Detailed Information on Publication Record
2017
Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma
FEDORKO, Michal, Jaroslav JURÁČEK, Michal STANÍK, Marek SVOBODA, Alexandr POPRACH et. al.Basic information
Original name
Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma
Name in Czech
Detekce let-7 miRNA v močovém supernatantu jako potenciální způsob diagnostiky nemetastatického karcinomu z renálních buněk
Authors
FEDORKO, Michal (703 Slovakia, belonging to the institution), Jaroslav JURÁČEK (203 Czech Republic, belonging to the institution), Michal STANÍK (703 Slovakia), Marek SVOBODA (203 Czech Republic), Alexandr POPRACH (203 Czech Republic), Tomáš BÜCHLER (203 Czech Republic), Dalibor PACÍK (203 Czech Republic, belonging to the institution), Jan DOLEŽEL (203 Czech Republic) and Ondřej SLABÝ (203 Czech Republic, guarantor, belonging to the institution)
Edition
Biochemia Medica, Zagreb, CROATIAN SOC MEDICAL BIOCHEMISTS, 2017, 1330-0962
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30200 3.2 Clinical medicine
Country of publisher
Croatia
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 3.653
RIV identification code
RIV/00216224:14110/17:00095662
Organization unit
Faculty of Medicine
UT WoS
000404505700018
Keywords (in Czech)
karcinom z renálních buněk; močové miRNA; let-7; diagnostický biomarker
Keywords in English
renal cell carcinoma; urinary miRNAs; let-7; diagnostic biomarker
Tags
International impact, Reviewed
Změněno: 21/3/2018 17:37, Soňa Böhmová
V originále
Introduction: Urinary microRNAs (miRNAs) are emerging as a clinically useful tool for early and non-invasive detection of various types of cancer. The aim of this study was to evaluate whether let-7 family miRNAs differ in their urinary concentrations between renal cell carcinoma (RCC) cases and healthy controls. Materials and methods: In the case-control study, 69 non-metastatic clear-cell RCC patients and 36 gender/age-matched healthy controls were prospectively enrolled. Total RNA was purified from cell-free supernatant of the 105 first morning urine specimens. Let-7 family miRNAs were determined in cell-free supernatant using quantitative miRNA real-time reverse-transcription PCR and absolute quantification approach. Results: Concentrations of all let-7 miRNAs (let-7a, let-7b, let-7c, let-7d, let-7e and let-7g) were significantly higher in urine samples obtained from RCC patients compared to healthy controls (P < 0.001; P < 0.001; P = 0.005; P = 0.006; P = 0.015 and P = 0.002, respectively). Subsequent ROC analysis has shown that let-7a concentration possesses good ability to differentiate between cases and controls with area under curve being 0.8307 (sensitivity 71%, specificity 81%). Conclusions: We have shown that let-7 miRNAs are abundant in the urine samples of patients with clear-cell RCC, and out of six let-7 family members, let-7a outperforms the others and presents promising non-invasive biomarker for the detection of RCC.
In Czech
MikroRNA v moči jsou potenciálním nástrojem pro časnou a neinvazivní detekci různých typů maligních nádorů. Cílem studie bylo zhodnotit, zda se liší koncentrace miRNA z rodiny let-7 v moči pacientů s renálním karcinomem od zdravých kontrol.
Links
| LQ1601, research and development project |
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| NV15-31071A, research and development project |
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| NV15-34678A, research and development project |
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