KOVÁČIK, Jozef, Bořivoj KLEJDUS, Petr BABULA and Josef HEDBAVNY. Ascorbic acid affects short-term response of Scenedesmus quadricauda to cadmium excess. Algal Research - Biomass Biofuels and Bioproducts. Amsterdam: Elsevier Science BV., 2017, vol. 24, JUN 2017, p. 354-359. ISSN 2211-9264. Available from: https://dx.doi.org/10.1016/j.algal.2017.04.026.
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Basic information
Original name Ascorbic acid affects short-term response of Scenedesmus quadricauda to cadmium excess
Authors KOVÁČIK, Jozef (703 Slovakia), Bořivoj KLEJDUS (203 Czech Republic), Petr BABULA (203 Czech Republic, guarantor, belonging to the institution) and Josef HEDBAVNY (203 Czech Republic).
Edition Algal Research - Biomass Biofuels and Bioproducts, Amsterdam, Elsevier Science BV. 2017, 2211-9264.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 3.745
RIV identification code RIV/00216224:14110/17:00097482
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1016/j.algal.2017.04.026
UT WoS 000404864600038
Keywords in English Antioxidants; Fluorescence microscopy; Heavy metals; Krebs cycle acids; Mass spectrometry
Tags EL OK
Tags International impact, Reviewed
Changed by Changed by: Soňa Böhmová, učo 232884. Changed: 8/3/2018 11:12.
Abstract
Impact of ascorbate biosynthetic inhibitor (lycorine, LYC) on short-term cadmium (Cd) action (1 h, 100 mu M) was studied in the green alga Scenedesmus quadricauda with 1 h or 24 h of LYC co-application. LYC suppressed Cd-induced increase in ascorbic acid and enhanced oxidative symptoms (ROS fluorescence) by modulating activities of antioxidative enzymes after 24 h of addition (decrease in superoxide dismutase, catalase and ascorbate peroxidase activities). Prolonged (24 h) exposure to LYC also affected content of Cd and quantitative changes of thiols (glutathione and phytochelatin 2) while Cd itself revealed interesting responses in algae maintained in the buffer for various time: these data indicate that in the studies involving axenic algal cultures, transfer of cells from sterile to non-sterile conditions modulates metal uptake. Among aliphatic organic acids, a-ketoglutaric, lactic and malic acids were variously influenced by Cd or lycorine, suggesting shift in the respiration and fermentation. It is concluded that ascorbic acid is involved in the protection of cells against Cd-induced ROS over-accumulation and metabolic changes were more expressive in the samples with 24 h co-application of LYC though side impacts of LYC cannot be excluded. DESI-Orbitrap MS for the identification and visualization of lycorine was also successfully tested here for the first time.
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