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@article{1391002,
author = {Pavlová, Tereza and Vidová, Veronika and Bienertová Vašků, Julie and Janků, Petr and Almáši, Martina and Klánová, Jana and Spáčil, Zdeněk},
article_location = {Amsterdam},
article_number = {September},
doi = {http://dx.doi.org/10.1016/j.aca.2017.08.022},
keywords = {Tryptophan metabolism; Gut microbiome; Urinary metabolome; Methyl indole-3-acetate; Methyl indol-3-propionate; N-acetyltryptophan},
language = {eng},
issn = {0003-2670},
journal = {Analytica Chimica Acta},
title = {Urinary intermediates of tryptophan as indicators of the gut microbial metabolism},
url = {https://www.sciencedirect.com/science/article/pii/S0003267017309595?via%3Dihub},
volume = {987},
year = {2017}
}
TY - JOUR
ID - 1391002
AU - Pavlová, Tereza - Vidová, Veronika - Bienertová Vašků, Julie - Janků, Petr - Almáši, Martina - Klánová, Jana - Spáčil, Zdeněk
PY - 2017
TI - Urinary intermediates of tryptophan as indicators of the gut microbial metabolism
JF - Analytica Chimica Acta
VL - 987
IS - September
SP - 72-80
EP - 72-80
PB - Elsevier Science
SN - 00032670
KW - Tryptophan metabolism
KW - Gut microbiome
KW - Urinary metabolome
KW - Methyl indole-3-acetate
KW - Methyl indol-3-propionate
KW - N-acetyltryptophan
UR - https://www.sciencedirect.com/science/article/pii/S0003267017309595?via%3Dihub
L2 - https://www.sciencedirect.com/science/article/pii/S0003267017309595?via%3Dihub
N2 - While over 10% of the human metabolome is directly associated with the gut microbial metabolism, specific metabolites are largely uncharacterized. Therefore, methods for the identification and quantification of microbiota-associated metabolites in biological fluids such as urine or plasma are necessary in order to elucidate the molecular basis of host-microbiota interaction. In this study, we focused on the tryptophan metabolism, employing quantitative assays by ultra-high performance liquid chromatography (UHPLC) and tandem mass spectrometry, specifically selected reaction monitoring (SRM). Metabolite standards were utilized to generate SRM library for 16 intermediates of the tryptophan metabolism which were human endogenous as well as microbiota-associated based on the HMDB classification. Next, the SRM assays were utilized for screening in maternal urine samples and in dried urine specimens from neonates. The approach resulted in the discovery of microbiota-associated metabolites (methyl indole-3-acetate and methyl indol-3-propionate) previously unreported in urine samples and additionally in quantification of 8 intermediates of the tryptophan metabolism. To the best of our knowledge, this study represents the first attempt to explore previously unreported microbial metabolites in urine by UHPLC-SRM and novel methodology for simultaneous determination of microbiota-modulated component of Trp metabolism.
ER -
PAVLOVÁ, Tereza, Veronika VIDOVÁ, Julie BIENERTOVÁ VAŠKŮ, Petr JANKŮ, Martina ALMÁŠI, Jana KLÁNOVÁ and Zdeněk SPÁČIL. Urinary intermediates of tryptophan as indicators of the gut microbial metabolism. \textit{Analytica Chimica Acta}. Amsterdam: Elsevier Science, 2017, vol.~987, September, p.~72-80. ISSN~0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2017.08.022.
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