Fluorescence microscopy as a tool for visualization of
metal-induced oxidative stress in plants

J 2017

Fluorescence microscopy as a tool for visualization of metal-induced oxidative stress in plants

KOVACIK, Jozef and Petr BABULA

Basic information

Original name

Fluorescence microscopy as a tool for visualization of metal-induced oxidative stress in plants

Authors

KOVACIK, Jozef (703 Slovakia) and Petr BABULA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Acta Physiologiae Plantarum, Cracow, Polish Academy of Sciences, 2017, 0137-5881

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10700 1.7 Other natural sciences

Country of publisher

Poland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.438

RIV identification code

RIV/00216224:14110/17:00097480

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s11738-017-2455-0

UT WoS

000405471300002

Keywords in English

Bioaccumulation; Heavy metals; Maize; Reactive oxygen species; Root anatomy

Abstract

V originále

Various fluorescence reagents are often used for the detection of oxidative stress, but more extensive comparison in the same study is rather rare. We tested five ROS-related and two non-ROS-related reagents in the roots of Zea mays germinated and growing in 1 or 100 mu M cadmium-enriched solution. Namely, "general'' ROS (CellROX Deep Red reagent and 20,70-dichlorodihydrofluorescein diacetate), hydrogen peroxide (Amplex UltraRed), superoxide radical (dihydroethidium), hydroxyl radical/peroxynitrite (aminophenyl fluorescein), nitric oxide (1,2-diaminoanthraquinone), and glutathione/-SH (monochlorobimane) indicators were tested. Both Cd doses stimulated signal of various ROS often extensively compared to control and mainly H2O2 differed between 1 and 100 mu M Cd. Signal of ROS was clearly visible in exodermis and vascular tissue through which cadmium is transported via transpiration stream. CellROX Deep Red reagent gives bright and clear signal on the root cross sections. The standard spectrophotometry (detection of H2O2 and superoxide) did not reveal differences between control and 1 mu M Cd, indicating that fluorescence microscopy is more sensitive to detect low change in oxidative balance. Nitric oxide and glutathione/SH signal was less different between control and Cd treatments, indicating their contribution to metal tolerance. Cd accumulation and translocation and the use of reagents under the excess of other metals are also discussed.

Citovat

KOVACIK, Jozef and Petr BABULA. Fluorescence microscopy as a tool for visualization of metal-induced oxidative stress in plants. Acta Physiologiae Plantarum. Cracow: Polish Academy of Sciences, 2017, vol. 39, No 8, p. 1-7. ISSN 0137-5881. Available from: https://dx.doi.org/10.1007/s11738-017-2455-0.

@article{1388718,
   author = {Kovacik, Jozef and Babula, Petr},
   article_location = {Cracow},
   article_number = {8},
   doi = {http://dx.doi.org/10.1007/s11738-017-2455-0},
   keywords = {Bioaccumulation; Heavy metals; Maize; Reactive oxygen species; Root anatomy},
   language = {eng},
   issn = {0137-5881},
   journal = {Acta Physiologiae Plantarum},
   title = {Fluorescence microscopy as a tool for visualization of metal-induced oxidative stress in plants},
   volume = {39},
   year = {2017}
}

TY  - JOUR
ID  - 1388718
AU  - Kovacik, Jozef - Babula, Petr
PY  - 2017
TI  - Fluorescence microscopy as a tool for visualization of metal-induced oxidative stress in plants
JF  - Acta Physiologiae Plantarum
VL  - 39
IS  - 8
SP  - 1-7
EP  - 1-7
PB  - Polish Academy of Sciences
SN  - 01375881
KW  - Bioaccumulation
KW  - Heavy metals
KW  - Maize
KW  - Reactive oxygen species
KW  - Root anatomy
N2  - Various fluorescence reagents are often used for the detection of oxidative stress, but more extensive comparison in the same study is rather rare. We tested five ROS-related and two non-ROS-related reagents in the roots of Zea mays germinated and growing in 1 or 100 mu M cadmium-enriched solution. Namely, "general'' ROS (CellROX Deep Red reagent and 20,70-dichlorodihydrofluorescein diacetate), hydrogen peroxide (Amplex UltraRed), superoxide radical (dihydroethidium), hydroxyl radical/peroxynitrite (aminophenyl fluorescein), nitric oxide (1,2-diaminoanthraquinone), and glutathione/-SH (monochlorobimane) indicators were tested. Both Cd doses stimulated signal of various ROS often extensively compared to control and mainly H2O2 differed between 1 and 100 mu M Cd. Signal of ROS was clearly visible in exodermis and vascular tissue through which cadmium is transported via transpiration stream. CellROX Deep Red reagent gives bright and clear signal on the root cross sections. The standard spectrophotometry (detection of H2O2 and superoxide) did not reveal differences between control and 1 mu M Cd, indicating that fluorescence microscopy is more sensitive to detect low change in oxidative balance. Nitric oxide and glutathione/SH signal was less different between control and Cd treatments, indicating their contribution to metal tolerance. Cd accumulation and translocation and the use of reagents under the excess of other metals are also discussed.
ER  -

KOVACIK, Jozef and Petr BABULA. Fluorescence microscopy as a tool for visualization of metal-induced oxidative stress in plants. \textit{Acta Physiologiae Plantarum}. Cracow: Polish Academy of Sciences, 2017, vol.~39, No~8, p.~1-7. ISSN~0137-5881. Available from: https://dx.doi.org/10.1007/s11738-017-2455-0.

Detailed Information on Publication Record