Polymer Pencil Graphite Electrode in the Study of
Electrochemical Processes of Guanine Analogues

D 2017

Polymer Pencil Graphite Electrode in the Study of Electrochemical Processes of Guanine Analogues

TRNKOVÁ, Libuše, Mehdi RAVANDEH and Jan HRBÁČ

Basic information

Original name

Polymer Pencil Graphite Electrode in the Study of Electrochemical Processes of Guanine Analogues

Authors

TRNKOVÁ, Libuše, Mehdi RAVANDEH and Jan HRBÁČ

Edition

1. vyd. Providence, 68th Annual Meeting of the International Society of Electrochemistry, 1 pp. 2017

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10403 Physical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

electronic version available online

Organization unit

Faculty of Science

Keywords (in Czech)

guanin; polymerní pentelková elektroda; cyklická voltametrie; elektronová mikroskopie; Ramanova spektroskopie

Keywords in English

guanine; polymer pencil graphite electrode; cyclic volatmmetry; electron microscopy; Raman spectroscopy

Abstract

V originále

Understanding the oxidation processes of purines and their derivatives is primary for the improvement of electrochemical methods of genetic and food materials analysis. However, it is generally known that guanine is one of the four main nucleobases found in the nucleic acids DNA and RNA, but it is less known that G, G-based purines, including the nucleoside and the nucleotide monophosphate play an important role in diet-related diuseases, helath of cardiovascular nad digestive systems and in neurodegenerative diseases. Electrochemical techniques in connection with carbon electrodes are powerful methods for analysis of G and its derivatives. Our previous research revelaed that a polymer pencil graphite electrode can serve as a sensitive and disposable sensor for purines, including G and its derivatives. In this work, the electrochemical oxidation processes of G, guanosine and guanosine monophosphate and their deoxy-derivatives were investigated. The dependence of oxidation signals on experimental conditions such as scan rate, electrode surface pretreatment, addition of Cu(II) ions, adsorption, pH and the presence of structural increments (ribofuranosyl-, deoxyribofuranosyl-, and phosphate group) alolowed us to evaluate:(i) kinetics and mechanism of G and GMP oxidation (electron and proton numbers, coefficients of electro-transfer, the role of electrode surface), (ii) the effect of adsorption and (iii) the effect of oxo, deoxy, and phosphate groups on oxidation responses. We can conclude that polymer pencil lead is a prospective electrode material with good stability and reproducibility not only for analysis of G and its analogues but also for whole purine group.

Links

LD15058, research and development project

Name: Příprava substrátů pro povrchově zesílenou Ramanovu spektroskopii pomocí elektrochemických, elektroforetických a jiskrový výboj využívajících technik. (Acronym: ELE-SERS)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/1237/2016, interní kód MU

Name: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2017

Investor: Masaryk University, Category A

Citovat

TRNKOVÁ, Libuše, Mehdi RAVANDEH and Jan HRBÁČ. Polymer Pencil Graphite Electrode in the Study of Electrochemical Processes of Guanine Analogues. Online. In 68th Annual Meeting of the International Society of Electrochemistry. 1st ed. Providence, 2017, 1 pp.

@inproceedings{1391527,
   author = {Trnková, Libuše and Ravandeh, Mehdi and Hrbáč, Jan},
   address = {Providence},
   booktitle = {68th Annual Meeting of the International Society of Electrochemistry},
   edition = {1},
   keywords = {guanine; polymer pencil graphite electrode; cyclic volatmmetry; electron microscopy; Raman spectroscopy},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {Providence},
   note = {Nepřenášet do RIV - není na WoS ani Scopus, neodpovídá definici druhů výsledků dle M17+},
   title = {Polymer Pencil Graphite Electrode in the Study of Electrochemical Processes of Guanine Analogues},
   year = {2017}
}

TY  - JOUR
ID  - 1391527
AU  - Trnková, Libuše - Ravandeh, Mehdi - Hrbáč, Jan
PY  - 2017
TI  - Polymer Pencil Graphite Electrode in the Study of Electrochemical Processes of Guanine Analogues
CY  - Providence
N1  - Nepřenášet do RIV - není na WoS ani Scopus, neodpovídá definici druhů výsledků dle M17+
KW  - guanine
KW  - polymer pencil graphite electrode
KW  - cyclic volatmmetry
KW  - electron microscopy
KW  - Raman spectroscopy
N2  - Understanding the oxidation processes of purines and their derivatives is primary for the improvement of electrochemical methods of genetic and food materials analysis. However, it is generally known that guanine is one of the four main nucleobases found in the nucleic acids DNA and RNA, but it is less known that G, G-based purines, including the nucleoside and the nucleotide monophosphate play an important role in diet-related diuseases, helath of cardiovascular nad digestive systems and in neurodegenerative diseases. Electrochemical techniques in connection with carbon electrodes are powerful methods for analysis of G and its derivatives. Our previous research revelaed that a polymer pencil graphite electrode can serve as a sensitive and disposable sensor for purines, including G and its derivatives. In this work, the electrochemical oxidation processes of G, guanosine and guanosine monophosphate and their deoxy-derivatives were investigated. The dependence of oxidation signals on experimental conditions such as scan rate, electrode surface pretreatment, addition of Cu(II) ions, adsorption, pH and the presence of structural increments (ribofuranosyl-, deoxyribofuranosyl-, and phosphate group) alolowed us to evaluate:(i) kinetics and mechanism of G and GMP oxidation (electron and proton numbers, coefficients of electro-transfer, the role of electrode surface), (ii) the effect of adsorption and (iii) the effect of oxo, deoxy, and phosphate groups on oxidation responses. We can conclude that polymer pencil lead is a prospective electrode material with good stability and reproducibility not only for analysis of G and its analogues but also for whole purine group.
ER  -

TRNKOVÁ, Libuše, Mehdi RAVANDEH and Jan HRBÁČ. Polymer Pencil Graphite Electrode in the Study of Electrochemical Processes of Guanine Analogues. Online. In \textit{68th Annual Meeting of the International Society of Electrochemistry}. 1st ed. Providence, 2017, 1 pp.

Detailed Information on Publication Record