Two highly divergent lineages of exfoliative toxin B-encoding
plasmids revealed in impetigo strains of Staphylococcus aureus

J 2017

Two highly divergent lineages of exfoliative toxin B-encoding plasmids revealed in impetigo strains of Staphylococcus aureus

BOTKA, Tibor, Vladislava RŮŽIČKOVÁ, Karla SVOBODOVÁ, Roman PANTŮČEK, Petr PETRÁŠ et. al.

Basic information

Original name

Two highly divergent lineages of exfoliative toxin B-encoding plasmids revealed in impetigo strains of Staphylococcus aureus

Authors

BOTKA, Tibor (203 Czech Republic, belonging to the institution), Vladislava RŮŽIČKOVÁ (203 Czech Republic, guarantor, belonging to the institution), Karla SVOBODOVÁ (203 Czech Republic, belonging to the institution), Roman PANTŮČEK (203 Czech Republic, belonging to the institution), Petr PETRÁŠ (203 Czech Republic), Darina ČEJKOVÁ (203 Czech Republic) and Jiří DOŠKAŘ (203 Czech Republic, belonging to the institution)

Edition

International Journal of Medical Microbiology, Jena, ELSEVIER GMBH, URBAN & FISCHER VERLAG, 2017, 1438-4221

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.298

RIV identification code

RIV/00216224:14310/17:00094338

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.ijmm.2017.05.005

UT WoS

000412041100002

Keywords (in Czech)

Staphylococcus aureus; exfoliativní toxin B; ETB plazmidy; bulózní impetigo; fylogeneze

Keywords in English

Staphylococcus aureus; Exfoliative toxin B; ETB plasmids; Bullous impetigo; Phylogeny

Abstract

V originále

Exfoliative toxin B (ETB) encoded by some large plasmids plays a crucial role in epidermolytic diseases caused by Staphylococcus aureus. We have found as yet unknown types of etb gene-positive plasmids isolated from a set of impetigo strains implicated in outbreaks of pemphigus neonatorum in Czech maternity hospitals. Plasmids from the strains of clonal complex CC121 were related to archetypal plasmid pETBTY4. Sharing a 33-kb core sequence including virulence genes for ETB, EDIN C, and lantibiotics, they were assigned to a stand-alone lineage, named pETBTY4-based plasmids. Differing from each other in the content of variable DNA regions, they formed four sequence types. In addition to them, a novel unique plasmid pETB608 isolated from a strain of ST130 was described. Carrying conjugative cluster genes, as well as new variants of etb and edinA genes, pETB608 could be regarded as a source of a new lineage of ETB plasmids. We have designed a helpful detection assay, which facilitates the precise identification of the all described types of ETB plasmids.

Links

MUNI/A/0967/2015, interní kód MU

Name: Podpora výzkumné činnosti studentů molekulární biologie a genetiky 4 (Acronym: MBG4)

Investor: Masaryk University, Category A

QJ1510216, research and development project

Name: Fágová terapie infekcí vyvolaných Staphylococcus aureus v chovech hospodářských zvířat (Acronym: StafyFag)

Investor: Ministry of Agriculture of the CR

Files attached

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Citovat

BOTKA, Tibor, Vladislava RŮŽIČKOVÁ, Karla SVOBODOVÁ, Roman PANTŮČEK, Petr PETRÁŠ, Darina ČEJKOVÁ and Jiří DOŠKAŘ. Two highly divergent lineages of exfoliative toxin B-encoding plasmids revealed in impetigo strains of Staphylococcus aureus. International Journal of Medical Microbiology. Jena: ELSEVIER GMBH, URBAN & FISCHER VERLAG, 2017, vol. 307, No 6, p. 291-296. ISSN 1438-4221. Available from: https://dx.doi.org/10.1016/j.ijmm.2017.05.005.

@article{1389107,
   author = {Botka, Tibor and Růžičková, Vladislava and Svobodová, Karla and Pantůček, Roman and Petráš, Petr and Čejková, Darina and Doškař, Jiří},
   article_location = {Jena},
   article_number = {6},
   doi = {http://dx.doi.org/10.1016/j.ijmm.2017.05.005},
   keywords = {Staphylococcus aureus; Exfoliative toxin B; ETB plasmids; Bullous impetigo; Phylogeny},
   language = {eng},
   issn = {1438-4221},
   journal = {International Journal of Medical Microbiology},
   title = {Two highly divergent lineages of exfoliative toxin B-encoding plasmids revealed in impetigo strains of Staphylococcus aureus},
   url = {http://www.sciencedirect.com/science/article/pii/S1438422117301157},
   volume = {307},
   year = {2017}
}

TY  - JOUR
ID  - 1389107
AU  - Botka, Tibor - Růžičková, Vladislava - Svobodová, Karla - Pantůček, Roman - Petráš, Petr - Čejková, Darina - Doškař, Jiří
PY  - 2017
TI  - Two highly divergent lineages of exfoliative toxin B-encoding plasmids revealed in impetigo strains of Staphylococcus aureus
JF  - International Journal of Medical Microbiology
VL  - 307
IS  - 6
SP  - 291-296
EP  - 291-296
PB  - ELSEVIER GMBH, URBAN & FISCHER VERLAG
SN  - 14384221
KW  - Staphylococcus aureus
KW  - Exfoliative toxin B
KW  - ETB plasmids
KW  - Bullous impetigo
KW  - Phylogeny
UR  - http://www.sciencedirect.com/science/article/pii/S1438422117301157
L2  - http://www.sciencedirect.com/science/article/pii/S1438422117301157
N2  - Exfoliative toxin B (ETB) encoded by some large plasmids plays a crucial role in epidermolytic diseases caused by Staphylococcus aureus. We have found as yet unknown types of etb gene-positive plasmids isolated from a set of impetigo strains implicated in outbreaks of pemphigus neonatorum in Czech maternity hospitals. Plasmids from the strains of clonal complex CC121 were related to archetypal plasmid pETBTY4. Sharing a 33-kb core sequence including virulence genes for ETB, EDIN C, and lantibiotics, they were assigned to a stand-alone lineage, named pETBTY4-based plasmids. Differing from each other in the content of variable DNA regions, they formed four sequence types. In addition to them, a novel unique plasmid pETB608 isolated from a strain of ST130 was described. Carrying conjugative cluster genes, as well as new variants of etb and edinA genes, pETB608 could be regarded as a source of a new lineage of ETB plasmids. We have designed a helpful detection assay, which facilitates the precise identification of the all described types of ETB plasmids.
ER  -

BOTKA, Tibor, Vladislava RŮŽIČKOVÁ, Karla SVOBODOVÁ, Roman PANTŮČEK, Petr PETRÁŠ, Darina ČEJKOVÁ and Jiří DOŠKAŘ. Two highly divergent lineages of exfoliative toxin B-encoding plasmids revealed in impetigo strains of Staphylococcus aureus. \textit{International Journal of Medical Microbiology}. Jena: ELSEVIER GMBH, URBAN \&{} FISCHER VERLAG, 2017, vol.~307, No~6, p.~291-296. ISSN~1438-4221. Available from: https://dx.doi.org/10.1016/j.ijmm.2017.05.005.

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