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@article{1392343,
author = {Asmari, Mufarreh and Michalcová, Lenka and Alhazmi, Hassan A. and Glatz, Zdeněk and El Deeb, Sami},
article_location = {Holandsko},
article_number = {137},
doi = {http://dx.doi.org/10.1016/j.microc.2017.10.004},
keywords = {Microscale thermophoresis; MS; biometals; deferiprone; CP20},
language = {eng},
issn = {0026-265X},
journal = {Microchemical Journal},
title = {Investigation of deferiprone binding to different essential metal ions using microscale thermophoresis and electrospray ionization mass spectrometry},
url = {http://dx.doi.org/10.1016/j.microc.2017.10.004},
volume = {2018},
year = {2018}
}
TY - JOUR
ID - 1392343
AU - Asmari, Mufarreh - Michalcová, Lenka - Alhazmi, Hassan A. - Glatz, Zdeněk - El Deeb, Sami
PY - 2018
TI - Investigation of deferiprone binding to different essential metal ions using microscale thermophoresis and electrospray ionization mass spectrometry
JF - Microchemical Journal
VL - 2018
IS - 137
SP - 98-104
EP - 98-104
PB - Elsevier
SN - 0026265X
KW - Microscale thermophoresis
KW - MS
KW - biometals
KW - deferiprone
KW - CP20
UR - http://dx.doi.org/10.1016/j.microc.2017.10.004
N2 - In this study, the microscale thermophoresis (MST) method was applied to investigate the interaction of deferiprone with biometals (Fe3+, Cu2+, Zn2+, Co2+, Ni2+, Mn2+, Mg2+, and Ca2+). The MST results indicated significant interactions of deferiprone with Fe3+, Cu2+, Zn2+, Co2+, and Ni2+. The data fitted to the Hill model shows stoichiometry more than 1:1. No binding was observed for deferiprone with Mn2+, Mg2+, and Ca2+. Electrospray ionization mass spectrometry confirms the results obtained by MST indicating stable molecular ion peaks for the complexes of deferiprone with either Fe3+, Zn2+, Cu2+, Co2+, Ni2+, and Mn2+, but no binding was observed for deferiprone with either Ca2+ and Mg2+ under a gas phase state. MST shows a fast and simple approach to study the binding of deferiprone to different essential metal ions. Moreover, the complexes were stable for investigation by mass spectrometry under a soft ionization technique like used electrospray ionization, which aided to confirm binding stoichiometry.
ER -
ASMARI, Mufarreh, Lenka MICHALCOVÁ, Hassan A. ALHAZMI, Zdeněk GLATZ and Sami EL DEEB. Investigation of deferiprone binding to different essential metal ions using microscale thermophoresis and electrospray ionization mass spectrometry. \textit{Microchemical Journal}. Holandsko: Elsevier, 2018, vol.~2018, No~137, p.~98-104. ISSN~0026-265X. Available from: https://dx.doi.org/10.1016/j.microc.2017.10.004.
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