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@article{1388721, author = {Kováčik, Jozef and Klejdus, Bořivoj and Babula, Petr and Hedbavny, Josef}, article_location = {Amsterdam}, article_number = {JUN 2017}, doi = {http://dx.doi.org/10.1016/j.algal.2017.04.026}, keywords = {Antioxidants; Fluorescence microscopy; Heavy metals; Krebs cycle acids; Mass spectrometry}, language = {eng}, issn = {2211-9264}, journal = {Algal Research - Biomass Biofuels and Bioproducts}, title = {Ascorbic acid affects short-term response of Scenedesmus quadricauda to cadmium excess}, volume = {24}, year = {2017} }
TY - JOUR ID - 1388721 AU - Kováčik, Jozef - Klejdus, Bořivoj - Babula, Petr - Hedbavny, Josef PY - 2017 TI - Ascorbic acid affects short-term response of Scenedesmus quadricauda to cadmium excess JF - Algal Research - Biomass Biofuels and Bioproducts VL - 24 IS - JUN 2017 SP - 354-359 EP - 354-359 PB - Elsevier Science BV. SN - 22119264 KW - Antioxidants KW - Fluorescence microscopy KW - Heavy metals KW - Krebs cycle acids KW - Mass spectrometry N2 - Impact of ascorbate biosynthetic inhibitor (lycorine, LYC) on short-term cadmium (Cd) action (1 h, 100 mu M) was studied in the green alga Scenedesmus quadricauda with 1 h or 24 h of LYC co-application. LYC suppressed Cd-induced increase in ascorbic acid and enhanced oxidative symptoms (ROS fluorescence) by modulating activities of antioxidative enzymes after 24 h of addition (decrease in superoxide dismutase, catalase and ascorbate peroxidase activities). Prolonged (24 h) exposure to LYC also affected content of Cd and quantitative changes of thiols (glutathione and phytochelatin 2) while Cd itself revealed interesting responses in algae maintained in the buffer for various time: these data indicate that in the studies involving axenic algal cultures, transfer of cells from sterile to non-sterile conditions modulates metal uptake. Among aliphatic organic acids, a-ketoglutaric, lactic and malic acids were variously influenced by Cd or lycorine, suggesting shift in the respiration and fermentation. It is concluded that ascorbic acid is involved in the protection of cells against Cd-induced ROS over-accumulation and metabolic changes were more expressive in the samples with 24 h co-application of LYC though side impacts of LYC cannot be excluded. DESI-Orbitrap MS for the identification and visualization of lycorine was also successfully tested here for the first time. ER -
KOVÁČIK, Jozef, Bořivoj KLEJDUS, Petr BABULA and Josef HEDBAVNY. Ascorbic acid affects short-term response of Scenedesmus quadricauda to cadmium excess. \textit{Algal Research - Biomass Biofuels and Bioproducts}. Amsterdam: Elsevier Science BV., 2017, vol.~24, JUN 2017, p.~354-359. ISSN~2211-9264. Available from: https://dx.doi.org/10.1016/j.algal.2017.04.026.
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