Ascorbic acid protects Coccomyxa subellipsoidea against metal
toxicity through modulation of ROS/NO balance and metal uptake

J 2017

Ascorbic acid protects Coccomyxa subellipsoidea against metal toxicity through modulation of ROS/NO balance and metal uptake

KOVÁČIK, Jozef, Gabriela ROTKOVÁ, Marek BUJDOŠ, Petr BABULA, Viera PETERKOVÁ et. al.

Basic information

Original name

Ascorbic acid protects Coccomyxa subellipsoidea against metal toxicity through modulation of ROS/NO balance and metal uptake

Authors

KOVÁČIK, Jozef (703 Slovakia), Gabriela ROTKOVÁ (203 Czech Republic, guarantor, belonging to the institution), Marek BUJDOŠ (703 Slovakia), Petr BABULA (203 Czech Republic, belonging to the institution), Viera PETERKOVÁ (703 Slovakia) and Peter MATÚŠ (703 Slovakia)

Edition

JOURNAL OF HAZARDOUS MATERIALS, Amsterdam, Elsevier Science, 2017, 0304-3894

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30304 Public and environmental health

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 6.434

RIV identification code

RIV/00216224:14310/17:00097366

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.jhazmat.2017.06.035

UT WoS

000407188200021

Keywords in English

Antioxidants; Fluorescence microscopy; Heavy metals; Oxidative stress; RT-PCR

Abstract

V originále

Impact of exogenous ascorbic acid (AsA, 100 uM) on acute metal toxicity (Pb and Hg, 24 h of exposure to 100 uM) in unicellular green alga Coccomyxa subellipsoidea was studied. Hg (often extensively) depleted amount of pigments, potassium, soluble proteins, endogenous AsA, non-protein thiols and nitric oxide signal but elevated ROS signal and activities of catalase and superoxide dismutase. Responses to Pb application (if any) were less visible with ROS signal being slightly elevated and amount of non-protein thiols being slightly reduced. Exogenous AsA considerably improved mainly Hg-induced damage at the level of oxidative stress and physiological parameters and evoked an increase in nitric oxide signal. Maximum amount of total Hg was almost 4-fold higher than that of Pb (36.5 vs. 9.73 mg/g DW) and AsA depleted both total and intracellular accumulation. At the level of ascorbate-related proteins, exogenous AsA suppressed metal-induced expression and activity of MDHAR and APX but not of DHAR, which may indicate a role of DHAR in algal stress tolerance. Our data suggest that given Coccomyxa species is suitable candidate for remediation of Hg or Pb and that ascorbic acid effectively ameliorates metal-induced toxicity without side effects.

Citovat

KOVÁČIK, Jozef, Gabriela ROTKOVÁ, Marek BUJDOŠ, Petr BABULA, Viera PETERKOVÁ and Peter MATÚŠ. Ascorbic acid protects Coccomyxa subellipsoidea against metal toxicity through modulation of ROS/NO balance and metal uptake. JOURNAL OF HAZARDOUS MATERIALS. Amsterdam: Elsevier Science, 2017, vol. 339, October, p. 200-207. ISSN 0304-3894. Available from: https://dx.doi.org/10.1016/j.jhazmat.2017.06.035.

@article{1387611,
   author = {Kováčik, Jozef and Rotková, Gabriela and Bujdoš, Marek and Babula, Petr and Peterková, Viera and Matúš, Peter},
   article_location = {Amsterdam},
   article_number = {October},
   doi = {http://dx.doi.org/10.1016/j.jhazmat.2017.06.035},
   keywords = {Antioxidants; Fluorescence microscopy; Heavy metals; Oxidative stress; RT-PCR},
   language = {eng},
   issn = {0304-3894},
   journal = {JOURNAL OF HAZARDOUS MATERIALS},
   title = {Ascorbic acid protects Coccomyxa subellipsoidea against metal toxicity through modulation of ROS/NO balance and metal uptake},
   volume = {339},
   year = {2017}
}

TY  - JOUR
ID  - 1387611
AU  - Kováčik, Jozef - Rotková, Gabriela - Bujdoš, Marek - Babula, Petr - Peterková, Viera - Matúš, Peter
PY  - 2017
TI  - Ascorbic acid protects Coccomyxa subellipsoidea against metal toxicity through modulation of ROS/NO balance and metal uptake
JF  - JOURNAL OF HAZARDOUS MATERIALS
VL  - 339
IS  - October
SP  - 200-207
EP  - 200-207
PB  - Elsevier Science
SN  - 03043894
KW  - Antioxidants
KW  - Fluorescence microscopy
KW  - Heavy metals
KW  - Oxidative stress
KW  - RT-PCR
N2  - Impact of exogenous ascorbic acid (AsA, 100 uM) on acute metal toxicity (Pb and Hg, 24 h of exposure to 100 uM) in unicellular green alga Coccomyxa subellipsoidea was studied. Hg (often extensively) depleted amount of pigments, potassium, soluble proteins, endogenous AsA, non-protein thiols and nitric oxide signal but elevated ROS signal and activities of catalase and superoxide dismutase. Responses to Pb application (if any) were less visible with ROS signal being slightly elevated and amount of non-protein thiols being slightly reduced. Exogenous AsA considerably improved mainly Hg-induced damage at the level of oxidative stress and physiological parameters and evoked an increase in nitric oxide signal. Maximum amount of total Hg was almost 4-fold higher than that of Pb (36.5 vs. 9.73 mg/g DW) and AsA depleted both total and intracellular accumulation. At the level of ascorbate-related proteins, exogenous AsA suppressed metal-induced expression and activity of MDHAR and APX but not of DHAR, which may indicate a role of DHAR in algal stress tolerance. Our data suggest that given Coccomyxa species is suitable candidate for remediation of Hg or Pb and that ascorbic acid effectively ameliorates metal-induced toxicity without side effects.
ER  -

KOVÁČIK, Jozef, Gabriela ROTKOVÁ, Marek BUJDOŠ, Petr BABULA, Viera PETERKOVÁ and Peter MATÚŠ. Ascorbic acid protects Coccomyxa subellipsoidea against metal toxicity through modulation of ROS/NO balance and metal uptake. \textit{JOURNAL OF HAZARDOUS MATERIALS}. Amsterdam: Elsevier Science, 2017, vol.~339, October, p.~200-207. ISSN~0304-3894. Available from: https://dx.doi.org/10.1016/j.jhazmat.2017.06.035.

Detailed Information on Publication Record