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@proceedings{1388127,
author = {Vorel, Jiří and Roudnický, Pavel and Ilgová, Jana and Dvořáková, Hana and Jedličková, Lucie and Mikeš, Libor and Brož, Petr and Jirsová, Dagmar and Leontovyč, Roman and Vetešník, Lukáš and Jurajda, Pavel and Jankůjová, Marie and Oppelt, Jan and Koubková, Božena and Gelnar, Milan and Kašný, Martin},
booktitle = {8th International Symposium on Monogenea},
keywords = {Sequencing; transcriptomics; helminths; Eudiplozoon nipponicum; Monogenea},
language = {eng},
isbn = {978-80-210-8666-1},
title = {Eudiplozoon nipponicum (Polyopisthocotylea: Diplozoidae): transcriptome and secretome analyses of hematophagous fish parasite},
url = {http://ecip.cz/news/4},
year = {2017}
}
TY - CONF
ID - 1388127
AU - Vorel, Jiří - Roudnický, Pavel - Ilgová, Jana - Dvořáková, Hana - Jedličková, Lucie - Mikeš, Libor - Brož, Petr - Jirsová, Dagmar - Leontovyč, Roman - Vetešník, Lukáš - Jurajda, Pavel - Jankůjová, Marie - Oppelt, Jan - Koubková, Božena - Gelnar, Milan - Kašný, Martin
PY - 2017
TI - Eudiplozoon nipponicum (Polyopisthocotylea: Diplozoidae): transcriptome and secretome analyses of hematophagous fish parasite
SN - 9788021086661
KW - Sequencing
KW - transcriptomics
KW - helminths
KW - Eudiplozoon nipponicum
KW - Monogenea
UR - http://ecip.cz/news/4
L2 - http://ecip.cz/news/4
N2 - Two sequencing methods (454/Roche and Illumina MiSeq platform) and mass spectrometry analysis (HPLC MS/MS) were used. Total RNA from a few E. nipponicum adults, in the form of cDNA, was sequenced and Illumina and 454/Roche raw reads were processed and assembled separately using specific bioinformatics tools. Based on statistical evaluation, selected assembly variants were polled and filtered, in order to get high quality transcriptomics sequences for further annotation, into final contaminant-free 37,062 transcripts with corresponding translated proteins. Totally 19,539 (52.7 %) transcripts were homologous to some record in used public sequence databases (BLASTn and BLASTp algorithms were used, E-value cut-off: 1e-5) and 18,556 (50.1 %) of them were linked with sequences related to organisms only in phylum Platyhelminthes deposited in UniProtKB/TrEMBL database. All transcripts were deeply annotated by established approach. Excretory-secretory products (ESP) – protein compounds present in parasite secretome, were obtained from ~100 living adults. HPLC MS/MS analysis of peptide mixture were done using RSLCnano System connected to Orbitrap Elite Hybrid spectrometer after tryptic digestion. The analysis of the mass spectrometric raw data was carried out using the Proteome Discoverer software tool with Mascot search engine utilisation and finally, 1,033 transcripts were identified in secretome.
ER -
VOREL, Jiří, Pavel ROUDNICKÝ, Jana ILGOVÁ, Hana DVOŘÁKOVÁ, Lucie JEDLIČKOVÁ, Libor MIKEŠ, Petr BROŽ, Dagmar JIRSOVÁ, Roman LEONTOVYČ, Lukáš VETEŠNÍK, Pavel JURAJDA, Marie JANKŮJOVÁ, Jan OPPELT, Božena KOUBKOVÁ, Milan GELNAR and Martin KAŠNÝ. Eudiplozoon nipponicum (Polyopisthocotylea: Diplozoidae): transcriptome and secretome analyses of hematophagous fish parasite. In \textit{8th International Symposium on Monogenea}. 2017. ISBN~978-80-210-8666-1.
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