Long-term impact of cadmium shows little damage in Scenedesmus
acutiformis cultures

J 2017

Long-term impact of cadmium shows little damage in Scenedesmus acutiformis cultures

KOVACIK, Jozef, Petr BABULA, Viera PETERKOVA and Josef HEDBAVNY

Basic information

Original name

Long-term impact of cadmium shows little damage in Scenedesmus acutiformis cultures

Authors

KOVACIK, Jozef (703 Slovakia), Petr BABULA (203 Czech Republic, guarantor, belonging to the institution), Viera PETERKOVA (703 Slovakia) and Josef HEDBAVNY (203 Czech Republic)

Edition

Algal Research - Biomass Biofuels and Bioproducts, Amsterdam, Elsevier Science BV. 2017, 2211-9264

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.745

RIV identification code

RIV/00216224:14110/17:00097503

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.algal.2017.04.029

UT WoS

000406305900022

Keywords in English

Antioxidants; Axenic culture; Bioaccumulation; Fluorescence microscopy; Heavy metals

Abstract

V originále

Long-term (30 days) responses of green alga Scenedesmus acutiformis to cadmium (Cd) excess (1 or 10 mu M) were studied under in vitro heterotrophic conditions (Milieu Bristol medium containing glucose and casein). No extensive changes of morphology were observed but Cd slightly depleted chlorophyll autofluorescence and some mineral content (K, Ca, and Mg). However, Cd (mainly at 10 mu M) stimulated increase in biomass and accumulation of ascorbic acid, non-protein thiols, and phenols. Signs of oxidative stress (elevation of ROS, depletion of nitric oxide and viability, changes in the activities of SOD, CAT and GR) were also observed. Total Cd reached only 17.3 and 45.2 mu g/g DW at 1 or 10 mu M Cd but bioaccumulation factor (BAF) was comparable with other species and cultivation/exposure conditions (BAF similar to 40 at 10 mu M Cd) indicating that rather inorganic compounds than glucose or casein affect Cd accumulation. Short-term (24 h) exposure of algae to identical Cd doses in HEPES buffer revealed higher absolute Cd content in comparison with 30 days of cultivation (8-and 17fold) and comparison with previous studies indicates that S. acutiformis is a better cadmium accumulator. The extensive use of fluorescence microscopy for oxidative stress detection in algae is also carefully discussed.

Citovat

KOVACIK, Jozef, Petr BABULA, Viera PETERKOVA and Josef HEDBAVNY. Long-term impact of cadmium shows little damage in Scenedesmus acutiformis cultures. Algal Research - Biomass Biofuels and Bioproducts. Amsterdam: Elsevier Science BV., 2017, vol. 25, JUL 2017, p. 184-190. ISSN 2211-9264. Available from: https://dx.doi.org/10.1016/j.algal.2017.04.029.

@article{1389080,
   author = {Kovacik, Jozef and Babula, Petr and Peterkova, Viera and Hedbavny, Josef},
   article_location = {Amsterdam},
   article_number = {JUL 2017},
   doi = {http://dx.doi.org/10.1016/j.algal.2017.04.029},
   keywords = {Antioxidants; Axenic culture; Bioaccumulation; Fluorescence microscopy; Heavy metals},
   language = {eng},
   issn = {2211-9264},
   journal = {Algal Research - Biomass Biofuels and Bioproducts},
   title = {Long-term impact of cadmium shows little damage in Scenedesmus acutiformis cultures},
   volume = {25},
   year = {2017}
}

TY  - JOUR
ID  - 1389080
AU  - Kovacik, Jozef - Babula, Petr - Peterkova, Viera - Hedbavny, Josef
PY  - 2017
TI  - Long-term impact of cadmium shows little damage in Scenedesmus acutiformis cultures
JF  - Algal Research - Biomass Biofuels and Bioproducts
VL  - 25
IS  - JUL 2017
SP  - 184-190
EP  - 184-190
PB  - Elsevier Science BV.
SN  - 22119264
KW  - Antioxidants
KW  - Axenic culture
KW  - Bioaccumulation
KW  - Fluorescence microscopy
KW  - Heavy metals
N2  - Long-term (30 days) responses of green alga Scenedesmus acutiformis to cadmium (Cd) excess (1 or 10 mu M) were studied under in vitro heterotrophic conditions (Milieu Bristol medium containing glucose and casein). No extensive changes of morphology were observed but Cd slightly depleted chlorophyll autofluorescence and some mineral content (K, Ca, and Mg). However, Cd (mainly at 10 mu M) stimulated increase in biomass and accumulation of ascorbic acid, non-protein thiols, and phenols. Signs of oxidative stress (elevation of ROS, depletion of nitric oxide and viability, changes in the activities of SOD, CAT and GR) were also observed. Total Cd reached only 17.3 and 45.2 mu g/g DW at 1 or 10 mu M Cd but bioaccumulation factor (BAF) was comparable with other species and cultivation/exposure conditions (BAF similar to 40 at 10 mu M Cd) indicating that rather inorganic compounds than glucose or casein affect Cd accumulation. Short-term (24 h) exposure of algae to identical Cd doses in HEPES buffer revealed higher absolute Cd content in comparison with 30 days of cultivation (8-and 17fold) and comparison with previous studies indicates that S. acutiformis is a better cadmium accumulator. The extensive use of fluorescence microscopy for oxidative stress detection in algae is also carefully discussed.
ER  -

KOVACIK, Jozef, Petr BABULA, Viera PETERKOVA and Josef HEDBAVNY. Long-term impact of cadmium shows little damage in Scenedesmus acutiformis cultures. \textit{Algal Research - Biomass Biofuels and Bioproducts}. Amsterdam: Elsevier Science BV., 2017, vol.~25, JUL 2017, p.~184-190. ISSN~2211-9264. Available from: https://dx.doi.org/10.1016/j.algal.2017.04.029.

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