Complete genome sequences of two strains of Treponema pallidum
subsp. pertenue from Ghana, Africa: Identical genome sequences
in samples isolated more than 7 years apart

J 2017

Complete genome sequences of two strains of Treponema pallidum subsp. pertenue from Ghana, Africa: Identical genome sequences in samples isolated more than 7 years apart

STROUHAL, Michal, Lenka PAŠTĚKOVÁ, Pavla HAVLÍČKOVÁ, Paolo TENTI, Darina ČEJKOVÁ et. al.

Basic information

Original name

Complete genome sequences of two strains of Treponema pallidum subsp. pertenue from Ghana, Africa: Identical genome sequences in samples isolated more than 7 years apart

Authors

STROUHAL, Michal (203 Czech Republic, belonging to the institution), Lenka PAŠTĚKOVÁ (203 Czech Republic, belonging to the institution), Pavla HAVLÍČKOVÁ (203 Czech Republic, belonging to the institution), Paolo TENTI (380 Italy, belonging to the institution), Darina ČEJKOVÁ (203 Czech Republic), Ivan RYCHLÍK (203 Czech Republic), Sylvia BRUISTEN (528 Netherlands) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)

Edition

PLOS Neglected Tropical Diseases, San Francisco, Public Library of Science, 2017, 1935-2735

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30309 Tropical medicine

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.367

RIV identification code

RIV/00216224:14110/17:00094932

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1371/journal.pntd.0005894

UT WoS

000412142800040

Keywords in English

Treponema pallidum subsp pertenue

Abstract

V originále

Treponema pallidum subsp. pertenue (TPE) is the causative agent of yaws, a multi-stage disease, endemic in tropical regions of Africa, Asia, Oceania, and South America. To date, four TPE strains have been completely sequenced including three TPE strains of human origin (Samoa D, CDC-2, and Gauthier) and one TPE strain (Fribourg-Blanc) isolated from a baboon. All TPE strains are highly similar to T. pallidum subsp. pallidum (TPA) strains. The mutation rate in syphilis and related treponemes has not been experimentally determined yet. Complete genomes of two TPE strains, CDC 2575 and Ghana-051, that infected patients in Ghana and were isolated in 1980 and 1988, respectively, were sequenced and analyzed. Both strains had identical consensus genome nucleotide sequences raising the question whether TPE CDC 2575 and Ghana-051 represent two different strains. Several lines of evidence support the fact that both strains represent independent samples including regions showing intrastrain heterogeneity (13 and 5 intrastrain heterogeneous sites in TPE Ghana-051 and TPE CDC 2575, respectively). Four of these heterogeneous sites were found in both genomes but the frequency of alternative alleles differed. The identical consensus genome sequences were used to estimate the upper limit of the yaws treponeme evolution rate, which was 4.1 x 10-10 nucleotide changes per site per generation. The estimated upper limit for the mutation rate of TPE was slightly lower than the mutation rate of E. coli, which was determined during a long-term experiment. Given the known diversity between TPA and TPE genomes and the assumption that both TPA and TPE have a similar mutation rate, the most recent common ancestor of syphilis and yaws treponemes appears to be more than ten thousand years old and likely even older.

Links

GA17-25455S, research and development project

Name: Studium genomů patogenních treponem na základě analýzy jednotlivých buněk

Investor: Czech Science Foundation

GJ17-25589Y, research and development project

Name: Kmeny způsobující syfilis: jsou geneticky odlišné kmeny odlišné i v průběhu experimentální infekce?

Investor: Czech Science Foundation

ROZV/24/LF/2016, interní kód MU

Name: LF - Příspěvek IP 2016

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

STROUHAL, Michal, Lenka PAŠTĚKOVÁ, Pavla HAVLÍČKOVÁ, Paolo TENTI, Darina ČEJKOVÁ, Ivan RYCHLÍK, Sylvia BRUISTEN and David ŠMAJS. Complete genome sequences of two strains of Treponema pallidum subsp. pertenue from Ghana, Africa: Identical genome sequences in samples isolated more than 7 years apart. PLOS Neglected Tropical Diseases. San Francisco: Public Library of Science, 2017, vol. 11, No 9, p. 1-17. ISSN 1935-2735. Available from: https://dx.doi.org/10.1371/journal.pntd.0005894.

@article{1389299,
   author = {Strouhal, Michal and Paštěková, Lenka and Havlíčková, Pavla and Tenti, Paolo and Čejková, Darina and Rychlík, Ivan and Bruisten, Sylvia and Šmajs, David},
   article_location = {San Francisco},
   article_number = {9},
   doi = {http://dx.doi.org/10.1371/journal.pntd.0005894},
   keywords = {Treponema pallidum subsp pertenue},
   language = {eng},
   issn = {1935-2735},
   journal = {PLOS Neglected Tropical Diseases},
   title = {Complete genome sequences of two strains of Treponema pallidum subsp. pertenue from Ghana, Africa: Identical genome sequences in samples isolated more than 7 years apart},
   volume = {11},
   year = {2017}
}

TY  - JOUR
ID  - 1389299
AU  - Strouhal, Michal - Paštěková, Lenka - Havlíčková, Pavla - Tenti, Paolo - Čejková, Darina - Rychlík, Ivan - Bruisten, Sylvia - Šmajs, David
PY  - 2017
TI  - Complete genome sequences of two strains of Treponema pallidum subsp. pertenue from Ghana, Africa: Identical genome sequences in samples isolated more than 7 years apart
JF  - PLOS Neglected Tropical Diseases
VL  - 11
IS  - 9
SP  - 1-17
EP  - 1-17
PB  - Public Library of Science
SN  - 19352735
KW  - Treponema pallidum subsp pertenue
N2  - Treponema pallidum subsp. pertenue (TPE) is the causative agent of yaws, a multi-stage disease, endemic in tropical regions of Africa, Asia, Oceania, and South America. To date, four TPE strains have been completely sequenced including three TPE strains of human origin (Samoa D, CDC-2, and Gauthier) and one TPE strain (Fribourg-Blanc) isolated from a baboon. All TPE strains are highly similar to T. pallidum subsp. pallidum (TPA) strains. The mutation rate in syphilis and related treponemes has not been experimentally determined yet. Complete genomes of two TPE strains, CDC 2575 and Ghana-051, that infected patients in Ghana and were isolated in 1980 and 1988, respectively, were sequenced and analyzed. Both strains had identical consensus genome nucleotide sequences raising the question whether TPE CDC 2575 and Ghana-051 represent two different strains. Several lines of evidence support the fact that both strains represent independent samples including regions showing intrastrain heterogeneity (13 and 5 intrastrain heterogeneous sites in TPE Ghana-051 and TPE CDC 2575, respectively). Four of these heterogeneous sites were found in both genomes but the frequency of alternative alleles differed. The identical consensus genome sequences were used to estimate the upper limit of the yaws treponeme evolution rate, which was 4.1 x 10-10 nucleotide changes per site per generation. The estimated upper limit for the mutation rate of TPE was slightly lower than the mutation rate of E. coli, which was determined during a long-term experiment. Given the known diversity between TPA and TPE genomes and the assumption that both TPA and TPE have a similar mutation rate, the most recent common ancestor of syphilis and yaws treponemes appears to be more than ten thousand years old and likely even older.
ER  -

STROUHAL, Michal, Lenka PAŠTĚKOVÁ, Pavla HAVLÍČKOVÁ, Paolo TENTI, Darina ČEJKOVÁ, Ivan RYCHLÍK, Sylvia BRUISTEN and David ŠMAJS. Complete genome sequences of two strains of Treponema pallidum subsp. pertenue from Ghana, Africa: Identical genome sequences in samples isolated more than 7 years apart. \textit{PLOS Neglected Tropical Diseases}. San Francisco: Public Library of Science, 2017, vol.~11, No~9, p.~1-17. ISSN~1935-2735. Available from: https://dx.doi.org/10.1371/journal.pntd.0005894.

Detailed Information on Publication Record