Eudiplozoon nipponicum (Polyopisthocotylea: Diplozoidae):
transcriptome and secretome analyses of hematophagous fish
parasite

a 2017

Eudiplozoon nipponicum (Polyopisthocotylea: Diplozoidae): transcriptome and secretome analyses of hematophagous fish parasite

VOREL, Jiří, Pavel ROUDNICKÝ, Jana ILGOVÁ, Hana DVOŘÁKOVÁ, Lucie JEDLIČKOVÁ et. al.

Basic information

Original name

Eudiplozoon nipponicum (Polyopisthocotylea: Diplozoidae): transcriptome and secretome analyses of hematophagous fish parasite

Authors

VOREL, Jiří (203 Czech Republic, guarantor, belonging to the institution), Pavel ROUDNICKÝ (203 Czech Republic, belonging to the institution), Jana ILGOVÁ (703 Slovakia, belonging to the institution), Hana DVOŘÁKOVÁ (203 Czech Republic), Lucie JEDLIČKOVÁ (203 Czech Republic), Libor MIKEŠ (203 Czech Republic), Petr BROŽ (203 Czech Republic), Dagmar JIRSOVÁ (203 Czech Republic), Roman LEONTOVYČ (203 Czech Republic), Lukáš VETEŠNÍK (203 Czech Republic), Pavel JURAJDA (203 Czech Republic), Marie JANKŮJOVÁ (203 Czech Republic), Jan OPPELT (203 Czech Republic), Božena KOUBKOVÁ (203 Czech Republic), Milan GELNAR (203 Czech Republic) and Martin KAŠNÝ (203 Czech Republic)

Edition

8th International Symposium on Monogenea, 2017

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

ECIP - European Centre of IchthyoParasitology

RIV identification code

RIV/00216224:14310/17:00094901

Organization unit

Faculty of Science

ISBN

978-80-210-8666-1

Keywords in English

Sequencing; transcriptomics; helminths; Eudiplozoon nipponicum; Monogenea

Abstract

V originále

Two sequencing methods (454/Roche and Illumina MiSeq platform) and mass spectrometry analysis (HPLC MS/MS) were used. Total RNA from a few E. nipponicum adults, in the form of cDNA, was sequenced and Illumina and 454/Roche raw reads were processed and assembled separately using specific bioinformatics tools. Based on statistical evaluation, selected assembly variants were polled and filtered, in order to get high quality transcriptomics sequences for further annotation, into final contaminant-free 37,062 transcripts with corresponding translated proteins. Totally 19,539 (52.7 %) transcripts were homologous to some record in used public sequence databases (BLASTn and BLASTp algorithms were used, E-value cut-off: 1e-5) and 18,556 (50.1 %) of them were linked with sequences related to organisms only in phylum Platyhelminthes deposited in UniProtKB/TrEMBL database. All transcripts were deeply annotated by established approach. Excretory-secretory products (ESP) – protein compounds present in parasite secretome, were obtained from ~100 living adults. HPLC MS/MS analysis of peptide mixture were done using RSLCnano System connected to Orbitrap Elite Hybrid spectrometer after tryptic digestion. The analysis of the mass spectrometric raw data was carried out using the Proteome Discoverer software tool with Mascot search engine utilisation and finally, 1,033 transcripts were identified in secretome.

Links

GBP505/12/G112, research and development project

Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

Citovat

VOREL, Jiří, Pavel ROUDNICKÝ, Jana ILGOVÁ, Hana DVOŘÁKOVÁ, Lucie JEDLIČKOVÁ, Libor MIKEŠ, Petr BROŽ, Dagmar JIRSOVÁ, Roman LEONTOVYČ, Lukáš VETEŠNÍK, Pavel JURAJDA, Marie JANKŮJOVÁ, Jan OPPELT, Božena KOUBKOVÁ, Milan GELNAR and Martin KAŠNÝ. Eudiplozoon nipponicum (Polyopisthocotylea: Diplozoidae): transcriptome and secretome analyses of hematophagous fish parasite. In 8th International Symposium on Monogenea. 2017. ISBN 978-80-210-8666-1.

@proceedings{1388127,
   author = {Vorel, Jiří and Roudnický, Pavel and Ilgová, Jana and Dvořáková, Hana and Jedličková, Lucie and Mikeš, Libor and Brož, Petr and Jirsová, Dagmar and Leontovyč, Roman and Vetešník, Lukáš and Jurajda, Pavel and Jankůjová, Marie and Oppelt, Jan and Koubková, Božena and Gelnar, Milan and Kašný, Martin},
   booktitle = {8th International Symposium on Monogenea},
   keywords = {Sequencing; transcriptomics; helminths; Eudiplozoon nipponicum; Monogenea},
   language = {eng},
   isbn = {978-80-210-8666-1},
   title = {Eudiplozoon nipponicum (Polyopisthocotylea: Diplozoidae): transcriptome and secretome analyses of hematophagous fish parasite},
   url = {http://ecip.cz/news/4},
   year = {2017}
}

TY  - CONF
ID  - 1388127
AU  - Vorel, Jiří - Roudnický, Pavel - Ilgová, Jana - Dvořáková, Hana - Jedličková, Lucie - Mikeš, Libor - Brož, Petr - Jirsová, Dagmar - Leontovyč, Roman - Vetešník, Lukáš - Jurajda, Pavel - Jankůjová, Marie - Oppelt, Jan - Koubková, Božena - Gelnar, Milan - Kašný, Martin
PY  - 2017
TI  - Eudiplozoon nipponicum (Polyopisthocotylea: Diplozoidae): transcriptome and secretome analyses of hematophagous fish parasite
SN  - 9788021086661
KW  - Sequencing
KW  - transcriptomics
KW  - helminths
KW  - Eudiplozoon nipponicum
KW  - Monogenea
UR  - http://ecip.cz/news/4
L2  - http://ecip.cz/news/4
N2  - Two sequencing methods (454/Roche and Illumina MiSeq platform) and mass spectrometry analysis (HPLC MS/MS) were used. Total RNA from a few E. nipponicum adults, in the form of cDNA, was sequenced and Illumina and 454/Roche raw reads were processed and assembled separately using specific bioinformatics tools. Based on statistical evaluation, selected assembly variants were polled and filtered, in order to get high quality transcriptomics sequences for further annotation, into final contaminant-free 37,062 transcripts with corresponding translated proteins. Totally 19,539 (52.7 %) transcripts were homologous to some record in used public sequence databases (BLASTn and BLASTp algorithms were used, E-value cut-off: 1e-5) and 18,556 (50.1 %) of them were linked with sequences related to organisms only in phylum Platyhelminthes deposited in UniProtKB/TrEMBL database. All transcripts were deeply annotated by established approach. Excretory-secretory products (ESP) – protein compounds present in parasite secretome, were obtained from ~100 living adults. HPLC MS/MS analysis of peptide mixture were done using RSLCnano System connected to Orbitrap Elite Hybrid spectrometer after tryptic digestion. The analysis of the mass spectrometric raw data was carried out using the Proteome Discoverer software tool with Mascot search engine utilisation and finally, 1,033 transcripts were identified in secretome.
ER  -

VOREL, Jiří, Pavel ROUDNICKÝ, Jana ILGOVÁ, Hana DVOŘÁKOVÁ, Lucie JEDLIČKOVÁ, Libor MIKEŠ, Petr BROŽ, Dagmar JIRSOVÁ, Roman LEONTOVYČ, Lukáš VETEŠNÍK, Pavel JURAJDA, Marie JANKŮJOVÁ, Jan OPPELT, Božena KOUBKOVÁ, Milan GELNAR and Martin KAŠNÝ. Eudiplozoon nipponicum (Polyopisthocotylea: Diplozoidae): transcriptome and secretome analyses of hematophagous fish parasite. In \textit{8th International Symposium on Monogenea}. 2017. ISBN~978-80-210-8666-1.

Detailed Information on Publication Record