Other formats:
BibTeX
LaTeX
RIS
@article{1392343, author = {Asmari, Mufarreh and Michalcová, Lenka and Alhazmi, Hassan A. and Glatz, Zdeněk and El Deeb, Sami}, article_location = {Holandsko}, article_number = {137}, doi = {http://dx.doi.org/10.1016/j.microc.2017.10.004}, keywords = {Microscale thermophoresis; MS; biometals; deferiprone; CP20}, language = {eng}, issn = {0026-265X}, journal = {Microchemical Journal}, title = {Investigation of deferiprone binding to different essential metal ions using microscale thermophoresis and electrospray ionization mass spectrometry}, url = {http://dx.doi.org/10.1016/j.microc.2017.10.004}, volume = {2018}, year = {2018} }
TY - JOUR ID - 1392343 AU - Asmari, Mufarreh - Michalcová, Lenka - Alhazmi, Hassan A. - Glatz, Zdeněk - El Deeb, Sami PY - 2018 TI - Investigation of deferiprone binding to different essential metal ions using microscale thermophoresis and electrospray ionization mass spectrometry JF - Microchemical Journal VL - 2018 IS - 137 SP - 98-104 EP - 98-104 PB - Elsevier SN - 0026265X KW - Microscale thermophoresis KW - MS KW - biometals KW - deferiprone KW - CP20 UR - http://dx.doi.org/10.1016/j.microc.2017.10.004 N2 - In this study, the microscale thermophoresis (MST) method was applied to investigate the interaction of deferiprone with biometals (Fe3+, Cu2+, Zn2+, Co2+, Ni2+, Mn2+, Mg2+, and Ca2+). The MST results indicated significant interactions of deferiprone with Fe3+, Cu2+, Zn2+, Co2+, and Ni2+. The data fitted to the Hill model shows stoichiometry more than 1:1. No binding was observed for deferiprone with Mn2+, Mg2+, and Ca2+. Electrospray ionization mass spectrometry confirms the results obtained by MST indicating stable molecular ion peaks for the complexes of deferiprone with either Fe3+, Zn2+, Cu2+, Co2+, Ni2+, and Mn2+, but no binding was observed for deferiprone with either Ca2+ and Mg2+ under a gas phase state. MST shows a fast and simple approach to study the binding of deferiprone to different essential metal ions. Moreover, the complexes were stable for investigation by mass spectrometry under a soft ionization technique like used electrospray ionization, which aided to confirm binding stoichiometry. ER -
ASMARI, Mufarreh, Lenka MICHALCOVÁ, Hassan A. ALHAZMI, Zdeněk GLATZ and Sami EL DEEB. Investigation of deferiprone binding to different essential metal ions using microscale thermophoresis and electrospray ionization mass spectrometry. \textit{Microchemical Journal}. Holandsko: Elsevier, 2018, vol.~2018, No~137, p.~98-104. ISSN~0026-265X. Available from: https://dx.doi.org/10.1016/j.microc.2017.10.004.
|