Ascorbic acid affects short-term response of Scenedesmus
quadricauda to cadmium excess

J 2017

Ascorbic acid affects short-term response of Scenedesmus quadricauda to cadmium excess

KOVÁČIK, Jozef, Bořivoj KLEJDUS, Petr BABULA and Josef HEDBAVNY

Basic information

Original name

Ascorbic acid affects short-term response of Scenedesmus quadricauda to cadmium excess

Authors

KOVÁČIK, Jozef (703 Slovakia), Bořivoj KLEJDUS (203 Czech Republic), Petr BABULA (203 Czech Republic, guarantor, belonging to the institution) and Josef HEDBAVNY (203 Czech Republic)

Edition

Algal Research - Biomass Biofuels and Bioproducts, Amsterdam, Elsevier Science BV. 2017, 2211-9264

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.745

RIV identification code

RIV/00216224:14110/17:00097482

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.algal.2017.04.026

UT WoS

000404864600038

Keywords in English

Antioxidants; Fluorescence microscopy; Heavy metals; Krebs cycle acids; Mass spectrometry

Abstract

V originále

Impact of ascorbate biosynthetic inhibitor (lycorine, LYC) on short-term cadmium (Cd) action (1 h, 100 mu M) was studied in the green alga Scenedesmus quadricauda with 1 h or 24 h of LYC co-application. LYC suppressed Cd-induced increase in ascorbic acid and enhanced oxidative symptoms (ROS fluorescence) by modulating activities of antioxidative enzymes after 24 h of addition (decrease in superoxide dismutase, catalase and ascorbate peroxidase activities). Prolonged (24 h) exposure to LYC also affected content of Cd and quantitative changes of thiols (glutathione and phytochelatin 2) while Cd itself revealed interesting responses in algae maintained in the buffer for various time: these data indicate that in the studies involving axenic algal cultures, transfer of cells from sterile to non-sterile conditions modulates metal uptake. Among aliphatic organic acids, a-ketoglutaric, lactic and malic acids were variously influenced by Cd or lycorine, suggesting shift in the respiration and fermentation. It is concluded that ascorbic acid is involved in the protection of cells against Cd-induced ROS over-accumulation and metabolic changes were more expressive in the samples with 24 h co-application of LYC though side impacts of LYC cannot be excluded. DESI-Orbitrap MS for the identification and visualization of lycorine was also successfully tested here for the first time.

Citovat

KOVÁČIK, Jozef, Bořivoj KLEJDUS, Petr BABULA and Josef HEDBAVNY. Ascorbic acid affects short-term response of Scenedesmus quadricauda to cadmium excess. Algal Research - Biomass Biofuels and Bioproducts. Amsterdam: Elsevier Science BV., 2017, vol. 24, JUN 2017, p. 354-359. ISSN 2211-9264. Available from: https://dx.doi.org/10.1016/j.algal.2017.04.026.

@article{1388721,
   author = {Kováčik, Jozef and Klejdus, Bořivoj and Babula, Petr and Hedbavny, Josef},
   article_location = {Amsterdam},
   article_number = {JUN 2017},
   doi = {http://dx.doi.org/10.1016/j.algal.2017.04.026},
   keywords = {Antioxidants; Fluorescence microscopy; Heavy metals; Krebs cycle acids; Mass spectrometry},
   language = {eng},
   issn = {2211-9264},
   journal = {Algal Research - Biomass Biofuels and Bioproducts},
   title = {Ascorbic acid affects short-term response of Scenedesmus quadricauda to cadmium excess},
   volume = {24},
   year = {2017}
}

TY  - JOUR
ID  - 1388721
AU  - Kováčik, Jozef - Klejdus, Bořivoj - Babula, Petr - Hedbavny, Josef
PY  - 2017
TI  - Ascorbic acid affects short-term response of Scenedesmus quadricauda to cadmium excess
JF  - Algal Research - Biomass Biofuels and Bioproducts
VL  - 24
IS  - JUN 2017
SP  - 354-359
EP  - 354-359
PB  - Elsevier Science BV.
SN  - 22119264
KW  - Antioxidants
KW  - Fluorescence microscopy
KW  - Heavy metals
KW  - Krebs cycle acids
KW  - Mass spectrometry
N2  - Impact of ascorbate biosynthetic inhibitor (lycorine, LYC) on short-term cadmium (Cd) action (1 h, 100 mu M) was studied in the green alga Scenedesmus quadricauda with 1 h or 24 h of LYC co-application. LYC suppressed Cd-induced increase in ascorbic acid and enhanced oxidative symptoms (ROS fluorescence) by modulating activities of antioxidative enzymes after 24 h of addition (decrease in superoxide dismutase, catalase and ascorbate peroxidase activities). Prolonged (24 h) exposure to LYC also affected content of Cd and quantitative changes of thiols (glutathione and phytochelatin 2) while Cd itself revealed interesting responses in algae maintained in the buffer for various time: these data indicate that in the studies involving axenic algal cultures, transfer of cells from sterile to non-sterile conditions modulates metal uptake. Among aliphatic organic acids, a-ketoglutaric, lactic and malic acids were variously influenced by Cd or lycorine, suggesting shift in the respiration and fermentation. It is concluded that ascorbic acid is involved in the protection of cells against Cd-induced ROS over-accumulation and metabolic changes were more expressive in the samples with 24 h co-application of LYC though side impacts of LYC cannot be excluded. DESI-Orbitrap MS for the identification and visualization of lycorine was also successfully tested here for the first time.
ER  -

KOVÁČIK, Jozef, Bořivoj KLEJDUS, Petr BABULA and Josef HEDBAVNY. Ascorbic acid affects short-term response of Scenedesmus quadricauda to cadmium excess. \textit{Algal Research - Biomass Biofuels and Bioproducts}. Amsterdam: Elsevier Science BV., 2017, vol.~24, JUN 2017, p.~354-359. ISSN~2211-9264. Available from: https://dx.doi.org/10.1016/j.algal.2017.04.026.

Detailed Information on Publication Record