Microscopic anatomy of monogenean parasite Paradiplozoon
homoion

a 2017

Microscopic anatomy of monogenean parasite Paradiplozoon homoion

HODOVÁ, Iveta, Radim SONNEK, Milan GELNAR and Andrea BARDŮNEK VALIGUROVÁ

Basic information

Original name

Microscopic anatomy of monogenean parasite Paradiplozoon homoion

Authors

HODOVÁ, Iveta (203 Czech Republic, guarantor, belonging to the institution), Radim SONNEK (203 Czech Republic), Milan GELNAR (203 Czech Republic, belonging to the institution) and Andrea BARDŮNEK VALIGUROVÁ (703 Slovakia, belonging to the institution)

Edition

8th International Symposium on Monogenea, 2017

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/17:00094884

Organization unit

Faculty of Science

ISBN

978-80-210-8665-4

Keywords in English

Diplozoidae; confocal microscopy; electron microscopy; anatomy

Abstract

V originále

The species Paradiplozoon homoion (Bychowsky et Nagibina, 1959) belongs to the family Diplozoidae (Monogenea), representing blood-feeding gill ectoparasites of freshwater fish. Diplozoids exhibit extraordinary morphology and life cycle involving permanent fusion of two larval worms and their subsequent transformation into one individual. Samples of P. homoion were collected from the gills of roach (Rutilus rutilus L.), bleak (Alburnus alburnus L.) and gudgeon (Gobio gobio L.) caught by electrofishing or seine netting in the Mušov reservoir (southern Moravia, Czech Republic). Using a combined approach of confocal and electron microscopy, adults of monogenean parasite P. homoion were investigated for the body organisation and adaptation to the ectoparasitic lifestyle, with emphasis on worm’s forebody and hindbody. Special attention was given to the parasite’s tegument, digestive, reproductive, and excretory systems. Labelling of filamentous actin with fluorescent phalloidin revealed the musculature of the body wall, buccal suckers, the pharynx as well as some parts of reproductive tract. Staining with chloride carmine and counterstaining with DAPI proved to be powerful tools for confocal microscopic detection of various cell types including putative gland cells. Immunofluorescent detection of alfa-tubulin showed to be suitable for visualisation of excretory system and some parts of nervous system.

Links

GBP505/12/G112, research and development project

Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

Citovat

HODOVÁ, Iveta, Radim SONNEK, Milan GELNAR and Andrea BARDŮNEK VALIGUROVÁ. Microscopic anatomy of monogenean parasite Paradiplozoon homoion. In 8th International Symposium on Monogenea. 2017. ISBN 978-80-210-8665-4.

@proceedings{1387266,
   author = {Hodová, Iveta and Sonnek, Radim and Gelnar, Milan and Bardůnek Valigurová, Andrea},
   booktitle = {8th International Symposium on Monogenea},
   keywords = {Diplozoidae; confocal microscopy; electron microscopy; anatomy},
   language = {eng},
   isbn = {978-80-210-8665-4},
   title = {Microscopic anatomy of monogenean parasite Paradiplozoon homoion},
   year = {2017}
}

TY  - CONF
ID  - 1387266
AU  - Hodová, Iveta - Sonnek, Radim - Gelnar, Milan - Bardůnek Valigurová, Andrea
PY  - 2017
TI  - Microscopic anatomy of monogenean parasite Paradiplozoon homoion
SN  - 9788021086654
KW  - Diplozoidae
KW  - confocal microscopy
KW  - electron microscopy
KW  - anatomy
N2  - The species Paradiplozoon homoion (Bychowsky et Nagibina, 1959) belongs to the family Diplozoidae (Monogenea), representing blood-feeding gill ectoparasites of freshwater fish. Diplozoids exhibit extraordinary morphology and life cycle involving permanent fusion of two larval worms and their subsequent transformation into one individual. Samples of P. homoion were collected from the gills of roach (Rutilus rutilus L.), bleak (Alburnus alburnus L.) and gudgeon (Gobio gobio L.) caught by electrofishing or seine netting in the Mušov reservoir (southern Moravia, Czech Republic). Using a combined approach of confocal and electron microscopy, adults of monogenean parasite P. homoion were investigated for the body organisation and adaptation to the ectoparasitic lifestyle, with emphasis on worm’s forebody and hindbody. Special attention was given to the parasite’s tegument, digestive, reproductive, and excretory systems. Labelling of filamentous actin with fluorescent phalloidin revealed the musculature of the body wall, buccal suckers, the pharynx as well as some parts of reproductive tract. Staining with chloride carmine and counterstaining with DAPI proved to be powerful tools for confocal microscopic detection of various cell types including putative gland cells. Immunofluorescent detection of alfa-tubulin showed to be suitable for visualisation of excretory system and some parts of nervous system.
ER  -

HODOVÁ, Iveta, Radim SONNEK, Milan GELNAR and Andrea BARDŮNEK VALIGUROVÁ. Microscopic anatomy of monogenean parasite Paradiplozoon homoion. In \textit{8th International Symposium on Monogenea}. 2017. ISBN~978-80-210-8665-4.

Detailed Information on Publication Record