 0.001). We also observed increased expression of both ZP2 and ZP4 mRNA in oocytes collected from small follicles (juvenile gilts) as compared to medium follicles (cycling sows) (P < 0.001). Moreover, we found a difference in the distribution of cortical granules. In oocytes from medium follicles, the peripheral localization of cortical granules was twice higher than their central concentration. However, in oocytes derived from small follicles (cyclic sows) cortical granules in comparison to oocytes from medium follicles were more centrally localized (P < 0.05). It has been suggested that the donor puberty status and the size of follicles significantly influenced the zona pellucida gene expression and cortical granule localization in porcine oocytes. This is accompanied by fertilization specificity and fertilizability of porcine oocytes in vitro.
Links
| ROZV/24/LF/2016, interní kód MU |
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