Luminescent Ruthenium(II) Polypyridyl Complexes as Nonviral
Carriers for DNA Delivery

J 2017

Luminescent Ruthenium(II) Polypyridyl Complexes as Nonviral Carriers for DNA Delivery

BHAT, Satish S., Vidyanand K. REVANKAR, Ayesha KHAN, Rahul V. PINJARI, Marek NEČAS et. al.

Basic information

Original name

Luminescent Ruthenium(II) Polypyridyl Complexes as Nonviral Carriers for DNA Delivery

Authors

BHAT, Satish S. (356 India), Vidyanand K. REVANKAR (356 India), Ayesha KHAN (356 India), Rahul V. PINJARI (356 India) and Marek NEČAS (203 Czech Republic, guarantor, belonging to the institution)

Edition

Chemistry - An Asian Journal, WEINHEIM, WILEY-V C H VERLAG GMBH, 2017, 1861-4728

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10402 Inorganic and nuclear chemistry

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.692

RIV identification code

RIV/00216224:14310/17:00097387

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1002/asia.201601515

UT WoS

000393434200014

Keywords in English

DNA; electrochemistry; luminescence; photochemistry; ruthenium

Abstract

V originále

Two new luminescent ruthenium(II) polypyridyl complexes, [Ru(bpy)(2)(tpt-phen)]Cl-2 (1; bpy=2,2'-bipyridine, tpt-phen=triptycenyl-1,10-phenanthroline) and [Ru(phen)(2)(tpt-phen)]Cl-2 (2; phen=1,10-phenanthroline), have been developed as potential nonviral vectors for DNA delivery. Photophysical and electrochemical properties of the complexes have been investigated and corroborated with electronic structure calculations. DNA condensation by these complexes has been investigated by UV/Vis and emission spectroscopy, circular dichroism spectroscopy, atomic force microscopy, dynamic light scattering, confocal microscopy, and electrophoretic mobility studies. These complexes interact with DNA and efficiently condense DNA into globular nanoparticles that are taken up efficiently by HeLa cells. DNA cleavage inability and biocompatibility of complexes have been explored. Both complexes have good gene transfection abilities.

Links

LM2015043, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

BHAT, Satish S., Vidyanand K. REVANKAR, Ayesha KHAN, Rahul V. PINJARI and Marek NEČAS. Luminescent Ruthenium(II) Polypyridyl Complexes as Nonviral Carriers for DNA Delivery. Chemistry - An Asian Journal. WEINHEIM: WILEY-V C H VERLAG GMBH, 2017, vol. 12, No 2, p. 254-264. ISSN 1861-4728. Available from: https://dx.doi.org/10.1002/asia.201601515.

@article{1387865,
   author = {Bhat, Satish S. and Revankar, Vidyanand K. and Khan, Ayesha and Pinjari, Rahul V. and Nečas, Marek},
   article_location = {WEINHEIM},
   article_number = {2},
   doi = {http://dx.doi.org/10.1002/asia.201601515},
   keywords = {DNA; electrochemistry; luminescence; photochemistry; ruthenium},
   language = {eng},
   issn = {1861-4728},
   journal = {Chemistry - An Asian Journal},
   title = {Luminescent Ruthenium(II) Polypyridyl Complexes as Nonviral Carriers for DNA Delivery},
   volume = {12},
   year = {2017}
}

TY  - JOUR
ID  - 1387865
AU  - Bhat, Satish S. - Revankar, Vidyanand K. - Khan, Ayesha - Pinjari, Rahul V. - Nečas, Marek
PY  - 2017
TI  - Luminescent Ruthenium(II) Polypyridyl Complexes as Nonviral Carriers for DNA Delivery
JF  - Chemistry - An Asian Journal
VL  - 12
IS  - 2
SP  - 254-264
EP  - 254-264
PB  - WILEY-V C H VERLAG GMBH
SN  - 18614728
KW  - DNA
KW  - electrochemistry
KW  - luminescence
KW  - photochemistry
KW  - ruthenium
N2  - Two new luminescent ruthenium(II) polypyridyl complexes, [Ru(bpy)(2)(tpt-phen)]Cl-2 (1; bpy=2,2'-bipyridine, tpt-phen=triptycenyl-1,10-phenanthroline) and [Ru(phen)(2)(tpt-phen)]Cl-2 (2; phen=1,10-phenanthroline), have been developed as potential nonviral vectors for DNA delivery. Photophysical and electrochemical properties of the complexes have been investigated and corroborated with electronic structure calculations. DNA condensation by these complexes has been investigated by UV/Vis and emission spectroscopy, circular dichroism spectroscopy, atomic force microscopy, dynamic light scattering, confocal microscopy, and electrophoretic mobility studies. These complexes interact with DNA and efficiently condense DNA into globular nanoparticles that are taken up efficiently by HeLa cells. DNA cleavage inability and biocompatibility of complexes have been explored. Both complexes have good gene transfection abilities.
ER  -

BHAT, Satish S., Vidyanand K. REVANKAR, Ayesha KHAN, Rahul V. PINJARI and Marek NEČAS. Luminescent Ruthenium(II) Polypyridyl Complexes as Nonviral Carriers for DNA Delivery. \textit{Chemistry - An Asian Journal}. WEINHEIM: WILEY-V C H VERLAG GMBH, 2017, vol.~12, No~2, p.~254-264. ISSN~1861-4728. Available from: https://dx.doi.org/10.1002/asia.201601515.

Detailed Information on Publication Record