Cytoskeletal elements and motility in the archigregarine
Selenidium sp.: observations on native and drug-treated
parasites

a 2017

Cytoskeletal elements and motility in the archigregarine Selenidium sp.: observations on native and drug-treated parasites

KOVÁČIKOVÁ, Magdaléna, Gita PASKEROVA G., Andrei DIAKIN and Andrea BARDŮNEK VALIGUROVÁ

Basic information

Original name

Cytoskeletal elements and motility in the archigregarine Selenidium sp.: observations on native and drug-treated parasites

Authors

KOVÁČIKOVÁ, Magdaléna (703 Slovakia, guarantor, belonging to the institution), Gita PASKEROVA G. (643 Russian Federation), Andrei DIAKIN (643 Russian Federation, belonging to the institution) and Andrea BARDŮNEK VALIGUROVÁ (703 Slovakia, belonging to the institution)

Edition

15th International Congress of Protistology (ICOP), 2017

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/17:00094872

Organization unit

Faculty of Science

Keywords in English

Apicomplexa; gregarine; motility; actin; alpha-tubulin

Abstract

V originále

Ancestral apicomplexan lineages, including gregarines, represent organisms suitable for research on motility evolution and diversification. Archigregarines are restricted to a marine environment and, based on their plesiomorfic characters, represent basal group of gregarines and perhaps apicomplexans as whole. The vermiform trophozoites of Selenidium archigregarines possess apical complex and exhibit pendular, rolling or coiling movement. The presented study revealed the fundamental role of cytoskeletal proteins, such as actin and alpha-tubulin, in archigregarines motility, and allowed us to compare the mechanism of their movement to the gliding machinery (= the so called glideosome concept) described in apicomplexan zoites. Motility of Selenidium sp. parasitising the intestine of polychaete Pygospio elegans (Spionidae) was investigated using probes influencing polymerisation of cytoskeletal proteins. To verify the role of actin filaments, jasplakinolide (induces actin polymerisation) and cytochalasin D (blocks polymerisation of actin microfilaments) were applied. The effect of disruption of subpellicular microtubules on motility was monitored after treatment with oryzalin and colchicine (both probes destroy existing microtubules and inhibit tubulin polymerization). Despite we succeed to completely block the motility of archigregarines, our experiments revealed the tolerance of organisms to cytoskeletal drugs’ concentrations routinely used in other apicomplexans and their prolonged survival in extreme conditions. Evaluation of Selenidium sp. surface observation did not showed any damages or abnormalities of pellicle after drugs’ applications. Though, evident changes in distribution of cytoskeletal proteins after confocal laser scanning microscopic analysis were revealed. Oryzalin and colchicine showed the highest impact on archigregarine movement.

Links

GBP505/12/G112, research and development project

Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

Citovat

KOVÁČIKOVÁ, Magdaléna, Gita PASKEROVA G., Andrei DIAKIN and Andrea BARDŮNEK VALIGUROVÁ. Cytoskeletal elements and motility in the archigregarine Selenidium sp.: observations on native and drug-treated parasites. In 15th International Congress of Protistology (ICOP). 2017.

@proceedings{1386697,
   author = {Kováčiková, Magdaléna and Paskerova G., Gita and Diakin, Andrei and Bardůnek Valigurová, Andrea},
   booktitle = {15th International Congress of Protistology (ICOP)},
   keywords = {Apicomplexa; gregarine; motility; actin; alpha-tubulin},
   language = {eng},
   title = {Cytoskeletal elements and motility in the archigregarine Selenidium sp.: observations on native and drug-treated parasites},
   year = {2017}
}

TY  - CONF
ID  - 1386697
AU  - Kováčiková, Magdaléna - Paskerova G., Gita - Diakin, Andrei - Bardůnek Valigurová, Andrea
PY  - 2017
TI  - Cytoskeletal elements and motility in the archigregarine Selenidium sp.: observations on native and drug-treated parasites
KW  - Apicomplexa
KW  - gregarine
KW  - motility
KW  - actin
KW  - alpha-tubulin
N2  - Ancestral apicomplexan lineages, including gregarines, represent organisms suitable for research on motility evolution and diversification. Archigregarines are restricted to a marine environment and, based on their plesiomorfic characters, represent basal group of gregarines and perhaps apicomplexans as whole. The vermiform trophozoites of Selenidium archigregarines possess apical complex and exhibit pendular, rolling or coiling movement. The presented study revealed the fundamental role of cytoskeletal proteins, such as actin and alpha-tubulin, in archigregarines motility, and allowed us to compare the mechanism of their movement to the gliding machinery (= the so called glideosome concept) described in apicomplexan zoites. Motility of Selenidium sp. parasitising the intestine of polychaete Pygospio elegans (Spionidae) was investigated using probes influencing polymerisation of cytoskeletal proteins. To verify the role of actin filaments, jasplakinolide (induces actin polymerisation) and cytochalasin D (blocks polymerisation of actin microfilaments) were applied. The effect of disruption of subpellicular microtubules on motility was monitored after treatment with oryzalin and colchicine (both probes destroy existing microtubules and inhibit tubulin polymerization). Despite we succeed to completely block the motility of archigregarines, our experiments revealed the tolerance of organisms to cytoskeletal drugs’ concentrations routinely used in other apicomplexans and their prolonged survival in extreme conditions. Evaluation of Selenidium sp. surface observation did not showed any damages or abnormalities of pellicle after drugs’ applications. Though, evident changes in distribution of cytoskeletal proteins after confocal laser scanning microscopic analysis were revealed. Oryzalin and colchicine showed the highest impact on archigregarine movement.
ER  -

KOVÁČIKOVÁ, Magdaléna, Gita PASKEROVA G., Andrei DIAKIN and Andrea BARDŮNEK VALIGUROVÁ. Cytoskeletal elements and motility in the archigregarine Selenidium sp.: observations on native and drug-treated parasites. In \textit{15th International Congress of Protistology (ICOP)}. 2017.

Detailed Information on Publication Record