ZAPLETAL, Ondřej, Zuzana TYLICHOVÁ, Jiří NEČA, Jiří KOHOUTEK, Miroslav MACHALA, A. MILCOVA, Michaela POKORNA, Jan TOPINKA, M.P. MOYER, Jiřina HOFMANOVÁ, Alois KOZUBÍK and Jan VONDRÁČEK. Butyrate alters expression of cytochrome P450 1A1 and metabolism of benzo[a] pyrene via its histone deacetylase activity in colon epithelial cell models. ARCHIVES OF TOXICOLOGY. HEIDELBERG: SPRINGER HEIDELBERG, vol. 91, No 5, p. 2135-2150. ISSN 0340-5761. doi:10.1007/s00204-016-1887-4. 2017.
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Basic information
Original name Butyrate alters expression of cytochrome P450 1A1 and metabolism of benzo[a] pyrene via its histone deacetylase activity in colon epithelial cell models
Authors ZAPLETAL, Ondřej (203 Czech Republic, belonging to the institution), Zuzana TYLICHOVÁ (203 Czech Republic, belonging to the institution), Jiří NEČA (203 Czech Republic), Jiří KOHOUTEK (203 Czech Republic), Miroslav MACHALA (203 Czech Republic), A. MILCOVA (203 Czech Republic), Michaela POKORNA (203 Czech Republic), Jan TOPINKA (203 Czech Republic), M.P. MOYER (840 United States of America), Jiřina HOFMANOVÁ (203 Czech Republic), Alois KOZUBÍK (203 Czech Republic) and Jan VONDRÁČEK (203 Czech Republic, guarantor).
Edition ARCHIVES OF TOXICOLOGY, HEIDELBERG, SPRINGER HEIDELBERG, 2017, 0340-5761.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10601 Cell biology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 5.728
RIV identification code RIV/00216224:14310/17:00097700
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1007/s00204-016-1887-4
UT WoS 000399875300008
Keywords in English CYP1A1; Butyrate; Polycyclic aromatic hydrocarbons; DNA adducts; Histone deacetylases; Colon epithelial cells
Tags NZ, rivok
Tags International impact, Reviewed
Changed by Changed by: Ing. Nicole Zrilić, učo 240776. Changed: 29/3/2018 15:21.
Abstract
Butyrate, a short-chain fatty acid produced by fermentation of dietary fiber, is an important regulator of colonic epithelium homeostasis. In this study, we investigated the impact of this histone deacetylase (HDAC) inhibitor on expression/activity of cytochrome P450 family 1 (CYP1) and on metabolism of carcinogenic polycyclic aromatic hydrocarbon, benzo[a] pyrene (BaP), in colon epithelial cells. Sodium butyrate (NaBt) strongly potentiated the BaP-induced expression of CYP1A1 in human colon carcinoma HCT116 cells. It also co-stimulated the 7-ethoxyresorufin-O-deethylase (EROD) activity induced by the 2,3,7,8-tetrachlorodibenzo-p-dioxin, a prototypical ligand of the aryl hydrocarbon receptor. Up-regulation of CYP1A1 expression/activity corresponded with an enhanced metabolism of BaP and formation of covalent DNA adducts. NaBt significantly potentiated CYP1A1 induction and/or metabolic activation of BaP also in other human colon cell models, colon adenoma AA/C1 cells, colon carcinoma HT-29 cells, or in NCM460D cell line derived from normal colon mucosa. Our results suggest that the effects of NaBt were due to its impact on histone acetylation, because additional HDAC inhibitors (trichostatin A and suberanilohydroxamic acid) likewise increased both the induction of EROD activity and formation of covalent DNA adducts. NaBt-induced acetylation of histone II3 (at Lys14) and histone II4 (at Lys16), two histone modifications modulated during activation of CYP1A1 transcription, and it reduced binding of HDAC1 to the enhancer region of CYP1A1 gene. This in vitro study suggests that butyrate, through modulation of histone acetylation, may potentiate induction of CYP1A1 expression, which might in turn alter the metabolism of BaP within colon epithelial cells.
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