Ultrastructural aspects of surface and attachment clamps of
Paradiplozoon homoion (Monogena, Diplozoidae)

a 2017

Ultrastructural aspects of surface and attachment clamps of Paradiplozoon homoion (Monogena, Diplozoidae)

KONSTANZOVÁ, Veronika, Božena KOUBKOVÁ and Milan GELNAR

Basic information

Original name

Ultrastructural aspects of surface and attachment clamps of Paradiplozoon homoion (Monogena, Diplozoidae)

Authors

KONSTANZOVÁ, Veronika (203 Czech Republic, belonging to the institution), Božena KOUBKOVÁ (203 Czech Republic, belonging to the institution) and Milan GELNAR (203 Czech Republic, guarantor, belonging to the institution)

Edition

8th International Symposium on Monogenea, 2017

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/17:00094873

Organization unit

Faculty of Science

ISBN

978-80-210-8665-4

Keywords in English

Paradiplozoon homoion

Abstract

V originále

Paradiplozoon homoion (Bychowsky et Nagibina, 1959) is a representative of blood-feeding ectoparasites from family Diplozoidae with specific life-cycle, parasitizing mainly on gills of cyprinid fish, where they can cause mechanical damage to the gill filaments, initiating the development of secondary infections (bacterial, mycotic) and anemia. During the diplozoon life cycle two larvae, the diporpae, pair and subsequently fuse permanently producing the typical X-shaped diplozoon body arrangement, this is a unique character for Diplozoidae. The samples of P. homoion were collected from the gills of bleak (Alburnus alburnus), which were caught in the littoral zone of the Mušov lowland reservoir. The fish gills were extracted and checked for the presence of all diplozoid ontogenetic stages. Parasites intended for transmission electron microscopy (TEM) were washed in freshwater to remove any remaining mucus and then fixed in 2% osmium tetroxide and dehydrated through an ascending acetone series. The dehydrated samples were embedded in Spurr resin. In order to obtain a better orientation in basic diplozoon morphology several semi-thin sections (0.5 µm) of whole worm bodies were cut using a Leica EM UC6i. Sections were stained with toluidine blue. Ultrathin sections were contrasted with uranyl acetate, followed by citrate, and examined using a JEOL JEM-1010 TEM. The presentation provides detailed ultrastructural data on the surface and haptoral attachment clamps of P. homoion and brings new insights into the ultrastructure of Diplozoidae. Our findings support the hypothesis that the structure of particular neodermal compartments is similar within the Platyhelminthes.

Links

GBP505/12/G112, research and development project

Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

MSM0021622416, plan (intention)

Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time

Citovat

KONSTANZOVÁ, Veronika, Božena KOUBKOVÁ and Milan GELNAR. Ultrastructural aspects of surface and attachment clamps of Paradiplozoon homoion (Monogena, Diplozoidae). In 8th International Symposium on Monogenea. 2017. ISBN 978-80-210-8665-4.

@proceedings{1386730,
   author = {Konstanzová, Veronika and Koubková, Božena and Gelnar, Milan},
   booktitle = {8th International Symposium on Monogenea},
   keywords = {Paradiplozoon homoion},
   language = {eng},
   isbn = {978-80-210-8665-4},
   title = {Ultrastructural aspects of surface and attachment clamps of Paradiplozoon homoion (Monogena, Diplozoidae)},
   year = {2017}
}

TY  - CONF
ID  - 1386730
AU  - Konstanzová, Veronika - Koubková, Božena - Gelnar, Milan
PY  - 2017
TI  - Ultrastructural aspects of surface and attachment clamps of Paradiplozoon homoion (Monogena, Diplozoidae)
SN  - 9788021086654
KW  - Paradiplozoon homoion
N2  - Paradiplozoon homoion (Bychowsky et Nagibina, 1959) is a representative of blood-feeding ectoparasites from family Diplozoidae with specific life-cycle, parasitizing mainly on gills of cyprinid fish, where they can cause mechanical damage to the gill filaments, initiating the development of secondary infections (bacterial, mycotic) and anemia. During the diplozoon life cycle two larvae, the diporpae, pair and subsequently fuse permanently producing the typical X-shaped diplozoon body arrangement, this is a unique character for Diplozoidae. The samples of P. homoion were collected from the gills of bleak (Alburnus alburnus), which were caught in the littoral zone of the Mušov lowland reservoir. The fish gills were extracted and checked for the presence of all diplozoid ontogenetic stages. Parasites intended for transmission electron microscopy (TEM) were washed in freshwater to remove any remaining mucus and then fixed in 2% osmium tetroxide and dehydrated through an ascending acetone series. The dehydrated samples were embedded in Spurr resin. In order to obtain a better orientation in basic diplozoon morphology several semi-thin sections (0.5 µm) of whole worm bodies were cut using a Leica EM UC6i. Sections were stained with toluidine blue. Ultrathin sections were contrasted with uranyl acetate, followed by citrate, and examined using a JEOL JEM-1010 TEM. The presentation provides detailed ultrastructural data on the surface and haptoral attachment clamps of P. homoion and brings new insights into the ultrastructure of Diplozoidae. Our findings support the hypothesis that the structure of particular neodermal compartments is similar within the Platyhelminthes.
ER  -

KONSTANZOVÁ, Veronika, Božena KOUBKOVÁ and Milan GELNAR. Ultrastructural aspects of surface and attachment clamps of Paradiplozoon homoion (Monogena, Diplozoidae). In \textit{8th International Symposium on Monogenea}. 2017. ISBN~978-80-210-8665-4.

Detailed Information on Publication Record