Podrobný výpis o publikaci

CIPAK, A, L MRAKOVCIC, Milan ČÍŽ, Antonín LOJEK, B MIHAYLOVA, I GOSHEV, M JAGANJAC, M CINDRIC, S SITIC, M MARGARITONI, G WAEG, M BALIC a N ZARKOVIC. Growth suppression of human breast carcinoma stem cells by lipid peroxidation product 4-hydroxy-2-nonenal and hydroxyl radical-modified collagen. Acta Biochimica Polonica. WARSAW: Polish Academy of Sciences, Committee of Biochemistry, 2010, roč. 57, č. 2, s. 165-171. ISSN 0001-527X.

Další formáty: BibTeX LaTeX RIS

TY  - JOUR
ID  - 1389170
AU  - Cipak, A - Mrakovcic, L - Číž, Milan - Lojek, Antonín - Mihaylova, B - Goshev, I - Jaganjac, M - Cindric, M - Sitic, S - Margaritoni, M - Waeg, G - Balic, M - Zarkovic, N
PY  - 2010
TI  - Growth suppression of human breast carcinoma stem cells by lipid peroxidation product 4-hydroxy-2-nonenal and hydroxyl radical-modified collagen
JF  - Acta Biochimica Polonica
VL  - 57
IS  - 2
SP  - 165-171
EP  - 165-171
PB  - Polish Academy of Sciences, Committee of Biochemistry
SN  - 0001527X
KW  - SUM159
KW  - breast cancer stem cells
KW  - 4-hydroxynonenal
KW  - extracellular matrix
KW  - collagen
KW  - oxidative homeostasis
N2  - Breast cancer is a leading cause of mortality and morbidity in women, mostly due to high metastatic capacity of mammary carcinoma cells. It has been revealed recently that metastases of breast cancer comprise a fraction of specific stem-like cells, denoted as cancer stem cells (CSCs). Breast CSCs, expressing specific surface markers CD44(+)CD24(-/low)ESA(+) usually disseminate in the bone marrow, being able to spread further and cause late metastases. The fundamental factor influencing the growth of CSCs is the microenvironment, especially the interaction of CSCs with extracellular matrix (ECM). The structure and function of ECM proteins, such as the dominating ECM protein collagen, is influenced not only by cancer cells but also by various cancer treatments. Since surgery, radio and chemotherapy are associated with oxidative stress we analyzed the growth of breast cancer CD44(+)CD24(-/low)ESA(+) cell line SUM159 cultured on collagen matrix in vitro, using either native collagen or the one modified by hydroxyl radical. While native collagen supported the growth of CSCs, oxidatively modified one was not supportive. The SUM159 cell cultures were further exposed to a supraphysiological (35 mu M) dose of the major bioactive lipid peroxidation product 4-hydroxynonenal (HNE), a well known as "second messenger of free radicals", which has a strong affinity to bind to proteins and acts as a cytotoxic or as growth regulating signaling molecule. Native collagen, but not oxidised, abolished cytotoxicity of HNE, while oxidized collagen did not reduce cytotoxicity of HNE at all. These preliminary findings indicate that beside direct cytotoxic effects of anticancer therapies consequential oxidative stress and lipid peroxidation modify the microenvironment of CSCs influencing oxidative homeostasis that could additionally act against cancer.
ER  -

Základní údaje
Originální název	Growth suppression of human breast carcinoma stem cells by lipid peroxidation product 4-hydroxy-2-nonenal and hydroxyl radical-modified collagen
Autoři	CIPAK, A, L MRAKOVCIC, Milan ČÍŽ, Antonín LOJEK, B MIHAYLOVA, I GOSHEV, M JAGANJAC, M CINDRIC, S SITIC, M MARGARITONI, G WAEG, M BALIC a N ZARKOVIC.
Vydání	Acta Biochimica Polonica, WARSAW, Polish Academy of Sciences, Committee of Biochemistry, 2010, 0001-527X.

Další údaje
Originální jazyk	angličtina
Typ výsledku	Článek v odborném periodiku
Utajení	není předmětem státního či obchodního tajemství
Impakt faktor	Impact factor: 1.234
UT WoS	000279416900006
Klíčová slova anglicky	SUM159; breast cancer stem cells; 4-hydroxynonenal; extracellular matrix; collagen; oxidative homeostasis
Změnil	Změnil: doc. RNDr. Milan Číž, Ph.D., učo 31503. Změněno: 12. 9. 2017 12:40.

Anotace
Breast cancer is a leading cause of mortality and morbidity in women, mostly due to high metastatic capacity of mammary carcinoma cells. It has been revealed recently that metastases of breast cancer comprise a fraction of specific stem-like cells, denoted as cancer stem cells (CSCs). Breast CSCs, expressing specific surface markers CD44(+)CD24(-/low)ESA(+) usually disseminate in the bone marrow, being able to spread further and cause late metastases. The fundamental factor influencing the growth of CSCs is the microenvironment, especially the interaction of CSCs with extracellular matrix (ECM). The structure and function of ECM proteins, such as the dominating ECM protein collagen, is influenced not only by cancer cells but also by various cancer treatments. Since surgery, radio and chemotherapy are associated with oxidative stress we analyzed the growth of breast cancer CD44(+)CD24(-/low)ESA(+) cell line SUM159 cultured on collagen matrix in vitro, using either native collagen or the one modified by hydroxyl radical. While native collagen supported the growth of CSCs, oxidatively modified one was not supportive. The SUM159 cell cultures were further exposed to a supraphysiological (35 mu M) dose of the major bioactive lipid peroxidation product 4-hydroxynonenal (HNE), a well known as "second messenger of free radicals", which has a strong affinity to bind to proteins and acts as a cytotoxic or as growth regulating signaling molecule. Native collagen, but not oxidised, abolished cytotoxicity of HNE, while oxidized collagen did not reduce cytotoxicity of HNE at all. These preliminary findings indicate that beside direct cytotoxic effects of anticancer therapies consequential oxidative stress and lipid peroxidation modify the microenvironment of CSCs influencing oxidative homeostasis that could additionally act against cancer.

Anotace

Breast cancer is a leading cause of mortality and morbidity in women, mostly due to high metastatic capacity of mammary carcinoma cells. It has been revealed recently that metastases of breast cancer comprise a fraction of specific stem-like cells, denoted as cancer stem cells (CSCs). Breast CSCs, expressing specific surface markers CD44(+)CD24(-/low)ESA(+) usually disseminate in the bone marrow, being able to spread further and cause late metastases. The fundamental factor influencing the growth of CSCs is the microenvironment, especially the interaction of CSCs with extracellular matrix (ECM). The structure and function of ECM proteins, such as the dominating ECM protein collagen, is influenced not only by cancer cells but also by various cancer treatments. Since surgery, radio and chemotherapy are associated with oxidative stress we analyzed the growth of breast cancer CD44(+)CD24(-/low)ESA(+) cell line SUM159 cultured on collagen matrix in vitro, using either native collagen or the one modified by hydroxyl radical. While native collagen supported the growth of CSCs, oxidatively modified one was not supportive. The SUM159 cell cultures were further exposed to a supraphysiological (35 mu M) dose of the major bioactive lipid peroxidation product 4-hydroxynonenal (HNE), a well known as "second messenger of free radicals", which has a strong affinity to bind to proteins and acts as a cytotoxic or as growth regulating signaling molecule. Native collagen, but not oxidised, abolished cytotoxicity of HNE, while oxidized collagen did not reduce cytotoxicity of HNE at all. These preliminary findings indicate that beside direct cytotoxic effects of anticancer therapies consequential oxidative stress and lipid peroxidation modify the microenvironment of CSCs influencing oxidative homeostasis that could additionally act against cancer.