Inhibition of proteolysis by the hematophagous Eudiplozoon
nipponicum

a 2017

Inhibition of proteolysis by the hematophagous Eudiplozoon nipponicum

ILGOVÁ, Jana, Lucie JEDLIČKOVÁ, Hana DVOŘÁKOVÁ, Libor MIKEŠ, Michal BENOVICS et. al.

Basic information

Original name

Inhibition of proteolysis by the hematophagous Eudiplozoon nipponicum

Authors

ILGOVÁ, Jana (703 Slovakia, belonging to the institution), Lucie JEDLIČKOVÁ (203 Czech Republic), Hana DVOŘÁKOVÁ (203 Czech Republic), Libor MIKEŠ (203 Czech Republic), Michal BENOVICS (703 Slovakia, belonging to the institution), Pavel ROUDNICKÝ (203 Czech Republic, belonging to the institution), Jiří VOREL (203 Czech Republic, belonging to the institution), Libor VOJTEK (203 Czech Republic, belonging to the institution), Pavel HYRŠL (203 Czech Republic, belonging to the institution), Jiří SALÁT (203 Czech Republic, belonging to the institution), Milan GELNAR (203 Czech Republic, belonging to the institution) and Martin KAŠNÝ (203 Czech Republic, belonging to the institution)

Edition

8th International Symposium on Monogenea, 2017

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

ECIP - European Centre of IchthyoParasitology

RIV identification code

RIV/00216224:14310/17:00094966

Organization unit

Faculty of Science

ISBN

978-80-210-8666-1

Keywords in English

stefin; parasite; eudiplozoon; interaction host-parasite; inhibition;

Změněno: 24/9/2017 17:39, Mgr. Michal Benovics, Ph.D.

Abstract

V originále

Eudiplozoon nipponicum (Monogenea: Diplozoidae) is an obligatory hematophagous parasite of the common carp (Cyprinus carpio). The blood degradation by this species involves a cascade of cysteine and aspartic proteases hypothetically regulated by protease inhibitors (e.g. cystatins and stefins). These inhibitory molecules are also known to have impact on immunomodulation of the host and the repair of its tissue damaged by the parasite. Our study aims to reveal the biological function of the stefin of E. nipponicum which was detected in the transcriptome and excretory-secretory products of adult individuals. We prepared recombinant form of E. nipponicum stefin (rEnStef) in E. coli BL21 bacterial strain using pET19b expression plasmid vector. By adoption of fluorometric assay we observed efficient inhibition of cysteine peptidases (cathepsins L and B from E. nipponicum and mouse cathepsin L) via its conserved papain-binding domain. Surprisingly legumain (asparaginyl endopeptidase) inhibition was detected probably due to legumain-binding domain, untypical for stefins. rEnStef blocked proteolytic degradation of hemoglobin mediated by cysteine peptidases in the excretory-secretory products, soluble protein extracts from E. nipponicum and by recombinant cathepsins L3 and B of E. nipponicum, which manifests its role in blood digestion. rEnStef any effect on the activation of complement in carp’s plasma or oxidative burst in full blood studied using luminol-enhanced chemiluminescence. Significant downregulation of selected cytokines (IL-1beta, IL-8, TNF-alfa, IL-6 and IL-10) by LPS stimulated porcine alveolar macrophages and monocyte derived macrophages caused by rEnStef might indicate possible role of E. nipponicum stefin in immunomodulation of the host.

Links

GBP505/12/G112, research and development project

Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

Citovat

ILGOVÁ, Jana, Lucie JEDLIČKOVÁ, Hana DVOŘÁKOVÁ, Libor MIKEŠ, Michal BENOVICS, Pavel ROUDNICKÝ, Jiří VOREL, Libor VOJTEK, Pavel HYRŠL, Jiří SALÁT, Milan GELNAR and Martin KAŠNÝ. Inhibition of proteolysis by the hematophagous Eudiplozoon nipponicum. In 8th International Symposium on Monogenea. 2017. ISBN 978-80-210-8666-1.

@proceedings{1390168,
   author = {Ilgová, Jana and Jedličková, Lucie and Dvořáková, Hana and Mikeš, Libor and Benovics, Michal and Roudnický, Pavel and Vorel, Jiří and Vojtek, Libor and Hyršl, Pavel and Salát, Jiří and Gelnar, Milan and Kašný, Martin},
   booktitle = {8th International Symposium on Monogenea},
   keywords = {stefin; parasite; eudiplozoon; interaction host-parasite; inhibition;},
   language = {eng},
   isbn = {978-80-210-8666-1},
   title = {Inhibition of proteolysis by the hematophagous Eudiplozoon nipponicum},
   url = {http://ecip.cz/news/4},
   year = {2017}
}

TY  - CONF
ID  - 1390168
AU  - Ilgová, Jana - Jedličková, Lucie - Dvořáková, Hana - Mikeš, Libor - Benovics, Michal - Roudnický, Pavel - Vorel, Jiří - Vojtek, Libor - Hyršl, Pavel - Salát, Jiří - Gelnar, Milan - Kašný, Martin
PY  - 2017
TI  - Inhibition of proteolysis by the hematophagous Eudiplozoon nipponicum
SN  - 9788021086661
KW  - stefin
KW  - parasite
KW  - eudiplozoon
KW  - interaction host-parasite
KW  - inhibition;
UR  - http://ecip.cz/news/4
L2  - http://ecip.cz/news/4
N2  - Eudiplozoon nipponicum (Monogenea: Diplozoidae) is an obligatory hematophagous parasite of the common carp (Cyprinus carpio). The blood degradation by this species involves a cascade of cysteine and aspartic proteases hypothetically regulated by protease inhibitors (e.g. cystatins and stefins). These inhibitory molecules are also known to have impact on immunomodulation of the host and the repair of its tissue damaged by the parasite. Our study aims to reveal the biological function of the stefin of E. nipponicum which was detected in the transcriptome and excretory-secretory products of adult individuals. We prepared recombinant form of E. nipponicum stefin (rEnStef) in E. coli BL21 bacterial strain using pET19b expression plasmid vector. By adoption of fluorometric assay we observed efficient inhibition of cysteine peptidases (cathepsins L and B from E. nipponicum and mouse cathepsin L) via its conserved papain-binding domain. Surprisingly legumain (asparaginyl endopeptidase) inhibition was detected probably due to legumain-binding domain, untypical for stefins. rEnStef blocked proteolytic degradation of hemoglobin mediated by cysteine peptidases in the excretory-secretory products, soluble protein extracts from E. nipponicum and by recombinant cathepsins L3 and B of E. nipponicum, which manifests its role in blood digestion. rEnStef any effect on the activation of complement in carp’s plasma or oxidative burst in full blood studied using luminol-enhanced chemiluminescence. Significant downregulation of selected cytokines (IL-1beta, IL-8, TNF-alfa, IL-6 and IL-10) by LPS stimulated porcine alveolar macrophages and monocyte derived macrophages caused by rEnStef might indicate possible role of E. nipponicum stefin in immunomodulation of the host.
ER  -

ILGOVÁ, Jana, Lucie JEDLIČKOVÁ, Hana DVOŘÁKOVÁ, Libor MIKEŠ, Michal BENOVICS, Pavel ROUDNICKÝ, Jiří VOREL, Libor VOJTEK, Pavel HYRŠL, Jiří SALÁT, Milan GELNAR and Martin KAŠNÝ. Inhibition of proteolysis by the hematophagous Eudiplozoon nipponicum. In \textit{8th International Symposium on Monogenea}. 2017. ISBN~978-80-210-8666-1.

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