Detailed Information on Publication Record
2017
Eudiplozoon nipponicum (Polyopisthocotylea: Diplozoidae): transcriptome and secretome analyses of hematophagous fish parasite
VOREL, Jiří, Pavel ROUDNICKÝ, Jana ILGOVÁ, Hana DVOŘÁKOVÁ, Lucie JEDLIČKOVÁ et. al.Basic information
Original name
Eudiplozoon nipponicum (Polyopisthocotylea: Diplozoidae): transcriptome and secretome analyses of hematophagous fish parasite
Authors
VOREL, Jiří (203 Czech Republic, guarantor, belonging to the institution), Pavel ROUDNICKÝ (203 Czech Republic, belonging to the institution), Jana ILGOVÁ (703 Slovakia, belonging to the institution), Hana DVOŘÁKOVÁ (203 Czech Republic), Lucie JEDLIČKOVÁ (203 Czech Republic), Libor MIKEŠ (203 Czech Republic), Petr BROŽ (203 Czech Republic), Dagmar JIRSOVÁ (203 Czech Republic), Roman LEONTOVYČ (203 Czech Republic), Lukáš VETEŠNÍK (203 Czech Republic), Pavel JURAJDA (203 Czech Republic), Marie JANKŮJOVÁ (203 Czech Republic), Jan OPPELT (203 Czech Republic), Božena KOUBKOVÁ (203 Czech Republic), Milan GELNAR (203 Czech Republic) and Martin KAŠNÝ (203 Czech Republic)
Edition
8th International Symposium on Monogenea, 2017
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
RIV identification code
RIV/00216224:14310/17:00094901
Organization unit
Faculty of Science
ISBN
978-80-210-8666-1
Keywords in English
Sequencing; transcriptomics; helminths; Eudiplozoon nipponicum; Monogenea
Tags
International impact
Změněno: 28/8/2017 09:55, Mgr. Jiří Vorel, Ph.D.
Abstract
V originále
Two sequencing methods (454/Roche and Illumina MiSeq platform) and mass spectrometry analysis (HPLC MS/MS) were used. Total RNA from a few E. nipponicum adults, in the form of cDNA, was sequenced and Illumina and 454/Roche raw reads were processed and assembled separately using specific bioinformatics tools. Based on statistical evaluation, selected assembly variants were polled and filtered, in order to get high quality transcriptomics sequences for further annotation, into final contaminant-free 37,062 transcripts with corresponding translated proteins. Totally 19,539 (52.7 %) transcripts were homologous to some record in used public sequence databases (BLASTn and BLASTp algorithms were used, E-value cut-off: 1e-5) and 18,556 (50.1 %) of them were linked with sequences related to organisms only in phylum Platyhelminthes deposited in UniProtKB/TrEMBL database. All transcripts were deeply annotated by established approach. Excretory-secretory products (ESP) – protein compounds present in parasite secretome, were obtained from ~100 living adults. HPLC MS/MS analysis of peptide mixture were done using RSLCnano System connected to Orbitrap Elite Hybrid spectrometer after tryptic digestion. The analysis of the mass spectrometric raw data was carried out using the Proteome Discoverer software tool with Mascot search engine utilisation and finally, 1,033 transcripts were identified in secretome.
Links
GBP505/12/G112, research and development project |
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