Investigation of deferiprone binding to different essential
metal ions using microscale thermophoresis and electrospray
ionization mass spectrometry

J 2018

Investigation of deferiprone binding to different essential metal ions using microscale thermophoresis and electrospray ionization mass spectrometry

ASMARI, Mufarreh, Lenka MICHALCOVÁ, Hassan A. ALHAZMI, Zdeněk GLATZ, Sami EL DEEB et. al.

Basic information

Original name

Investigation of deferiprone binding to different essential metal ions using microscale thermophoresis and electrospray ionization mass spectrometry

Authors

ASMARI, Mufarreh (276 Germany), Lenka MICHALCOVÁ (203 Czech Republic, belonging to the institution), Hassan A. ALHAZMI (276 Germany), Zdeněk GLATZ (203 Czech Republic) and Sami EL DEEB (276 Germany, guarantor)

Edition

Microchemical Journal, Holandsko, Elsevier, 2018, 0026-265X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.206

RIV identification code

RIV/00216224:14310/18:00100730

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.microc.2017.10.004

UT WoS

000423894800015

Keywords (in Czech)

MST; MS; bio-kovy; deferiprone; CP20

Keywords in English

Microscale thermophoresis; MS; biometals; deferiprone; CP20

Abstract

V originále

In this study, the microscale thermophoresis (MST) method was applied to investigate the interaction of deferiprone with biometals (Fe3+, Cu2+, Zn2+, Co2+, Ni2+, Mn2+, Mg2+, and Ca2+). The MST results indicated significant interactions of deferiprone with Fe3+, Cu2+, Zn2+, Co2+, and Ni2+. The data fitted to the Hill model shows stoichiometry more than 1:1. No binding was observed for deferiprone with Mn2+, Mg2+, and Ca2+. Electrospray ionization mass spectrometry confirms the results obtained by MST indicating stable molecular ion peaks for the complexes of deferiprone with either Fe3+, Zn2+, Cu2+, Co2+, Ni2+, and Mn2+, but no binding was observed for deferiprone with either Ca2+ and Mg2+ under a gas phase state. MST shows a fast and simple approach to study the binding of deferiprone to different essential metal ions. Moreover, the complexes were stable for investigation by mass spectrometry under a soft ionization technique like used electrospray ionization, which aided to confirm binding stoichiometry.

Links

GBP206/12/G014, research and development project

Name: Centrum pokročilých bioanalytických technologií

MUNI/A/1278/2016, interní kód MU

Name: Podpora biochemického výzkumu v roce 2017

Investor: Masaryk University, Category A

Files attached

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Citovat

ASMARI, Mufarreh, Lenka MICHALCOVÁ, Hassan A. ALHAZMI, Zdeněk GLATZ and Sami EL DEEB. Investigation of deferiprone binding to different essential metal ions using microscale thermophoresis and electrospray ionization mass spectrometry. Microchemical Journal. Holandsko: Elsevier, 2018, vol. 2018, No 137, p. 98-104. ISSN 0026-265X. Available from: https://dx.doi.org/10.1016/j.microc.2017.10.004.

@article{1392343,
   author = {Asmari, Mufarreh and Michalcová, Lenka and Alhazmi, Hassan A. and Glatz, Zdeněk and El Deeb, Sami},
   article_location = {Holandsko},
   article_number = {137},
   doi = {http://dx.doi.org/10.1016/j.microc.2017.10.004},
   keywords = {Microscale thermophoresis; MS; biometals; deferiprone; CP20},
   language = {eng},
   issn = {0026-265X},
   journal = {Microchemical Journal},
   title = {Investigation of deferiprone binding to different essential metal ions using microscale thermophoresis and electrospray ionization mass spectrometry},
   url = {http://dx.doi.org/10.1016/j.microc.2017.10.004},
   volume = {2018},
   year = {2018}
}

TY  - JOUR
ID  - 1392343
AU  - Asmari, Mufarreh - Michalcová, Lenka - Alhazmi, Hassan A. - Glatz, Zdeněk - El Deeb, Sami
PY  - 2018
TI  - Investigation of deferiprone binding to different essential metal ions using microscale thermophoresis and electrospray ionization mass spectrometry
JF  - Microchemical Journal
VL  - 2018
IS  - 137
SP  - 98-104
EP  - 98-104
PB  - Elsevier
SN  - 0026265X
KW  - Microscale thermophoresis
KW  - MS
KW  - biometals
KW  - deferiprone
KW  - CP20
UR  - http://dx.doi.org/10.1016/j.microc.2017.10.004
N2  - In this study, the microscale thermophoresis (MST) method was applied to investigate the interaction of deferiprone with biometals (Fe3+, Cu2+, Zn2+, Co2+, Ni2+, Mn2+, Mg2+, and Ca2+). The MST results indicated significant interactions of deferiprone with Fe3+, Cu2+, Zn2+, Co2+, and Ni2+. The data fitted to the Hill model shows stoichiometry more than 1:1. No binding was observed for deferiprone with Mn2+, Mg2+, and Ca2+. Electrospray ionization mass spectrometry confirms the results obtained by MST indicating stable molecular ion peaks for the complexes of deferiprone with either Fe3+, Zn2+, Cu2+, Co2+, Ni2+, and Mn2+, but no binding was observed for deferiprone with either Ca2+ and Mg2+ under a gas phase state. MST shows a fast and simple approach to study the binding of deferiprone to different essential metal ions. Moreover, the complexes were stable for investigation by mass spectrometry under a soft ionization technique like used electrospray ionization, which aided to confirm binding stoichiometry.
ER  -

ASMARI, Mufarreh, Lenka MICHALCOVÁ, Hassan A. ALHAZMI, Zdeněk GLATZ and Sami EL DEEB. Investigation of deferiprone binding to different essential metal ions using microscale thermophoresis and electrospray ionization mass spectrometry. \textit{Microchemical Journal}. Holandsko: Elsevier, 2018, vol.~2018, No~137, p.~98-104. ISSN~0026-265X. Available from: https://dx.doi.org/10.1016/j.microc.2017.10.004.

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