Tyrosine Kinase Expressed in Hepatocellular Carcinoma, TEC,
Controls Pluripotency and Early Cell Fate Decisions of Human
Pluripotent Stem Cells via Regulation of Fibroblast Growth
Factor-2 Secretion

J 2017

Tyrosine Kinase Expressed in Hepatocellular Carcinoma, TEC, Controls Pluripotency and Early Cell Fate Decisions of Human Pluripotent Stem Cells via Regulation of Fibroblast Growth Factor-2 Secretion

VÁŇOVÁ, Tereza, Žaneta KONEČNÁ, Zuzana ZBOŇÁKOVÁ, Giuseppe LA VENUTA, Karolína ZOUFALOVÁ et. al.

Basic information

Original name

Tyrosine Kinase Expressed in Hepatocellular Carcinoma, TEC, Controls Pluripotency and Early Cell Fate Decisions of Human Pluripotent Stem Cells via Regulation of Fibroblast Growth Factor-2 Secretion

Authors

VÁŇOVÁ, Tereza (203 Czech Republic, belonging to the institution), Žaneta KONEČNÁ (203 Czech Republic, belonging to the institution), Zuzana ZBOŇÁKOVÁ (703 Slovakia, belonging to the institution), Giuseppe LA VENUTA (276 Germany), Karolína ZOUFALOVÁ (203 Czech Republic, belonging to the institution), Šárka JELÍNKOVÁ (203 Czech Republic, belonging to the institution), Miroslav VAŘECHA (203 Czech Republic, belonging to the institution), Vladimír ROTREKL (203 Czech Republic, belonging to the institution), Pavel KREJČÍ (203 Czech Republic, belonging to the institution), Walter NICKEL (276 Germany), Petr DVOŘÁK (203 Czech Republic, guarantor, belonging to the institution) and Michaela BOSÁKOVÁ (203 Czech Republic, belonging to the institution)

Edition

Stem Cells, UNITED STATES, WILEY-BLACKWELL, 2017, 1066-5099

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 5.587

RIV identification code

RIV/00216224:14110/17:00094930

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1002/stem.2660

UT WoS

000408334300004

Keywords in English

Tyrosine kinase expressed in hepatocellular carcinoma; Fibroblast growth factor; Fibroblast growth factor 2; Pluripotent stem cells; Embryonic stem cells; Neural differentiation; Cardiac differentiation

Abstract

V originále

Human pluripotent stem cells (hPSC) require signaling provided by fibroblast growth factor (FGF) receptors. This can be initiated by the recombinant FGF2 ligand supplied exogenously, but hPSC further support their niche by secretion of endogenous FGF2. In this study, we describe a role of tyrosine kinase expressed in hepatocellular carcinoma (TEC) kinase in this process. We show that TEC-mediated FGF2 secretion is essential for hPSC self-renewal, and its lack mediates specific differentiation. Following both short hairpin RNA- and small interfering RNA-mediated TEC knockdown, hPSC secretes less FGF2. This impairs hPSC proliferation that can be rescued by increasing amounts of recombinant FGF2. TEC downregulation further leads to a lower expression of the pluripotency markers, an improved priming towards neuroectodermal lineage, and a failure to develop cardiac mesoderm. Our data thus demonstrate that TEC is yet another regulator of FGF2-mediated hPSC pluripotency and differentiation.

Links

GA15-23033S, research and development project

Name: Úloha sekrece FGF2 ve fyziologii lidských pluripotentních kmenových buněk

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

NV15-33232A, research and development project

Name: Identifikace nových možností léčby achondroplásie prostřednictvím analýzy interakce FGFR3 a adaptérového proteinu Frs2

Citovat

VÁŇOVÁ, Tereza, Žaneta KONEČNÁ, Zuzana ZBOŇÁKOVÁ, Giuseppe LA VENUTA, Karolína ZOUFALOVÁ, Šárka JELÍNKOVÁ, Miroslav VAŘECHA, Vladimír ROTREKL, Pavel KREJČÍ, Walter NICKEL, Petr DVOŘÁK and Michaela BOSÁKOVÁ. Tyrosine Kinase Expressed in Hepatocellular Carcinoma, TEC, Controls Pluripotency and Early Cell Fate Decisions of Human Pluripotent Stem Cells via Regulation of Fibroblast Growth Factor-2 Secretion. Stem Cells. UNITED STATES: WILEY-BLACKWELL, 2017, vol. 35, No 9, p. 2050-2059. ISSN 1066-5099. Available from: https://dx.doi.org/10.1002/stem.2660.

@article{1389283,
   author = {Váňová, Tereza and Konečná, Žaneta and Zboňáková, Zuzana and La Venuta, Giuseppe and Zoufalová, Karolína and Jelínková, Šárka and Vařecha, Miroslav and Rotrekl, Vladimír and Krejčí, Pavel and Nickel, Walter and Dvořák, Petr and Bosáková, Michaela},
   article_location = {UNITED STATES},
   article_number = {9},
   doi = {http://dx.doi.org/10.1002/stem.2660},
   keywords = {Tyrosine kinase expressed in hepatocellular carcinoma; Fibroblast growth factor; Fibroblast growth factor 2; Pluripotent stem cells; Embryonic stem cells; Neural differentiation; Cardiac differentiation},
   language = {eng},
   issn = {1066-5099},
   journal = {Stem Cells},
   title = {Tyrosine Kinase Expressed in Hepatocellular Carcinoma, TEC, Controls Pluripotency and Early Cell Fate Decisions of Human Pluripotent Stem Cells via Regulation of Fibroblast Growth Factor-2 Secretion},
   volume = {35},
   year = {2017}
}

TY  - JOUR
ID  - 1389283
AU  - Váňová, Tereza - Konečná, Žaneta - Zboňáková, Zuzana - La Venuta, Giuseppe - Zoufalová, Karolína - Jelínková, Šárka - Vařecha, Miroslav - Rotrekl, Vladimír - Krejčí, Pavel - Nickel, Walter - Dvořák, Petr - Bosáková, Michaela
PY  - 2017
TI  - Tyrosine Kinase Expressed in Hepatocellular Carcinoma, TEC, Controls Pluripotency and Early Cell Fate Decisions of Human Pluripotent Stem Cells via Regulation of Fibroblast Growth Factor-2 Secretion
JF  - Stem Cells
VL  - 35
IS  - 9
SP  - 2050-2059
EP  - 2050-2059
PB  - WILEY-BLACKWELL
SN  - 10665099
KW  - Tyrosine kinase expressed in hepatocellular carcinoma
KW  - Fibroblast growth factor
KW  - Fibroblast growth factor 2
KW  - Pluripotent stem cells
KW  - Embryonic stem cells
KW  - Neural differentiation
KW  - Cardiac differentiation
N2  - Human pluripotent stem cells (hPSC) require signaling provided by fibroblast growth factor (FGF) receptors. This can be initiated by the recombinant FGF2 ligand supplied exogenously, but hPSC further support their niche by secretion of endogenous FGF2. In this study, we describe a role of tyrosine kinase expressed in hepatocellular carcinoma (TEC) kinase in this process. We show that TEC-mediated FGF2 secretion is essential for hPSC self-renewal, and its lack mediates specific differentiation. Following both short hairpin RNA- and small interfering RNA-mediated TEC knockdown, hPSC secretes less FGF2. This impairs hPSC proliferation that can be rescued by increasing amounts of recombinant FGF2. TEC downregulation further leads to a lower expression of the pluripotency markers, an improved priming towards neuroectodermal lineage, and a failure to develop cardiac mesoderm. Our data thus demonstrate that TEC is yet another regulator of FGF2-mediated hPSC pluripotency and differentiation.
ER  -

VÁŇOVÁ, Tereza, Žaneta KONEČNÁ, Zuzana ZBOŇÁKOVÁ, Giuseppe LA VENUTA, Karolína ZOUFALOVÁ, Šárka JELÍNKOVÁ, Miroslav VAŘECHA, Vladimír ROTREKL, Pavel KREJČÍ, Walter NICKEL, Petr DVOŘÁK and Michaela BOSÁKOVÁ. Tyrosine Kinase Expressed in Hepatocellular Carcinoma, TEC, Controls Pluripotency and Early Cell Fate Decisions of Human Pluripotent Stem Cells via Regulation of Fibroblast Growth Factor-2 Secretion. \textit{Stem Cells}. UNITED STATES: WILEY-BLACKWELL, 2017, vol.~35, No~9, p.~2050-2059. ISSN~1066-5099. Available from: https://dx.doi.org/10.1002/stem.2660.

Detailed Information on Publication Record