KOVACIK, Jozef and Petr BABULA. Fluorescence microscopy as a tool for visualization of metal-induced oxidative stress in plants. Acta Physiologiae Plantarum. Cracow: Polish Academy of Sciences, 2017, vol. 39, No 8, p. 1-7. ISSN 0137-5881. Available from: https://dx.doi.org/10.1007/s11738-017-2455-0.
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Basic information
Original name Fluorescence microscopy as a tool for visualization of metal-induced oxidative stress in plants
Authors KOVACIK, Jozef (703 Slovakia) and Petr BABULA (203 Czech Republic, guarantor, belonging to the institution).
Edition Acta Physiologiae Plantarum, Cracow, Polish Academy of Sciences, 2017, 0137-5881.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10700 1.7 Other natural sciences
Country of publisher Poland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 1.438
RIV identification code RIV/00216224:14110/17:00097480
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1007/s11738-017-2455-0
UT WoS 000405471300002
Keywords in English Bioaccumulation; Heavy metals; Maize; Reactive oxygen species; Root anatomy
Tags EL OK
Tags International impact, Reviewed
Changed by Changed by: Soňa Böhmová, učo 232884. Changed: 7/3/2018 16:20.
Abstract
Various fluorescence reagents are often used for the detection of oxidative stress, but more extensive comparison in the same study is rather rare. We tested five ROS-related and two non-ROS-related reagents in the roots of Zea mays germinated and growing in 1 or 100 mu M cadmium-enriched solution. Namely, "general'' ROS (CellROX Deep Red reagent and 20,70-dichlorodihydrofluorescein diacetate), hydrogen peroxide (Amplex UltraRed), superoxide radical (dihydroethidium), hydroxyl radical/peroxynitrite (aminophenyl fluorescein), nitric oxide (1,2-diaminoanthraquinone), and glutathione/-SH (monochlorobimane) indicators were tested. Both Cd doses stimulated signal of various ROS often extensively compared to control and mainly H2O2 differed between 1 and 100 mu M Cd. Signal of ROS was clearly visible in exodermis and vascular tissue through which cadmium is transported via transpiration stream. CellROX Deep Red reagent gives bright and clear signal on the root cross sections. The standard spectrophotometry (detection of H2O2 and superoxide) did not reveal differences between control and 1 mu M Cd, indicating that fluorescence microscopy is more sensitive to detect low change in oxidative balance. Nitric oxide and glutathione/SH signal was less different between control and Cd treatments, indicating their contribution to metal tolerance. Cd accumulation and translocation and the use of reagents under the excess of other metals are also discussed.
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