KOVÁČIK, Jozef, Gabriela ROTKOVÁ, Marek BUJDOŠ, Petr BABULA, Viera PETERKOVÁ and Peter MATÚŠ. Ascorbic acid protects Coccomyxa subellipsoidea against metal toxicity through modulation of ROS/NO balance and metal uptake. JOURNAL OF HAZARDOUS MATERIALS. Amsterdam: Elsevier Science, vol. 339, October, p. 200-207. ISSN 0304-3894. doi:10.1016/j.jhazmat.2017.06.035. 2017.
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Basic information
Original name Ascorbic acid protects Coccomyxa subellipsoidea against metal toxicity through modulation of ROS/NO balance and metal uptake
Authors KOVÁČIK, Jozef (703 Slovakia), Gabriela ROTKOVÁ (203 Czech Republic, guarantor, belonging to the institution), Marek BUJDOŠ (703 Slovakia), Petr BABULA (203 Czech Republic, belonging to the institution), Viera PETERKOVÁ (703 Slovakia) and Peter MATÚŠ (703 Slovakia).
Edition JOURNAL OF HAZARDOUS MATERIALS, Amsterdam, Elsevier Science, 2017, 0304-3894.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30304 Public and environmental health
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 6.434
RIV identification code RIV/00216224:14310/17:00097366
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1016/j.jhazmat.2017.06.035
UT WoS 000407188200021
Keywords in English Antioxidants; Fluorescence microscopy; Heavy metals; Oxidative stress; RT-PCR
Tags NZ, rivok
Tags International impact, Reviewed
Changed by Changed by: Ing. Nicole Zrilić, učo 240776. Changed: 28/3/2018 16:28.
Abstract
Impact of exogenous ascorbic acid (AsA, 100 uM) on acute metal toxicity (Pb and Hg, 24 h of exposure to 100 uM) in unicellular green alga Coccomyxa subellipsoidea was studied. Hg (often extensively) depleted amount of pigments, potassium, soluble proteins, endogenous AsA, non-protein thiols and nitric oxide signal but elevated ROS signal and activities of catalase and superoxide dismutase. Responses to Pb application (if any) were less visible with ROS signal being slightly elevated and amount of non-protein thiols being slightly reduced. Exogenous AsA considerably improved mainly Hg-induced damage at the level of oxidative stress and physiological parameters and evoked an increase in nitric oxide signal. Maximum amount of total Hg was almost 4-fold higher than that of Pb (36.5 vs. 9.73 mg/g DW) and AsA depleted both total and intracellular accumulation. At the level of ascorbate-related proteins, exogenous AsA suppressed metal-induced expression and activity of MDHAR and APX but not of DHAR, which may indicate a role of DHAR in algal stress tolerance. Our data suggest that given Coccomyxa species is suitable candidate for remediation of Hg or Pb and that ascorbic acid effectively ameliorates metal-induced toxicity without side effects.
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