Hymenoscyphus fraxineus mitovirus 1 naturally disperses through
the airborne inoculum of its host, Hymenoscyphus fraxineus, in
the Czech Republic

J 2017

Hymenoscyphus fraxineus mitovirus 1 naturally disperses through the airborne inoculum of its host, Hymenoscyphus fraxineus, in the Czech Republic

ČERMÁKOVÁ, Vendula, Tomáš KUDLÁČEK, Gabriela ROTKOVÁ, Jiří ROZSYPÁLEK, Leticia BOTELLA et. al.

Basic information

Original name

Hymenoscyphus fraxineus mitovirus 1 naturally disperses through the airborne inoculum of its host, Hymenoscyphus fraxineus, in the Czech Republic

Authors

ČERMÁKOVÁ, Vendula (203 Czech Republic), Tomáš KUDLÁČEK (203 Czech Republic), Gabriela ROTKOVÁ (203 Czech Republic, belonging to the institution), Jiří ROZSYPÁLEK (203 Czech Republic) and Leticia BOTELLA (724 Spain)

Edition

Biocontrol Science and Technology, Abingdon, TAYLOR & FRANCIS LTD, 2017, 0958-3157

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.918

RIV identification code

RIV/00216224:14310/17:00097502

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1080/09583157.2017.1368455

UT WoS

000410578100007

Keywords in English

Ash dieback; mitoviruses; sexual transmission; qPCR; Chalara fraxinea; spore trap; aerospora

Abstract

V originále

We investigated the ability of Hymenoscyphus fraxineus mitovirus 1 (HfMV1) to spread in nature through the airborne inoculum of its fungal host, the devastating Hymenoscyphus fraxineus. Eight rotating-arm spore traps were installed to collect the airborne spores (presumably ascospores) of H. fraxineus in four ecologically different ash forests in the Czech Republic. Spore trap samples were subjected to direct DNA and RNA extractions and subsequent qPCR and mitovirus-specific reverse transcription (RT) qPCR for detection and quantification of H. fraxineus and HfMV1, respectively. The prevalence of HfMV1 was confirmed in 57% of H. fraxineus sampled inocula, showing that its occurrence is significantly correlated with its fungal host H. fraxineus. Furthermore, the ratio of mitovirus carrying spores was shown to be constant at any level of H. fraxineus infection pressure independently of the studied locality. Temperatures of 19–21°C at the time of spore trapping correlated with the highest levels of both HfMV1 and H. fraxineus. Our data confirm H. fraxineus airborne inoculum as an efficient transport means for HfMV1 in nature. Moreover, this research uses a new technology to directly study a mycovirus through its host aerospora.

Citovat

ČERMÁKOVÁ, Vendula, Tomáš KUDLÁČEK, Gabriela ROTKOVÁ, Jiří ROZSYPÁLEK and Leticia BOTELLA. Hymenoscyphus fraxineus mitovirus 1 naturally disperses through the airborne inoculum of its host, Hymenoscyphus fraxineus, in the Czech Republic. Biocontrol Science and Technology. Abingdon: TAYLOR & FRANCIS LTD, 2017, vol. 27, No 8, p. 992-1008. ISSN 0958-3157. Available from: https://dx.doi.org/10.1080/09583157.2017.1368455.

@article{1389079,
   author = {Čermáková, Vendula and Kudláček, Tomáš and Rotková, Gabriela and Rozsypálek, Jiří and Botella, Leticia},
   article_location = {Abingdon},
   article_number = {8},
   doi = {http://dx.doi.org/10.1080/09583157.2017.1368455},
   keywords = {Ash dieback; mitoviruses; sexual transmission; qPCR; Chalara fraxinea; spore trap; aerospora},
   language = {eng},
   issn = {0958-3157},
   journal = {Biocontrol Science and Technology},
   title = {Hymenoscyphus fraxineus mitovirus 1 naturally disperses through the airborne inoculum of its host, Hymenoscyphus fraxineus, in the Czech Republic},
   volume = {27},
   year = {2017}
}

TY  - JOUR
ID  - 1389079
AU  - Čermáková, Vendula - Kudláček, Tomáš - Rotková, Gabriela - Rozsypálek, Jiří - Botella, Leticia
PY  - 2017
TI  - Hymenoscyphus fraxineus mitovirus 1 naturally disperses through the airborne inoculum of its host, Hymenoscyphus fraxineus, in the Czech Republic
JF  - Biocontrol Science and Technology
VL  - 27
IS  - 8
SP  - 992-1008
EP  - 992-1008
PB  - TAYLOR & FRANCIS LTD
SN  - 09583157
KW  - Ash dieback
KW  - mitoviruses
KW  - sexual transmission
KW  - qPCR
KW  - Chalara fraxinea
KW  - spore trap
KW  - aerospora
N2  - We investigated the ability of Hymenoscyphus fraxineus mitovirus 1 (HfMV1) to spread in nature through the airborne inoculum of its fungal host, the devastating Hymenoscyphus fraxineus. Eight rotating-arm spore traps were installed to collect the airborne spores (presumably ascospores) of H. fraxineus in four ecologically different ash forests in the Czech Republic. Spore trap samples were subjected to direct DNA and RNA extractions and subsequent qPCR and mitovirus-specific reverse transcription (RT) qPCR for detection and quantification of H. fraxineus and HfMV1, respectively. The prevalence of HfMV1 was confirmed in 57% of H. fraxineus sampled inocula, showing that its occurrence is significantly correlated with its fungal host H. fraxineus. Furthermore, the ratio of mitovirus carrying spores was shown to be constant at any level of H. fraxineus infection pressure independently of the studied locality. Temperatures of 19–21°C at the time of spore trapping correlated with the highest levels of both HfMV1 and H. fraxineus. Our data confirm H. fraxineus airborne inoculum as an efficient transport means for HfMV1 in nature. Moreover, this research uses a new technology to directly study a mycovirus through its host aerospora.
ER  -

ČERMÁKOVÁ, Vendula, Tomáš KUDLÁČEK, Gabriela ROTKOVÁ, Jiří ROZSYPÁLEK and Leticia BOTELLA. Hymenoscyphus fraxineus mitovirus 1 naturally disperses through the airborne inoculum of its host, Hymenoscyphus fraxineus, in the Czech Republic. \textit{Biocontrol Science and Technology}. Abingdon: TAYLOR \&{} FRANCIS LTD, 2017, vol.~27, No~8, p.~992-1008. ISSN~0958-3157. Available from: https://dx.doi.org/10.1080/09583157.2017.1368455.

Detailed Information on Publication Record