gamma H2AX/53BP1 foci as a potential pre-treatment marker of
HNSCC tumors radiosensitivity - preliminary methodological
study and discussion

J 2017

gamma H2AX/53BP1 foci as a potential pre-treatment marker of HNSCC tumors radiosensitivity - preliminary methodological study and discussion

FALK, M., Zuzana HORÁKOVÁ, Markéta SVOBODOVÁ, Michal MASAŘÍK, O. KOPECNA et. al.

Basic information

Original name

gamma H2AX/53BP1 foci as a potential pre-treatment marker of HNSCC tumors radiosensitivity - preliminary methodological study and discussion

Authors

FALK, M. (203 Czech Republic), Zuzana HORÁKOVÁ (203 Czech Republic, belonging to the institution), Markéta SVOBODOVÁ (203 Czech Republic, belonging to the institution), Michal MASAŘÍK (203 Czech Republic, guarantor, belonging to the institution), O. KOPECNA (203 Czech Republic), Jaromír GUMULEC (203 Czech Republic, belonging to the institution), Martina RAUDENSKÁ (203 Czech Republic, belonging to the institution), D. DEPES (203 Czech Republic), A. BACIKOVA (203 Czech Republic), I. FALKOVA (203 Czech Republic) and Hana BINKOVÁ (203 Czech Republic, belonging to the institution)

Edition

The European Physical Journal D, NEW YORK, Springer Berlin Heidelberg, 2017, 1434-6060

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10306 Optics

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.393

RIV identification code

RIV/00216224:14110/17:00095036

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1140/epjd/e2017-80073-2

UT WoS

000411083200015

Keywords in English

HNSCC tumor

Abstract

V originále

In order to improve patients' post-treatment quality of life, a shift from surgery to non-surgical (chemo) radio-treatment is recognized in head and neck oncology. However, about half of HNSCC tumors are resistant to irradiation and an efficient marker of individual tumor radiosensitivity is still missing. We analyzed whether various parameters of DNA double strand break (DSB) repair determined in vitro can predict, prior to clinical treatment initiation, the radiosensitivity of tumors. We compared formation and decrease of gamma H2AX/53BP1 foci in 48 h after irradiating tumor cell primocultures with 2 Gy of gamma-rays. To better understand complex tumor behavior, three different cell type primocultures - CD90(-), CD90(+), and a mixed culture of these cells - were isolated from 1 clinically radioresistant, 2 radiosensitive, and 4 undetermined HPV-HNSCC tumors and followed separately. While DSB repair was delayed and the number of persisting DSBs increased in the radiosensitive tumors, the results for the radioresistant tumor were similar to cultured normal human skin fibroblasts. Hence, DSB repair kinetics/efficiency may correlate with clinical response to radiotherapy for a subset of HNSCC tumors but the size (and therefore practical relevance) of this subset remains to be determined. The same is true for contribution of different cell type primocultures to tumor radioresistance.

Links

GA16-12454S, research and development project

Name: Charakterizace a modifikace komplexní odpovědi buněk nádorů hlavy a krku na různá záření - krok kupředu ke kombinované personalizované (radio)terapii

Investor: Czech Science Foundation

Citovat

FALK, M., Zuzana HORÁKOVÁ, Markéta SVOBODOVÁ, Michal MASAŘÍK, O. KOPECNA, Jaromír GUMULEC, Martina RAUDENSKÁ, D. DEPES, A. BACIKOVA, I. FALKOVA and Hana BINKOVÁ. gamma H2AX/53BP1 foci as a potential pre-treatment marker of HNSCC tumors radiosensitivity - preliminary methodological study and discussion. The European Physical Journal D. NEW YORK: Springer Berlin Heidelberg, 2017, vol. 71, No 9, p. 1-7. ISSN 1434-6060. Available from: https://dx.doi.org/10.1140/epjd/e2017-80073-2.

@article{1391585,
   author = {Falk, M. and Horáková, Zuzana and Svobodová, Markéta and Masařík, Michal and Kopecna, O. and Gumulec, Jaromír and Raudenská, Martina and Depes, D. and Bacikova, A. and Falkova, I. and Binková, Hana},
   article_location = {NEW YORK},
   article_number = {9},
   doi = {http://dx.doi.org/10.1140/epjd/e2017-80073-2},
   keywords = {HNSCC tumor},
   language = {eng},
   issn = {1434-6060},
   journal = {The European Physical Journal D},
   title = {gamma H2AX/53BP1 foci as a potential pre-treatment marker of HNSCC tumors radiosensitivity - preliminary methodological study and discussion},
   volume = {71},
   year = {2017}
}

TY  - JOUR
ID  - 1391585
AU  - Falk, M. - Horáková, Zuzana - Svobodová, Markéta - Masařík, Michal - Kopecna, O. - Gumulec, Jaromír - Raudenská, Martina - Depes, D. - Bacikova, A. - Falkova, I. - Binková, Hana
PY  - 2017
TI  - gamma H2AX/53BP1 foci as a potential pre-treatment marker of HNSCC tumors radiosensitivity - preliminary methodological study and discussion
JF  - The European Physical Journal D
VL  - 71
IS  - 9
SP  - 1-7
EP  - 1-7
PB  - Springer Berlin Heidelberg
SN  - 14346060
KW  - HNSCC tumor
N2  - In order to improve patients' post-treatment quality of life, a shift from surgery to non-surgical (chemo) radio-treatment is recognized in head and neck oncology. However, about half of HNSCC tumors are resistant to irradiation and an efficient marker of individual tumor radiosensitivity is still missing. We analyzed whether various parameters of DNA double strand break (DSB) repair determined in vitro can predict, prior to clinical treatment initiation, the radiosensitivity of tumors. We compared formation and decrease of gamma H2AX/53BP1 foci in 48 h after irradiating tumor cell primocultures with 2 Gy of gamma-rays. To better understand complex tumor behavior, three different cell type primocultures - CD90(-), CD90(+), and a mixed culture of these cells - were isolated from 1 clinically radioresistant, 2 radiosensitive, and 4 undetermined HPV-HNSCC tumors and followed separately. While DSB repair was delayed and the number of persisting DSBs increased in the radiosensitive tumors, the results for the radioresistant tumor were similar to cultured normal human skin fibroblasts. Hence, DSB repair kinetics/efficiency may correlate with clinical response to radiotherapy for a subset of HNSCC tumors but the size (and therefore practical relevance) of this subset remains to be determined. The same is true for contribution of different cell type primocultures to tumor radioresistance.
ER  -

FALK, M., Zuzana HORÁKOVÁ, Markéta SVOBODOVÁ, Michal MASAŘÍK, O. KOPECNA, Jaromír GUMULEC, Martina RAUDENSKÁ, D. DEPES, A. BACIKOVA, I. FALKOVA and Hana BINKOVÁ. gamma H2AX/53BP1 foci as a potential pre-treatment marker of HNSCC tumors radiosensitivity - preliminary methodological study and discussion. \textit{The European Physical Journal D}. NEW YORK: Springer Berlin Heidelberg, 2017, vol.~71, No~9, p.~1-7. ISSN~1434-6060. Available from: https://dx.doi.org/10.1140/epjd/e2017-80073-2.

Detailed Information on Publication Record