Microscopic techniques in the study of African parasitic
nematodes

a 2017

Microscopic techniques in the study of African parasitic nematodes

HODOVÁ, Eliška, Šárka MAŠOVÁ, Kristýna ROSÍKOVÁ, Maarten Pieterjan VANHOVE, Iveta HODOVÁ et. al.

Basic information

Original name

Microscopic techniques in the study of African parasitic nematodes

Authors

HODOVÁ, Eliška (203 Czech Republic, belonging to the institution), Šárka MAŠOVÁ (203 Czech Republic, belonging to the institution), Kristýna ROSÍKOVÁ (203 Czech Republic), Maarten Pieterjan VANHOVE (56 Belgium, belonging to the institution) and Iveta HODOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Mikroskopie 2017, 2017

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Slovakia

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/17:00094883

Organization unit

Faculty of Science

Keywords in English

Nematodes; Scanning electron microscopy; Lake Tanganyika

Změněno: 14/8/2017 10:00, Mgr. Iveta Hodová, Ph.D.

Abstract

V originále

Based on light and scanning electron microscopic studies, nematodes of the subgenus Procamallanus (Spirocamallanus) have been recorded in the intestine of six host cichlid species (Cyprichromis microlepidotus, Boulengerochromis microlepsis, Eretmodus marksmithi, Neolamprologus mondabu, Bathybates graueri, Oreochromis niloticus, Trematocara unimaculatum) were examined. Hosts were collected during a survey of fish parasites in the Lake Tanganyika (lakeside of Burundi). Obtained nematodes (glycerine-cleared) were examined by the Olympus BX51 light microscope (LM) equipped with differential interference contrast. They were measured and documented (drawings and micrographs) with all important signs, like buccal capsule, nerve ring, excretory pore, papillae, deirids, tail; males spiculae and females vulva. Scanning electron microscopy (SEM) was used for study of surface structures (cuticle ornamentation, number and distribution of papillae, deirids, amphids), for interior view to the capsule with ornamentation and for protruded males spiculae. The samples were examined using TESCAN MIRA3 FEG-SEM at an accelerating voltage of 5 kV. Based on our obtained results from LM and SEM we assume the presence of new species. Obtained results will be part of a High School Scientific Activity (SOČ) of Eliška Hodová (Gymnasium Brno – Řečkovice) “Parasitic nematodes from cichlids of the Lake Tanganyika“.

Links

GBP505/12/G112, research and development project

Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

Citovat

HODOVÁ, Eliška, Šárka MAŠOVÁ, Kristýna ROSÍKOVÁ, Maarten Pieterjan VANHOVE and Iveta HODOVÁ. Microscopic techniques in the study of African parasitic nematodes. In Mikroskopie 2017. 2017.

@proceedings{1387264,
   author = {Hodová, Eliška and Mašová, Šárka and Rosíková, Kristýna and Vanhove, Maarten Pieterjan and Hodová, Iveta},
   booktitle = {Mikroskopie 2017},
   keywords = {Nematodes; Scanning electron microscopy; Lake Tanganyika},
   language = {eng},
   title = {Microscopic techniques in the study of African parasitic nematodes},
   year = {2017}
}

TY  - CONF
ID  - 1387264
AU  - Hodová, Eliška - Mašová, Šárka - Rosíková, Kristýna - Vanhove, Maarten Pieterjan - Hodová, Iveta
PY  - 2017
TI  - Microscopic techniques in the study of African parasitic nematodes
KW  - Nematodes
KW  - Scanning electron microscopy
KW  - Lake Tanganyika
N2  - Based on light and scanning electron microscopic studies, nematodes of the subgenus Procamallanus (Spirocamallanus) have been recorded in the intestine of six host cichlid species (Cyprichromis microlepidotus, Boulengerochromis microlepsis, Eretmodus marksmithi, Neolamprologus mondabu, Bathybates graueri, Oreochromis niloticus, Trematocara unimaculatum) were examined. Hosts were collected during a survey of fish parasites in the Lake Tanganyika (lakeside of Burundi). Obtained nematodes (glycerine-cleared) were examined by the Olympus BX51 light microscope (LM) equipped with differential interference contrast. They were measured and documented (drawings and micrographs) with all important signs, like buccal capsule, nerve ring, excretory pore, papillae, deirids, tail; males spiculae and females vulva. Scanning electron microscopy (SEM) was used for study of surface structures (cuticle ornamentation, number and distribution of papillae, deirids, amphids), for interior view to the capsule with ornamentation and for protruded males spiculae. The samples were examined using TESCAN MIRA3 FEG-SEM at an accelerating voltage of 5 kV. Based on our obtained results from LM and SEM we assume the presence of new species. Obtained results will be part of a High School Scientific Activity (SOČ) of Eliška Hodová (Gymnasium Brno – Řečkovice) “Parasitic nematodes from cichlids of the Lake Tanganyika“.
ER  -

HODOVÁ, Eliška, Šárka MAŠOVÁ, Kristýna ROSÍKOVÁ, Maarten Pieterjan VANHOVE and Iveta HODOVÁ. Microscopic techniques in the study of African parasitic nematodes. In \textit{Mikroskopie 2017}. 2017.

Detailed Information on Publication Record