J 2017

Deregulated expression of long non-coding RNA UCA1 in multiple myeloma

SEDLAŘÍKOVÁ, Lenka, Barbora GROMESOVÁ, Veronika KUBACZKOVÁ, Lenka RADOVÁ, Jana FILIPOVA et. al.

Basic information

Original name

Deregulated expression of long non-coding RNA UCA1 in multiple myeloma

Authors

SEDLAŘÍKOVÁ, Lenka (203 Czech Republic, belonging to the institution), Barbora GROMESOVÁ (203 Czech Republic, belonging to the institution), Veronika KUBACZKOVÁ (203 Czech Republic, belonging to the institution), Lenka RADOVÁ (203 Czech Republic, belonging to the institution), Jana FILIPOVA (203 Czech Republic), Jiří JARKOVSKÝ (203 Czech Republic, belonging to the institution), Lucie BROŽOVÁ (203 Czech Republic, belonging to the institution), Roberta VELICHOVÁ (703 Slovakia, belonging to the institution), Martina ALMÁŠI (203 Czech Republic), Miroslav PENKA (203 Czech Republic), Renata BEZDĚKOVÁ (203 Czech Republic), Martin ŠTORK (203 Czech Republic, belonging to the institution), Zdeněk ADAM (203 Czech Republic, belonging to the institution), Luděk POUR (203 Czech Republic, belonging to the institution), Marta KREJČÍ (203 Czech Republic, belonging to the institution), Petr KUGLÍK (203 Czech Republic, belonging to the institution), Roman HÁJEK (203 Czech Republic) and Sabina ŠEVČÍKOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

European Journal of Haematology, Hoboken, Wiley-Blackwell, 2017, 0902-4441

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30205 Hematology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.595

RIV identification code

RIV/00216224:14110/17:00095660

Organization unit

Faculty of Medicine

UT WoS

000408148200004

Keywords in English

biomarker; long non-coding RNA; multiple myeloma; plasma cells; prognosis; quantitative real-time PCR

Tags

Tags

International impact, Reviewed
Změněno: 20/3/2018 14:10, Soňa Böhmová

Abstract

V originále

ObjectivesLong non-coding RNAs (lncRNAs) are RNA transcripts longer than 200 nucleotides that are not translated into proteins. They are involved in pathogenesis of many diseases including cancer and have a potential to serve as diagnostic and prognostic markers. We aimed to investigate lncRNA expression profiles in bone marrow plasma cells (BMPCs) of newly diagnosed multiple myeloma (MM) patients in comparison to normal BMPCs of healthy donors (HD) in a three-phase biomarker study. MethodsExpression profile of 83 lncRNA was performed by RT2 lncRNA PCR Array (Qiagen), followed by quantitative real-time PCR using specific TaqMan non-coding RNA assays analyzing 84 newly diagnosed MM patients and 25 HD. ResultsOur analysis revealed dysregulation of two lncRNAs; NEAT1 (sensitivity of 55.0% and specificity of 79.0%) and UCA1 (sensitivity of 85.0% and specificity of 94.7%). UCA1 levels correlated with albumin and monoclonal immunoglobulin serum levels, cytogenetic aberrations, and survival of MM patients. ConclusionOur study suggests a possible prognostic impact of UCA1 expression levels on MM patients.

Links

NV15-29508A, research and development project
Name: Cirkulující nukleové kyseliny jako markery progrese mnohočetného myelomu