Characterization of novel bangle lectin from Photorhabdus
asymbiotica with dual sugar-binding specificity and its effect
on host immunity

J 2017

Characterization of novel bangle lectin from Photorhabdus asymbiotica with dual sugar-binding specificity and its effect on host immunity

JANČAŘÍKOVÁ, Gita, Josef HOUSER, Pavel DOBEŠ, Gabriel DEMO, Pavel HYRŠL et. al.

Basic information

Original name

Characterization of novel bangle lectin from Photorhabdus asymbiotica with dual sugar-binding specificity and its effect on host immunity

Authors

JANČAŘÍKOVÁ, Gita (203 Czech Republic, belonging to the institution), Josef HOUSER (203 Czech Republic, belonging to the institution), Pavel DOBEŠ (203 Czech Republic, belonging to the institution), Gabriel DEMO (703 Slovakia, belonging to the institution), Pavel HYRŠL (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

PLoS Pathogens, San Francisco, Public Library of Science, 2017, 1553-7366

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.158

RIV identification code

RIV/00216224:14310/17:00094886

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1371/journal.ppat.1006564

UT WoS

000408758400037

Keywords in English

Lectins; blood; insects; blood groups; carbohydrates; immune response; sedimenation; nematode infections

Abstract

V originále

Photorhabdus asymbiotica is one of the three recognized species of the Photorhabdus genus, which consists of gram-negative bioluminescent bacteria belonging to the family Morganellaceae. These bacteria live in a symbiotic relationship with nematodes from the genus Heterorhabditis, together forming a complex that is highly pathogenic for insects. Unlike other Photorhabdus species, which are strictly entomopathogenic, P. asymbiotica is unique in its ability to act as an emerging human pathogen. Analysis of the P. asymbiotica genome identified a novel fucose-binding lectin designated PHL with a strong sequence similarity to the recently described P. luminescens lectin PLL. Recombinant PHL exhibited high affinity for fucosylated carbohydrates and the unusual disaccharide 3,6-O-Me2-GlcBeta1± 4(2,3-O-Me2)RhaAlpha-O-(p-C6H4)-OCH2CH2NH2 from Mycobacterium leprae. Based on its crystal structure, PHL forms a seven-bladed beta-propeller assembling into a homo-dimer with an inter-subunit disulfide bridge. Investigating complexes with different ligands revealed the existence of two sets of binding sites per monomer - the first type prefers L-fucose and its derivatives, whereas the second type can bind D-galactose. Based on the sequence analysis, PHL could contain up to twelve binding sites per monomer. PHL was shown to interact with all types of red blood cells and insect haemocytes. Interestingly, PHL inhibited the production of reactive oxygen species induced by zymosan A in human blood and antimicrobial activity both in human blood, serum and insect haemolymph. Concurrently, PHL increased the constitutive level of oxidants in the blood and induced melanisation in haemolymph. Our results suggest that PHL might play a crucial role in the interaction of P. asymbiotica with both human and insect hosts.

Links

GA13-25401S, research and development project

Name: Studium proteinů z patogenů zapojených do rozpoznávání hostitelského organismu

Investor: Czech Science Foundation

GA15-17670S, research and development project

Name: Molekulární podstata pro specifické rozpoznávání histonu H3K4me2 pomocí Set3 PHD domény

Investor: Czech Science Foundation

LM2015043, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

JANČAŘÍKOVÁ, Gita, Josef HOUSER, Pavel DOBEŠ, Gabriel DEMO, Pavel HYRŠL and Michaela WIMMEROVÁ. Characterization of novel bangle lectin from Photorhabdus asymbiotica with dual sugar-binding specificity and its effect on host immunity. PLoS Pathogens. San Francisco: Public Library of Science, 2017, vol. 13, No 8, p. 1-28. ISSN 1553-7366. Available from: https://dx.doi.org/10.1371/journal.ppat.1006564.

@article{1387535,
   author = {Jančaříková, Gita and Houser, Josef and Dobeš, Pavel and Demo, Gabriel and Hyršl, Pavel and Wimmerová, Michaela},
   article_location = {San Francisco},
   article_number = {8},
   doi = {http://dx.doi.org/10.1371/journal.ppat.1006564},
   keywords = {Lectins; blood; insects; blood groups; carbohydrates; immune response; sedimenation; nematode infections},
   language = {eng},
   issn = {1553-7366},
   journal = {PLoS Pathogens},
   title = {Characterization of novel bangle lectin from Photorhabdus asymbiotica with dual sugar-binding specificity and its effect on host immunity},
   url = {http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006564},
   volume = {13},
   year = {2017}
}

TY  - JOUR
ID  - 1387535
AU  - Jančaříková, Gita - Houser, Josef - Dobeš, Pavel - Demo, Gabriel - Hyršl, Pavel - Wimmerová, Michaela
PY  - 2017
TI  - Characterization of novel bangle lectin from Photorhabdus asymbiotica with dual sugar-binding specificity and its effect on host immunity
JF  - PLoS Pathogens
VL  - 13
IS  - 8
SP  - 1-28
EP  - 1-28
PB  - Public Library of Science
SN  - 15537366
KW  - Lectins
KW  - blood
KW  - insects
KW  - blood groups
KW  - carbohydrates
KW  - immune response
KW  - sedimenation
KW  - nematode infections
UR  - http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006564
L2  - http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006564
N2  - Photorhabdus asymbiotica is one of the three recognized species of the Photorhabdus genus, which consists of gram-negative bioluminescent bacteria belonging to the family Morganellaceae. These bacteria live in a symbiotic relationship with nematodes from the genus Heterorhabditis, together forming a complex that is highly pathogenic for insects. Unlike other Photorhabdus species, which are strictly entomopathogenic, P. asymbiotica is unique in its ability to act as an emerging human pathogen. Analysis of the P. asymbiotica genome identified a novel fucose-binding lectin designated PHL with a strong sequence similarity to the recently described P. luminescens lectin PLL. Recombinant PHL exhibited high affinity for fucosylated carbohydrates and the unusual disaccharide 3,6-O-Me2-GlcBeta1± 4(2,3-O-Me2)RhaAlpha-O-(p-C6H4)-OCH2CH2NH2 from Mycobacterium leprae. Based on its crystal structure, PHL forms a seven-bladed beta-propeller assembling into a homo-dimer with an inter-subunit disulfide bridge. Investigating complexes with different ligands revealed the existence of two sets of binding sites per monomer - the first type prefers L-fucose and its derivatives, whereas the second type can bind D-galactose. Based on the sequence analysis, PHL could contain up to twelve binding sites per monomer. PHL was shown to interact with all types of red blood cells and insect haemocytes. Interestingly, PHL inhibited the production of reactive oxygen species induced by zymosan A in human blood and antimicrobial activity both in human blood, serum and insect haemolymph. Concurrently, PHL increased the constitutive level of oxidants in the blood and induced melanisation in haemolymph. Our results suggest that PHL might play a crucial role in the interaction of P. asymbiotica with both human and insect hosts.
ER  -

JANČAŘÍKOVÁ, Gita, Josef HOUSER, Pavel DOBEŠ, Gabriel DEMO, Pavel HYRŠL and Michaela WIMMEROVÁ. Characterization of novel bangle lectin from Photorhabdus asymbiotica with dual sugar-binding specificity and its effect on host immunity. \textit{PLoS Pathogens}. San Francisco: Public Library of Science, 2017, vol.~13, No~8, p.~1-28. ISSN~1553-7366. Available from: https://dx.doi.org/10.1371/journal.ppat.1006564.

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