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@inproceedings{1391563,
author = {Botka, Tibor and Růžičková, Vladislava and Svobodová, Karla and Petráš, Petr and Čejková, Darina},
address = {Brno},
booktitle = {The Biomania Student Scientific Meeting 2017},
editor = {Jan Škoda, Markéta Wayhelová, Dana Štveráková, Tomáš Buryška, Martin Benešík, Pavel Dvořák, Pavel Reichman},
keywords = {ETB plasmid; exfoliative toxin B; phylogeny; genome analysis},
howpublished = {tištěná verze "print"},
language = {eng},
location = {Brno},
isbn = {978-80-210-8737-8},
note = {Nepřenášíme do RIV, výsledek má jen 1 stranu, podle metodiky M17+ musí mít stať ve sborníku min. 2 strany.},
pages = {74-74},
publisher = {Masaryk University},
title = {Analysis of new types of exfoliative toxin B-encoding plasmids carried by impetigo strains of Staphylococcus aureus},
year = {2017}
}
TY - JOUR
ID - 1391563
AU - Botka, Tibor - Růžičková, Vladislava - Svobodová, Karla - Petráš, Petr - Čejková, Darina
PY - 2017
TI - Analysis of new types of exfoliative toxin B-encoding plasmids carried by impetigo strains of Staphylococcus aureus
PB - Masaryk University
CY - Brno
SN - 9788021087378
N1 - Nepřenášíme do RIV, výsledek má jen 1 stranu, podle metodiky M17+ musí mít stať ve sborníku min. 2 strany.
KW - ETB plasmid
KW - exfoliative toxin B
KW - phylogeny
KW - genome analysis
N2 - Bullous impetigo and staphylococcal scalded skin syndrome are diseases caused by Staphylococcus aureus strains producing exfoliative toxins A and/or B. These toxins are encoded by prophages and plasmids, respectively. We analyzed a set of exfoliative toxin B-encoding plasmids (ETB plasmids) isolated from the impetigo strains implicated in outbreaks of epidermolytic diseases in several Czech maternity hospitals. Restriction endonuclease analysis, DNA sequencing and PCR typing, as well as bioinformatics tools were used for characterization and comparison of the plasmids. Most of them, similar to the archetypal plasmid pETBTY4, formed the main lineage. Sharing a same core sequence, they differed from each other by extra DNA content. The plasmids of this lineage were carried by strains of clonal complex CC121, often along with the prophages encoding exfoliative toxin A. From a prophageless strain, a novel plasmid carrying gene cluster for conjugation and new variants of virulence genes was isolated. It was characterized in detail and could be regarded as a source of a new lineage of ETB plasmids. Based on our findings, we have designed a helpful assay, which enables the precise detection of the novel gene variant for exfoliative toxin B, and facilitates the identification of all described ETB plasmid types. Identified sequence variations enable us to reveal the diversity of ETB plasmids, which are the substantial source of virulence factors in the population of impetigo S. aureus strains.
ER -
BOTKA, Tibor, Vladislava RŮŽIČKOVÁ, Karla SVOBODOVÁ, Petr PETRÁŠ and Darina ČEJKOVÁ. Analysis of new types of exfoliative toxin B-encoding plasmids carried by impetigo strains of Staphylococcus aureus. In Jan Škoda, Markéta Wayhelová, Dana Štveráková, Tomáš Buryška, Martin Benešík, Pavel Dvořák, Pavel Reichman. \textit{The Biomania Student Scientific Meeting 2017}. Brno: Masaryk University, 2017, p.~74. ISBN~978-80-210-8737-8.
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