Caffeine administration alters the behaviour and development of
Galleria mellonella larvae

J 2017

Caffeine administration alters the behaviour and development of Galleria mellonella larvae

MAGUIRE, Ronan, Martin KUNC, Pavel HYRŠL and Kevin KAVANAGH

Basic information

Original name

Caffeine administration alters the behaviour and development of Galleria mellonella larvae

Name in Czech

Podávání kofeinu ovlivňuje chování a vývoj larev Galleria mellonella

Authors

MAGUIRE, Ronan (372 Ireland), Martin KUNC (203 Czech Republic, belonging to the institution), Pavel HYRŠL (203 Czech Republic, belonging to the institution) and Kevin KAVANAGH (372 Ireland, guarantor)

Edition

Neurotoxicology and Teratology, Oxford, PERGAMON-ELSEVIER SCIENCE LTD, 2017, 0892-0362

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.811

RIV identification code

RIV/00216224:14310/17:00097910

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.ntt.2017.10.002

UT WoS

000417669200005

Keywords (in Czech)

Kafein; Galleria; imunita; modelový organismus; in vivo screening

Keywords in English

Caffeine; Galleria; Immunity; Mini-model; in vivo screening

Abstract

V originále

The effect of feeding caffeine on the behaviour and neural proteome of Galleria mellonella larvae was assessed. Caffeine was administered to larvae by force feeding and the metabolites theobromine and theophylline were subsequently detected by RP-HPLC analysis. Administration of caffeine to larvae resulted in reduced movement and a reduction in the formation of pupae. The production of the muscle relaxant theophylline may contribute to the reduction in larval movement. Analysis of the changes in proteome of the brain and surrounding tissues of caffeine fed larvae revealed an increase in the abundance of immune related proteins such as immune-related Hdd1 (6.28 fold increase) and hemolin (1.68 fold increase), ATPase associated proteins such as H+ transporting ATP synthase O subunit isoform 1 (1.87 fold increase) and H+ transporting ATP synthase delta subunit (1.53 fold increase) and proteins indicative of brain trauma such as troponin T transcript variant B, partial (1.55 fold increase). Proteins involved in development and protein degradation such as SUMO-activating enzyme subunit 1 (3.08 fold decrease) and chitin deacetylase, partial (3.67 fold decrease) were decreased in abundance. The results presented here indicate that caffeine is metabolised in a similar way in G. mellonella larvae to that in mammals and results in a variety of behavioural and developmental alterations. Utilisation of insects for studying the effects of caffeine and other neuroactive compounds may offer new insights into their mode of action and reduce the need to use mammals for this type of analysis.

Citovat

MAGUIRE, Ronan, Martin KUNC, Pavel HYRŠL and Kevin KAVANAGH. Caffeine administration alters the behaviour and development of Galleria mellonella larvae. Neurotoxicology and Teratology. Oxford: PERGAMON-ELSEVIER SCIENCE LTD, 2017, vol. 64, November–December, p. 37-44. ISSN 0892-0362. Available from: https://dx.doi.org/10.1016/j.ntt.2017.10.002.

@article{1392362,
   author = {Maguire, Ronan and Kunc, Martin and Hyršl, Pavel and Kavanagh, Kevin},
   article_location = {Oxford},
   article_number = {November–December},
   doi = {http://dx.doi.org/10.1016/j.ntt.2017.10.002},
   keywords = {Caffeine; Galleria; Immunity; Mini-model; in vivo screening},
   language = {eng},
   issn = {0892-0362},
   journal = {Neurotoxicology and Teratology},
   title = {Caffeine administration alters the behaviour and development of Galleria mellonella larvae},
   url = {http://www.sciencedirect.com/science/article/pii/S0892036217301460?via%3Dihub},
   volume = {64},
   year = {2017}
}

TY  - JOUR
ID  - 1392362
AU  - Maguire, Ronan - Kunc, Martin - Hyršl, Pavel - Kavanagh, Kevin
PY  - 2017
TI  - Caffeine administration alters the behaviour and development of Galleria mellonella larvae
JF  - Neurotoxicology and Teratology
VL  - 64
IS  - November–December
SP  - 37-44
EP  - 37-44
PB  - PERGAMON-ELSEVIER SCIENCE LTD
SN  - 08920362
KW  - Caffeine
KW  - Galleria
KW  - Immunity
KW  - Mini-model
KW  - in vivo screening
UR  - http://www.sciencedirect.com/science/article/pii/S0892036217301460?via%3Dihub
L2  - http://www.sciencedirect.com/science/article/pii/S0892036217301460?via%3Dihub
N2  - The effect of feeding caffeine on the behaviour and neural proteome of Galleria mellonella larvae was assessed. Caffeine was administered to larvae by force feeding and the metabolites theobromine and theophylline were subsequently detected by RP-HPLC analysis. Administration of caffeine to larvae resulted in reduced movement and a reduction in the formation of pupae. The production of the muscle relaxant theophylline may contribute to the reduction in larval movement. Analysis of the changes in proteome of the brain and surrounding tissues of caffeine fed larvae revealed an increase in the abundance of immune related proteins such as immune-related Hdd1 (6.28 fold increase) and hemolin (1.68 fold increase), ATPase associated proteins such as H+ transporting ATP synthase O subunit isoform 1 (1.87 fold increase) and H+ transporting ATP synthase delta subunit (1.53 fold increase) and proteins indicative of brain trauma such as troponin T transcript variant B, partial (1.55 fold increase). Proteins involved in development and protein degradation such as SUMO-activating enzyme subunit 1 (3.08 fold decrease) and chitin deacetylase, partial (3.67 fold decrease) were decreased in abundance. The results presented here indicate that caffeine is metabolised in a similar way in G. mellonella larvae to that in mammals and results in a variety of behavioural and developmental alterations. Utilisation of insects for studying the effects of caffeine and other neuroactive compounds may offer new insights into their mode of action and reduce the need to use mammals for this type of analysis.
ER  -

MAGUIRE, Ronan, Martin KUNC, Pavel HYRŠL and Kevin KAVANAGH. Caffeine administration alters the behaviour and development of Galleria mellonella larvae. \textit{Neurotoxicology and Teratology}. Oxford: PERGAMON-ELSEVIER SCIENCE LTD, 2017, vol.~64, November–December, p.~37-44. ISSN~0892-0362. Available from: https://dx.doi.org/10.1016/j.ntt.2017.10.002.

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