Local chemical sympathectomy of rat bone marrow and its effect
on marrow cell composition

J 2017

Local chemical sympathectomy of rat bone marrow and its effect on marrow cell composition

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Lukáš KUČERA, Jitka OSIČKOVÁ, Jana CHOVANCOVÁ et. al.

Basic information

Original name

Local chemical sympathectomy of rat bone marrow and its effect on marrow cell composition

Authors

DUBOVÝ, Petr (203 Czech Republic, guarantor, belonging to the institution), Ilona KLUSÁKOVÁ (203 Czech Republic, belonging to the institution), Lukáš KUČERA (203 Czech Republic, belonging to the institution), Jitka OSIČKOVÁ (203 Czech Republic), Jana CHOVANCOVÁ (203 Czech Republic), Tomáš LOJA (703 Slovakia), Jiří MAYER (203 Czech Republic), Michael DOUBEK (203 Czech Republic) and Marek JOUKAL (203 Czech Republic, belonging to the institution)

Edition

AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL, AMSTERDAM, ELSEVIER SCIENCE BV, 2017, 1566-0702

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30103 Neurosciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.605

RIV identification code

RIV/00216224:14110/17:00097722

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.autneu.2017.06.003

UT WoS

000408178900003

Keywords in English

Guanethidine; Osmotic mini pump; Sympathetic innervation; Endothelial cells

Abstract

V originále

Existing experimental studies of the effect of sympathetic nerve fibers on bone marrow cells are based on the systemic administration of neurotoxic 6-hydroxydopamine. The method of global chemical sympathectomy has some serious disadvantages and could lead to questionable results. We describe a new method of local chemical sympathectomy of rat femoral bone marrow using guanethidine (Ismelin) delivery using an osmotic mini pump. Local guanethidine treatment for 14 days led to complete elimination of sympathetic fibers in femoral bone marrow in contrast to bone marrow of contralateral or naive femurs. Ablation of sympathetic fibers was associated with a loss of rat endothelial cell marker (RECA) indicating immunophenotype changes in blood vessel endothelial cells, but no significant effect of guanethidine was found on the survival of endothelial cells and mesenchymal stem cells in vitro. Moreover, local guanethidine treatment also elicited a significant reduction of Nestin +/SDF1 + mesenchymal stem cells and c-Kit +/CD90 + hematopoietic stem cells in femoral bone marrow. Tissue-specific chemical sympathectomy of rat bone marrow by guanethidine overcomes some of the drawbacks of systemic administration of neurotoxic compounds like 6-hydroxydopamine and delivers unequivocal evidence on the effects of sympathetic innervation on the cell content of bone marrow.

Links

LD15144, research and development project

Name: Buněčné a nebuněčné základy pro regeneraci kostí a zubů (Acronym: TissueENG)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

ROZV/24/LF3/2014, interní kód MU

Name: Je inervace kostní dřeně významný faktor ovlivňující hibernaci a uvolnění hematopoetických kmenových buněk?

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Lukáš KUČERA, Jitka OSIČKOVÁ, Jana CHOVANCOVÁ, Tomáš LOJA, Jiří MAYER, Michael DOUBEK and Marek JOUKAL. Local chemical sympathectomy of rat bone marrow and its effect on marrow cell composition (Local chemical sympathectomy of the rat bone marrow and its effect to marrow cell composition). AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL. AMSTERDAM: ELSEVIER SCIENCE BV, 2017, vol. 206, SEP 2017, p. 19-27. ISSN 1566-0702. Available from: https://dx.doi.org/10.1016/j.autneu.2017.06.003.

@article{1391016,
   author = {Dubový, Petr and Klusáková, Ilona and Kučera, Lukáš and Osičková, Jitka and Chovancová, Jana and Loja, Tomáš and Mayer, Jiří and Doubek, Michael and Joukal, Marek},
   article_location = {AMSTERDAM},
   article_number = {SEP 2017},
   doi = {http://dx.doi.org/10.1016/j.autneu.2017.06.003},
   keywords = {Guanethidine; Osmotic mini pump; Sympathetic innervation; Endothelial cells},
   language = {eng},
   issn = {1566-0702},
   journal = {AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL},
   title = {Local chemical sympathectomy of rat bone marrow and its effect on marrow cell composition},
   url = {https://doi.org/10.1016/j.autneu.2017.06.003},
   volume = {206},
   year = {2017}
}

TY  - JOUR
ID  - 1391016
AU  - Dubový, Petr - Klusáková, Ilona - Kučera, Lukáš - Osičková, Jitka - Chovancová, Jana - Loja, Tomáš - Mayer, Jiří - Doubek, Michael - Joukal, Marek
PY  - 2017
TI  - Local chemical sympathectomy of rat bone marrow and its effect on marrow cell composition
JF  - AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL
VL  - 206
IS  - SEP 2017
SP  - 19-27
EP  - 19-27
PB  - ELSEVIER SCIENCE BV
SN  - 15660702
KW  - Guanethidine
KW  - Osmotic mini pump
KW  - Sympathetic innervation
KW  - Endothelial cells
UR  - https://doi.org/10.1016/j.autneu.2017.06.003
L2  - https://doi.org/10.1016/j.autneu.2017.06.003
N2  - Existing experimental studies of the effect of sympathetic nerve fibers on bone marrow cells are based on the systemic administration of neurotoxic 6-hydroxydopamine. The method of global chemical sympathectomy has some serious disadvantages and could lead to questionable results. We describe a new method of local chemical sympathectomy of rat femoral bone marrow using guanethidine (Ismelin) delivery using an osmotic mini pump. Local guanethidine treatment for 14 days led to complete elimination of sympathetic fibers in femoral bone marrow in contrast to bone marrow of contralateral or naive femurs. Ablation of sympathetic fibers was associated with a loss of rat endothelial cell marker (RECA) indicating immunophenotype changes in blood vessel endothelial cells, but no significant effect of guanethidine was found on the survival of endothelial cells and mesenchymal stem cells in vitro. Moreover, local guanethidine treatment also elicited a significant reduction of Nestin +/SDF1 + mesenchymal stem cells and c-Kit +/CD90 + hematopoietic stem cells in femoral bone marrow. Tissue-specific chemical sympathectomy of rat bone marrow by guanethidine overcomes some of the drawbacks of systemic administration of neurotoxic compounds like 6-hydroxydopamine and delivers unequivocal evidence on the effects of sympathetic innervation on the cell content of bone marrow.
ER  -

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Lukáš KUČERA, Jitka OSIČKOVÁ, Jana CHOVANCOVÁ, Tomáš LOJA, Jiří MAYER, Michael DOUBEK and Marek JOUKAL. Local chemical sympathectomy of rat bone marrow and its effect on marrow cell composition (Local chemical sympathectomy of the rat bone marrow and its effect to marrow cell composition). \textit{AUTONOMIC NEUROSCIENCE-BASIC \&{}amp; CLINICAL}. AMSTERDAM: ELSEVIER SCIENCE BV, 2017, vol.~206, SEP 2017, p.~19-27. ISSN~1566-0702. Available from: https://dx.doi.org/10.1016/j.autneu.2017.06.003.

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