Urinary intermediates of tryptophan as indicators of the gut
microbial metabolism

J 2017

Urinary intermediates of tryptophan as indicators of the gut microbial metabolism

PAVLOVÁ, Tereza, Veronika VIDOVÁ, Julie BIENERTOVÁ VAŠKŮ, Petr JANKŮ, Martina ALMÁŠI et. al.

Basic information

Original name

Urinary intermediates of tryptophan as indicators of the gut microbial metabolism

Authors

PAVLOVÁ, Tereza (203 Czech Republic, belonging to the institution), Veronika VIDOVÁ (203 Czech Republic, belonging to the institution), Julie BIENERTOVÁ VAŠKŮ (203 Czech Republic, belonging to the institution), Petr JANKŮ (203 Czech Republic, belonging to the institution), Martina ALMÁŠI (203 Czech Republic, belonging to the institution), Jana KLÁNOVÁ (203 Czech Republic, belonging to the institution) and Zdeněk SPÁČIL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Analytica Chimica Acta, Amsterdam, Elsevier Science, 2017, 0003-2670

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.123

RIV identification code

RIV/00216224:14310/17:00095009

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.aca.2017.08.022

UT WoS

000410304700008

Keywords in English

Tryptophan metabolism; Gut microbiome; Urinary metabolome; Methyl indole-3-acetate; Methyl indol-3-propionate; N-acetyltryptophan

Abstract

V originále

While over 10% of the human metabolome is directly associated with the gut microbial metabolism, specific metabolites are largely uncharacterized. Therefore, methods for the identification and quantification of microbiota-associated metabolites in biological fluids such as urine or plasma are necessary in order to elucidate the molecular basis of host-microbiota interaction. In this study, we focused on the tryptophan metabolism, employing quantitative assays by ultra-high performance liquid chromatography (UHPLC) and tandem mass spectrometry, specifically selected reaction monitoring (SRM). Metabolite standards were utilized to generate SRM library for 16 intermediates of the tryptophan metabolism which were human endogenous as well as microbiota-associated based on the HMDB classification. Next, the SRM assays were utilized for screening in maternal urine samples and in dried urine specimens from neonates. The approach resulted in the discovery of microbiota-associated metabolites (methyl indole-3-acetate and methyl indol-3-propionate) previously unreported in urine samples and additionally in quantification of 8 intermediates of the tryptophan metabolism. To the best of our knowledge, this study represents the first attempt to explore previously unreported microbial metabolites in urine by UHPLC-SRM and novel methodology for simultaneous determination of microbiota-modulated component of Trp metabolism.

Links

CZ.02.1.01/0.0/0.0/16_013/0001761, interní kód MU

Name: RECETOX RI - OP VVV (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR, Priority axis 1: Strengthening capacities for high-quality research

EF15_003/0000469, research and development project

Name: Cetocoen Plus

GJ17-24592Y, research and development project

Name: Mapování interakcí mezi základními metabolickými pochody a střevní mikroflórou

Investor: Czech Science Foundation

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

PAVLOVÁ, Tereza, Veronika VIDOVÁ, Julie BIENERTOVÁ VAŠKŮ, Petr JANKŮ, Martina ALMÁŠI, Jana KLÁNOVÁ and Zdeněk SPÁČIL. Urinary intermediates of tryptophan as indicators of the gut microbial metabolism. Analytica Chimica Acta. Amsterdam: Elsevier Science, 2017, vol. 987, September, p. 72-80. ISSN 0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2017.08.022.

@article{1391002,
   author = {Pavlová, Tereza and Vidová, Veronika and Bienertová Vašků, Julie and Janků, Petr and Almáši, Martina and Klánová, Jana and Spáčil, Zdeněk},
   article_location = {Amsterdam},
   article_number = {September},
   doi = {http://dx.doi.org/10.1016/j.aca.2017.08.022},
   keywords = {Tryptophan metabolism; Gut microbiome; Urinary metabolome; Methyl indole-3-acetate; Methyl indol-3-propionate; N-acetyltryptophan},
   language = {eng},
   issn = {0003-2670},
   journal = {Analytica Chimica Acta},
   title = {Urinary intermediates of tryptophan as indicators of the gut microbial metabolism},
   url = {https://www.sciencedirect.com/science/article/pii/S0003267017309595?via%3Dihub},
   volume = {987},
   year = {2017}
}

TY  - JOUR
ID  - 1391002
AU  - Pavlová, Tereza - Vidová, Veronika - Bienertová Vašků, Julie - Janků, Petr - Almáši, Martina - Klánová, Jana - Spáčil, Zdeněk
PY  - 2017
TI  - Urinary intermediates of tryptophan as indicators of the gut microbial metabolism
JF  - Analytica Chimica Acta
VL  - 987
IS  - September
SP  - 72-80
EP  - 72-80
PB  - Elsevier Science
SN  - 00032670
KW  - Tryptophan metabolism
KW  - Gut microbiome
KW  - Urinary metabolome
KW  - Methyl indole-3-acetate
KW  - Methyl indol-3-propionate
KW  - N-acetyltryptophan
UR  - https://www.sciencedirect.com/science/article/pii/S0003267017309595?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0003267017309595?via%3Dihub
N2  - While over 10% of the human metabolome is directly associated with the gut microbial metabolism, specific metabolites are largely uncharacterized. Therefore, methods for the identification and quantification of microbiota-associated metabolites in biological fluids such as urine or plasma are necessary in order to elucidate the molecular basis of host-microbiota interaction. In this study, we focused on the tryptophan metabolism, employing quantitative assays by ultra-high performance liquid chromatography (UHPLC) and tandem mass spectrometry, specifically selected reaction monitoring (SRM). Metabolite standards were utilized to generate SRM library for 16 intermediates of the tryptophan metabolism which were human endogenous as well as microbiota-associated based on the HMDB classification. Next, the SRM assays were utilized for screening in maternal urine samples and in dried urine specimens from neonates. The approach resulted in the discovery of microbiota-associated metabolites (methyl indole-3-acetate and methyl indol-3-propionate) previously unreported in urine samples and additionally in quantification of 8 intermediates of the tryptophan metabolism. To the best of our knowledge, this study represents the first attempt to explore previously unreported microbial metabolites in urine by UHPLC-SRM and novel methodology for simultaneous determination of microbiota-modulated component of Trp metabolism.
ER  -

PAVLOVÁ, Tereza, Veronika VIDOVÁ, Julie BIENERTOVÁ VAŠKŮ, Petr JANKŮ, Martina ALMÁŠI, Jana KLÁNOVÁ and Zdeněk SPÁČIL. Urinary intermediates of tryptophan as indicators of the gut microbial metabolism. \textit{Analytica Chimica Acta}. Amsterdam: Elsevier Science, 2017, vol.~987, September, p.~72-80. ISSN~0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2017.08.022.

Detailed Information on Publication Record