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@proceedings{1391575, author = {Hyršl, Pavel and Ibrahim, Emad and Dobeš, Pavel and Kunc, Martin and Kodrík, Dalibor}, booktitle = {25th European Drosophila Research Conference}, keywords = {Drosophila; nematodes; immunity; insect; adipokinetic hormone}, language = {eng}, title = {Adipokinetic hormone and adenosine in Drosophila melanogaster as a part of defence reaction against nematobacterial infection}, year = {2017} }
TY - CONF ID - 1391575 AU - Hyršl, Pavel - Ibrahim, Emad - Dobeš, Pavel - Kunc, Martin - Kodrík, Dalibor PY - 2017 TI - Adipokinetic hormone and adenosine in Drosophila melanogaster as a part of defence reaction against nematobacterial infection KW - Drosophila KW - nematodes KW - immunity KW - insect KW - adipokinetic hormone N2 - Entomopathogenic nematodes and their associated bacteria comprise together a highly pathogenic complex which is able to invade and kill insect host within two days. Both bacteria and nematodes produce a variety of factors interacting with the insect immune system. It is known that they activate anti-stress defence where adipokinetic hormone (AKH), important stress hormone responsible for keeping homeostasis in insect body, and adenosine, a purine nucleotide that serves as signalling factor in anti-stress reaction, play a role. Drosophila melanogaster mutants with non-functional AKH (AKH-def) and impaired adenosine receptor (AdoR-def), and two nematodal species Steinernema carpocapsae and Heterorhabditis bacteriophora were used in our study. The results revealed that both mutants showed significantly lower mortality after infection than the control, and reversely, application of external AKH significantly increased the mortality. We have found that the AKH absence reduced production of nutrients into the haemolymph, which inhibited the development of infection, and vice versa the application of AKH restored production of energy rich metabolites and supported the infection. Indeed, our experiments indicated clear stimulatory role of AKH, and minor modulatory role of adenosine in regulation of the trehalose level in Drosophila haemolymph. The nematode infection was accompanied by higher production of AKH, by increasing of anti-oxidative activity and by raising of nutrients in haemolymph which affected also larval locomotion as we detected by FTIR-based tracking method. Supported by the grant 17-03253S (Czech Science Foundation) and by the Missions Department in Cairo, Egypt. ER -
HYRŠL, Pavel, Emad IBRAHIM, Pavel DOBEŠ, Martin KUNC and Dalibor KODRÍK. Adipokinetic hormone and adenosine in Drosophila melanogaster as a part of defence reaction against nematobacterial infection. In \textit{25th European Drosophila Research Conference}. 2017.
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