White-nose syndrome pathology grading in Nearctic and
Palearctic bats

J 2017

White-nose syndrome pathology grading in Nearctic and Palearctic bats

PIKULA, Jiri, Sybill AMELON, Hana BANDOUCHOVA, Tomáš BARTONIČKA, Hana BERKOVÁ et. al.

Basic information

Original name

White-nose syndrome pathology grading in Nearctic and Palearctic bats

Authors

PIKULA, Jiri (203 Czech Republic), Sybill AMELON (840 United States of America), Hana BANDOUCHOVA (203 Czech Republic), Tomáš BARTONIČKA (203 Czech Republic, guarantor, belonging to the institution), Hana BERKOVÁ (203 Czech Republic), Jiri BRICHTA (203 Czech Republic), Sarah HOOPER (840 United States of America), Tomasz KOKUREWICZ (616 Poland), Miroslav KOLARIK (203 Czech Republic), Bernd KÖLLNER (276 Germany), Veronika KOVACOVA (203 Czech Republic), Petr LINHART (203 Czech Republic), Vladimir PIACEK (203 Czech Republic), Gregory TURNER (840 United States of America), Jan ZUKAL (203 Czech Republic, belonging to the institution) and Natália MARTÍNKOVÁ (203 Czech Republic, belonging to the institution)

Edition

Plos One, SAN FRANCISCO, PUBLIC LIBRARY SCIENCE, 2017, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.766

RIV identification code

RIV/00216224:14310/17:00097333

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1371/journal.pone.0180435

UT WoS

000406768200008

Keywords in English

severity; white-nose syndrom;bats; semi-quantitative pathology

Abstract

V originále

While white-nose syndrome (WNS) has decimated hibernating bat populations in the Nearc- tic, species from the Palearctic appear to cope better with the fungal skin infection causing WNS. This has encouraged multiple hypotheses on the mechanisms leading to differential survival of species exposed to the same pathogen. To facilitate intercontinental compari- sons, we proposed a novel pathogenesis-based grading scheme consistent with WNS diag- nosis histopathology criteria. UV light-guided collection was used to obtain single biopsies from Nearctic and Palearctic bat wing membranes non-lethally. The proposed scheme scores eleven grades associated with WNS on histopathology. Given weights reflective of grade severity, the sum of findings from an individual results in weighted cumulative WNS pathology score. The probability of finding fungal skin colonisation and single, multiple or confluent cupping erosions increased with increase in Pseudogymnoascus destructans load. Increasing fungal load mimicked progression of skin infection from epidermal surface colonisation to deep dermal invasion. Similarly, the number of UV-fluorescent lesions increased with increasing weighted cumulative WNS pathology score, demonstrating con- gruence between WNS-associated tissue damage and extent of UV fluorescence. In a case report, we demonstrated that UV-fluorescence disappears within two weeks of euthermy. Change in fluorescence was coupled with a reduction in weighted cumulative WNS pathol- ogy score, whereby both methods lost diagnostic utility. While weighted cumulative WNS pathology scores were greater in the Nearctic than Palearctic, values for Nearctic bats were within the range of those for Palearctic species. Accumulation of wing damage probably influences mortality in affected bats, as demonstrated by a fatal case of Myotis daubentonii with natural WNS infection and healing in Myotis myotis. The proposed semi-quantitative pathology score provided good agreement between experienced raters, showing it to be a powerful and widely applicable tool for defining WNS severity.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

PIKULA, Jiri, Sybill AMELON, Hana BANDOUCHOVA, Tomáš BARTONIČKA, Hana BERKOVÁ, Jiri BRICHTA, Sarah HOOPER, Tomasz KOKUREWICZ, Miroslav KOLARIK, Bernd KÖLLNER, Veronika KOVACOVA, Petr LINHART, Vladimir PIACEK, Gregory TURNER, Jan ZUKAL and Natália MARTÍNKOVÁ. White-nose syndrome pathology grading in Nearctic and Palearctic bats. Plos One. SAN FRANCISCO: PUBLIC LIBRARY SCIENCE, 2017, vol. 12, No 8, p. nestránkováno, 21 pp. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0180435.

@article{1387321,
   author = {Pikula, Jiri and Amelon, Sybill and Bandouchova, Hana and Bartonička, Tomáš and Berková, Hana and Brichta, Jiri and Hooper, Sarah and Kokurewicz, Tomasz and Kolarik, Miroslav and Köllner, Bernd and Kovacova, Veronika and Linhart, Petr and Piacek, Vladimir and Turner, Gregory and Zukal, Jan and Martínková, Natália},
   article_location = {SAN FRANCISCO},
   article_number = {8},
   doi = {http://dx.doi.org/10.1371/journal.pone.0180435},
   keywords = {severity; white-nose syndrom;bats; semi-quantitative pathology},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos One},
   title = {White-nose syndrome pathology grading in Nearctic and Palearctic bats},
   volume = {12},
   year = {2017}
}

TY  - JOUR
ID  - 1387321
AU  - Pikula, Jiri - Amelon, Sybill - Bandouchova, Hana - Bartonička, Tomáš - Berková, Hana - Brichta, Jiri - Hooper, Sarah - Kokurewicz, Tomasz - Kolarik, Miroslav - Köllner, Bernd - Kovacova, Veronika - Linhart, Petr - Piacek, Vladimir - Turner, Gregory - Zukal, Jan - Martínková, Natália
PY  - 2017
TI  - White-nose syndrome pathology grading in Nearctic and Palearctic bats
JF  - Plos One
VL  - 12
IS  - 8
SP  - nestránkováno
EP  - nestránkováno
PB  - PUBLIC LIBRARY SCIENCE
SN  - 19326203
KW  - severity
KW  - white-nose syndrom;bats
KW  - semi-quantitative pathology
N2  - While white-nose syndrome (WNS) has decimated hibernating bat populations in the Nearc- tic, species from the Palearctic appear to cope better with the fungal skin infection causing WNS. This has encouraged multiple hypotheses on the mechanisms leading to differential survival of species exposed to the same pathogen. To facilitate intercontinental compari- sons, we proposed a novel pathogenesis-based grading scheme consistent with WNS diag- nosis histopathology criteria. UV light-guided collection was used to obtain single biopsies from Nearctic and Palearctic bat wing membranes non-lethally. The proposed scheme scores eleven grades associated with WNS on histopathology. Given weights reflective of grade severity, the sum of findings from an individual results in weighted cumulative WNS pathology score. The probability of finding fungal skin colonisation and single, multiple or confluent cupping erosions increased with increase in Pseudogymnoascus destructans load. Increasing fungal load mimicked progression of skin infection from epidermal surface colonisation to deep dermal invasion. Similarly, the number of UV-fluorescent lesions increased with increasing weighted cumulative WNS pathology score, demonstrating con- gruence between WNS-associated tissue damage and extent of UV fluorescence. In a case report, we demonstrated that UV-fluorescence disappears within two weeks of euthermy. Change in fluorescence was coupled with a reduction in weighted cumulative WNS pathol- ogy score, whereby both methods lost diagnostic utility. While weighted cumulative WNS pathology scores were greater in the Nearctic than Palearctic, values for Nearctic bats were within the range of those for Palearctic species. Accumulation of wing damage probably influences mortality in affected bats, as demonstrated by a fatal case of Myotis daubentonii with natural WNS infection and healing in Myotis myotis. The proposed semi-quantitative pathology score provided good agreement between experienced raters, showing it to be a powerful and widely applicable tool for defining WNS severity.
ER  -

PIKULA, Jiri, Sybill AMELON, Hana BANDOUCHOVA, Tomáš BARTONIČKA, Hana BERKOVÁ, Jiri BRICHTA, Sarah HOOPER, Tomasz KOKUREWICZ, Miroslav KOLARIK, Bernd KÖLLNER, Veronika KOVACOVA, Petr LINHART, Vladimir PIACEK, Gregory TURNER, Jan ZUKAL and Natália MARTÍNKOVÁ. White-nose syndrome pathology grading in Nearctic and Palearctic bats. \textit{Plos One}. SAN FRANCISCO: PUBLIC LIBRARY SCIENCE, 2017, vol.~12, No~8, p.~nestránkováno, 21 pp. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0180435.

Detailed Information on Publication Record