Detailed Information on Publication Record
2017
Different Densities of Na-Ca Exchange Current in T-Tubular and Surface Membranes and Their Impact on Cellular Activity in a Model of Rat Ventricular Cardiomyocyte
PÁSEK, Michal, Jiří ŠIMURDA and G. CHRISTÉBasic information
Original name
Different Densities of Na-Ca Exchange Current in T-Tubular and Surface Membranes and Their Impact on Cellular Activity in a Model of Rat Ventricular Cardiomyocyte
Authors
PÁSEK, Michal (203 Czech Republic, guarantor, belonging to the institution), Jiří ŠIMURDA (203 Czech Republic, belonging to the institution) and G. CHRISTÉ (250 France)
Edition
Biomed Research International, New York, Hindawi Publishing Corporation, 2017, 2314-6133
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30400 3.4 Medical biotechnology
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.583
RIV identification code
RIV/00216224:14110/17:00095655
Organization unit
Faculty of Medicine
UT WoS
000396222700001
Keywords in English
SODIUM-CALCIUM EXCHANGE; NA+-CA2+ EXCHANGE; CARDIAC MYOCYTES; FAILING HEARTS; NA/CA EXCHANGE; PROTEIN; CELLS; LOCALIZATION; MECHANISM; RELEASE
Tags
Tags
International impact, Reviewed
Změněno: 20/3/2018 18:31, Soňa Böhmová
Abstract
V originále
The ratio of densities of Na-Ca exchanger current (I-NaCa) in the t-tubular and surface membranes (I-NaCa-ratio) computed from the values of I-NaCa and membrane capacitances (C-m) measured in adult rat ventricular cardiomyocytes before and after detubulation ranges between 1.7 and 25 (potentially even 40). Variations of action potential waveform and of calcium turnover within this span of the I-NaCa-ratio were simulated employing previously developed model of rat ventricular cell incorporating separate description of ion transport systems in the t-tubular and surface membranes. The increase of I-NaCa-ratio from 1.7 to 25 caused a prolongation of APD (duration of action potential at 90% repolarisation) by 12, 9, and 6% and an increase of peak intracellular Ca2+ transient by 45, 19, and 6% at 0.1, 1, and 5Hz, respectively. The prolonged APD resulted from the increase of I-NaCa due to the exposure of a larger fraction of Na-Ca exchangers to higher Ca2+ transients under the t-tubular membrane. The accompanying rise of Ca2+ transient was a consequence of a higher Ca2+ load in sarcoplasmic reticulum induced by the increased Ca2+ cycling between the surface and t-tubular membranes. However, the reason for large differences in the I-NaCa-ratio assessed from measurements in adult rat cardiomyocytes remains to be explained.
Links
| NV16-30571A, research and development project |
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