PAVLOVÁ, Tereza, Veronika VIDOVÁ, Julie BIENERTOVÁ VAŠKŮ, Petr JANKŮ, Martina ALMÁŠI, Jana KLÁNOVÁ and Zdeněk SPÁČIL. Urinary intermediates of tryptophan as indicators of the gut microbial metabolism. Analytica Chimica Acta. Amsterdam: Elsevier Science, vol. 987, September, p. 72-80. ISSN 0003-2670. doi:10.1016/j.aca.2017.08.022. 2017.
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Basic information
Original name Urinary intermediates of tryptophan as indicators of the gut microbial metabolism
Authors PAVLOVÁ, Tereza (203 Czech Republic, belonging to the institution), Veronika VIDOVÁ (203 Czech Republic, belonging to the institution), Julie BIENERTOVÁ VAŠKŮ (203 Czech Republic, belonging to the institution), Petr JANKŮ (203 Czech Republic, belonging to the institution), Martina ALMÁŠI (203 Czech Republic, belonging to the institution), Jana KLÁNOVÁ (203 Czech Republic, belonging to the institution) and Zdeněk SPÁČIL (203 Czech Republic, guarantor, belonging to the institution).
Edition Analytica Chimica Acta, Amsterdam, Elsevier Science, 2017, 0003-2670.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10406 Analytical chemistry
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 5.123
RIV identification code RIV/00216224:14310/17:00095009
Organization unit Faculty of Science
UT WoS 000410304700008
Keywords in English Tryptophan metabolism; Gut microbiome; Urinary metabolome; Methyl indole-3-acetate; Methyl indol-3-propionate; N-acetyltryptophan
Tags NZ, rivok
Tags International impact, Reviewed
Changed by Changed by: Ing. Nicole Zrilić, učo 240776. Changed: 12/4/2018 17:51.
While over 10% of the human metabolome is directly associated with the gut microbial metabolism, specific metabolites are largely uncharacterized. Therefore, methods for the identification and quantification of microbiota-associated metabolites in biological fluids such as urine or plasma are necessary in order to elucidate the molecular basis of host-microbiota interaction. In this study, we focused on the tryptophan metabolism, employing quantitative assays by ultra-high performance liquid chromatography (UHPLC) and tandem mass spectrometry, specifically selected reaction monitoring (SRM). Metabolite standards were utilized to generate SRM library for 16 intermediates of the tryptophan metabolism which were human endogenous as well as microbiota-associated based on the HMDB classification. Next, the SRM assays were utilized for screening in maternal urine samples and in dried urine specimens from neonates. The approach resulted in the discovery of microbiota-associated metabolites (methyl indole-3-acetate and methyl indol-3-propionate) previously unreported in urine samples and additionally in quantification of 8 intermediates of the tryptophan metabolism. To the best of our knowledge, this study represents the first attempt to explore previously unreported microbial metabolites in urine by UHPLC-SRM and novel methodology for simultaneous determination of microbiota-modulated component of Trp metabolism.
CZ.02.1.01/0.0/0.0/16_013/0001761, interní kód MUName: RECETOX RI - OP VVV (Acronym: RECETOX RI)
Investor: Ministry of Education, Youth and Sports of the CR, Priority axis 1: Strengthening capacities for high-quality research
EF15_003/0000469, research and development projectName: Cetocoen Plus
GJ17-24592Y, research and development projectName: Mapování interakcí mezi základními metabolickými pochody a střevní mikroflórou
Investor: Czech Science Foundation
LM2015051, research and development projectName: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)
Investor: Ministry of Education, Youth and Sports of the CR
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