Detailed Information on Publication Record
2017
A review on mass spectrometry-based quantitative proteomics: Targeted and data independent acquisition
VIDOVÁ, Veronika and Zdeněk SPÁČILBasic information
Original name
A review on mass spectrometry-based quantitative proteomics: Targeted and data independent acquisition
Authors
VIDOVÁ, Veronika (203 Czech Republic, belonging to the institution) and Zdeněk SPÁČIL (203 Czech Republic, guarantor, belonging to the institution)
Edition
Analytica Chimica Acta, Amsterdam, Elsevier Science publishers, 2017, 0003-2670
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10406 Analytical chemistry
Country of publisher
Netherlands
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 5.123
RIV identification code
RIV/00216224:14310/17:00097721
Organization unit
Faculty of Science
UT WoS
000398434700002
Keywords in English
Targeted quantitative proteomics tutorial; Selected/multiple reaction monitoring (SRM/MRM); Parallel reaction monitoring (PRM); Tandem mass spectrometry (MS/MS); Data independent acquisition (DIA)
Tags
International impact, Reviewed
Změněno: 27/3/2018 21:42, Ing. Nicole Zrilić
Abstract
V originále
Mass spectrometry (MS) based proteomics have achieved a near- complete proteome coverage in humans and in several other organisms, producing a wealth of information stored in databases and bioinformatics resources. Recent implementation of selected/multiple reaction monitoring (SRM/MRM) technology in targeted proteomics introduced the possibility of quantitatively follow-up specific protein targets in a hypothesis-driven experiment. In contrast to immunoaffinity-based workflows typically used in biological and clinical research for protein quantification, SRM/MRM is characterized by high selectivity, large capacity for multiplexing (approx.200 proteins per analysis) and rapid, cost-effective transition from assay development to deployment. The concept of SRM/MRM utilizes triple quadrupole (QqQ) mass analyzer to provide inherent reproducibility, unparalleled sensitivity and selectivity to efficiently differentiate isoforms, post-translational modifications and mutated forms of proteins. SRMlike targeted acquisitions such as parallel reaction monitoring (PRM) are pioneered on high resolution/accurate mass (HR/AM) platforms based on the quadrupole-orbitrap (Q-orbitrap) mass spectrometer. The expansion of HR/AM also caused development in data independent acquisition (DIA). This review presents a step-by-step tutorial on development of SRM/MRM protein assay intended for researchers without prior experience in proteomics. We discus practical aspects of SRM-based quantitative proteomics workflow, summarize milestones in basic biological and medical research as well as recent trends and emerging techniques.
Links
| EF15_003/0000469, research and development project |
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| LM2015051, research and development project |
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