| DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Lukáš KUČERA, Jitka OSIČKOVÁ, Jana CHOVANCOVÁ, Tomáš LOJA, Jiří MAYER, Michael DOUBEK and Marek JOUKAL. Local chemical sympathectomy of rat bone marrow and its effect on marrow cell composition (Local chemical sympathectomy of the rat bone marrow and its effect to marrow cell composition). AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL. AMSTERDAM: ELSEVIER SCIENCE BV, 2017, vol. 206, SEP 2017, p. 19-27. ISSN 1566-0702. Available from: https://dx.doi.org/10.1016/j.autneu.2017.06.003. |
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@article{1391016,
author = {Dubový, Petr and Klusáková, Ilona and Kučera, Lukáš and Osičková, Jitka and Chovancová, Jana and Loja, Tomáš and Mayer, Jiří and Doubek, Michael and Joukal, Marek},
article_location = {AMSTERDAM},
article_number = {SEP 2017},
doi = {http://dx.doi.org/10.1016/j.autneu.2017.06.003},
keywords = {Guanethidine; Osmotic mini pump; Sympathetic innervation; Endothelial cells},
language = {eng},
issn = {1566-0702},
journal = {AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL},
title = {Local chemical sympathectomy of rat bone marrow and its effect on marrow cell composition},
url = {https://doi.org/10.1016/j.autneu.2017.06.003},
volume = {206},
year = {2017}
}
TY - JOUR
ID - 1391016
AU - Dubový, Petr - Klusáková, Ilona - Kučera, Lukáš - Osičková, Jitka - Chovancová, Jana - Loja, Tomáš - Mayer, Jiří - Doubek, Michael - Joukal, Marek
PY - 2017
TI - Local chemical sympathectomy of rat bone marrow and its effect on marrow cell composition
JF - AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL
VL - 206
IS - SEP 2017
SP - 19-27
EP - 19-27
PB - ELSEVIER SCIENCE BV
SN - 15660702
KW - Guanethidine
KW - Osmotic mini pump
KW - Sympathetic innervation
KW - Endothelial cells
UR - https://doi.org/10.1016/j.autneu.2017.06.003
L2 - https://doi.org/10.1016/j.autneu.2017.06.003
N2 - Existing experimental studies of the effect of sympathetic nerve fibers on bone marrow cells are based on the systemic administration of neurotoxic 6-hydroxydopamine. The method of global chemical sympathectomy has some serious disadvantages and could lead to questionable results. We describe a new method of local chemical sympathectomy of rat femoral bone marrow using guanethidine (Ismelin) delivery using an osmotic mini pump. Local guanethidine treatment for 14 days led to complete elimination of sympathetic fibers in femoral bone marrow in contrast to bone marrow of contralateral or naive femurs. Ablation of sympathetic fibers was associated with a loss of rat endothelial cell marker (RECA) indicating immunophenotype changes in blood vessel endothelial cells, but no significant effect of guanethidine was found on the survival of endothelial cells and mesenchymal stem cells in vitro. Moreover, local guanethidine treatment also elicited a significant reduction of Nestin +/SDF1 + mesenchymal stem cells and c-Kit +/CD90 + hematopoietic stem cells in femoral bone marrow. Tissue-specific chemical sympathectomy of rat bone marrow by guanethidine overcomes some of the drawbacks of systemic administration of neurotoxic compounds like 6-hydroxydopamine and delivers unequivocal evidence on the effects of sympathetic innervation on the cell content of bone marrow.
ER -
DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Lukáš KUČERA, Jitka OSIČKOVÁ, Jana CHOVANCOVÁ, Tomáš LOJA, Jiří MAYER, Michael DOUBEK and Marek JOUKAL. Local chemical sympathectomy of rat bone marrow and its effect on marrow cell composition (Local chemical sympathectomy of the rat bone marrow and its effect to marrow cell composition). \textit{AUTONOMIC NEUROSCIENCE-BASIC \&{}amp; CLINICAL}. AMSTERDAM: ELSEVIER SCIENCE BV, 2017, vol.~206, SEP 2017, p.~19-27. ISSN~1566-0702. Available from: https://dx.doi.org/10.1016/j.autneu.2017.06.003.
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