Detailed Information on Publication Record
2017
Changes of Cerebrospinal Fluid Peptides due to Tauopathy
MAJEROVA, Petra, Peter BARATH, Alena MICHALICOVA, Stanislav KATINA, Michal NOVAK et. al.Basic information
Original name
Changes of Cerebrospinal Fluid Peptides due to Tauopathy
Authors
MAJEROVA, Petra (703 Slovakia), Peter BARATH (703 Slovakia), Alena MICHALICOVA (703 Slovakia), Stanislav KATINA (703 Slovakia, guarantor, belonging to the institution), Michal NOVAK (703 Slovakia) and Andrej KOVAC (703 Slovakia)
Edition
Journal of Alzheimer’s Disease, Amsterdam, IOS Press, 2017, 1387-2877
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10103 Statistics and probability
Country of publisher
Netherlands
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 3.476
RIV identification code
RIV/00216224:14310/17:00095001
Organization unit
Faculty of Science
UT WoS
000402994400018
Keywords in English
Cerebrospinal fluid; LC-MALDI MS; peptidomics; rat model; tauopathy
Tags
International impact, Reviewed
Změněno: 5/4/2018 09:47, Ing. Nicole Zrilić
Abstract
V originále
Alzheimer’s disease (AD) and progressive supranuclear palsy are two common neurodegenerative tauopathies, and the most common cause of progressive brain dementia in elderly affecting more than 35 million people. The tauopathies are characterized by abnormal deposition of microtubule associated protein tau into intracellular neurofibrillary tangles composed mainly of the hyperphosphorylated form of the protein. The diagnosis of tauopathies is based on the presence of clinical features and pathological changes. Over the last decade, there has been an intensive search for novel biochemical markers for clinical diagnosis of AD and other tauopathies. In the present study, we used transgenic rat model for tauopathy expressing human truncated tau protein (aa 151–391/4R) to analyze the cerebrospinal fluid (CSF) peptidome using liquid chromatography – matrix assisted laser desorption/ionization mass spectrometry (LC-MALDI TOF/TOF). From 345 peptides, we identified a total of 175 proteins. Among them, 17 proteins were significantly altered in the CSF of transgenic rats. The following proteins were elevated in the CSF of transgenic rats when compared to the control animals: neurofilament light and medium chain, apolipoprotein E, gamma-synuclein, chromogranin A, reticulon-4, secretogranin-2, calsyntein-1 and -3, endothelin-3, neuroendocrine protein B72A, alpha-1-macroglobulin, and augurin. Interestingly most of the identified proteins were previously linked to AD and other tauopathies, indicating the significance of transgenic animals in biomarker validation.
Links
| GA15-06991S, research and development project |
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