MAGUIRE, Ronan, Martin KUNC, Pavel HYRŠL and Kevin KAVANAGH. Caffeine administration alters the behaviour and development of Galleria mellonella larvae. Neurotoxicology and Teratology. Oxford: PERGAMON-ELSEVIER SCIENCE LTD, vol. 64, November–December, p. 37-44. ISSN 0892-0362. doi:10.1016/j.ntt.2017.10.002. 2017.
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Basic information
Original name Caffeine administration alters the behaviour and development of Galleria mellonella larvae
Name in Czech Podávání kofeinu ovlivňuje chování a vývoj larev Galleria mellonella
Authors MAGUIRE, Ronan (372 Ireland), Martin KUNC (203 Czech Republic, belonging to the institution), Pavel HYRŠL (203 Czech Republic, belonging to the institution) and Kevin KAVANAGH (372 Ireland, guarantor).
Edition Neurotoxicology and Teratology, Oxford, PERGAMON-ELSEVIER SCIENCE LTD, 2017, 0892-0362.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30105 Physiology
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 2.811
RIV identification code RIV/00216224:14310/17:00097910
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1016/j.ntt.2017.10.002
UT WoS 000417669200005
Keywords (in Czech) Kafein; Galleria; imunita; modelový organismus; in vivo screening
Keywords in English Caffeine; Galleria; Immunity; Mini-model; in vivo screening
Tags NZ, rivok
Tags International impact, Reviewed
Changed by Changed by: Ing. Nicole Zrilić, učo 240776. Changed: 29/3/2018 15:24.
Abstract
The effect of feeding caffeine on the behaviour and neural proteome of Galleria mellonella larvae was assessed. Caffeine was administered to larvae by force feeding and the metabolites theobromine and theophylline were subsequently detected by RP-HPLC analysis. Administration of caffeine to larvae resulted in reduced movement and a reduction in the formation of pupae. The production of the muscle relaxant theophylline may contribute to the reduction in larval movement. Analysis of the changes in proteome of the brain and surrounding tissues of caffeine fed larvae revealed an increase in the abundance of immune related proteins such as immune-related Hdd1 (6.28 fold increase) and hemolin (1.68 fold increase), ATPase associated proteins such as H+ transporting ATP synthase O subunit isoform 1 (1.87 fold increase) and H+ transporting ATP synthase delta subunit (1.53 fold increase) and proteins indicative of brain trauma such as troponin T transcript variant B, partial (1.55 fold increase). Proteins involved in development and protein degradation such as SUMO-activating enzyme subunit 1 (3.08 fold decrease) and chitin deacetylase, partial (3.67 fold decrease) were decreased in abundance. The results presented here indicate that caffeine is metabolised in a similar way in G. mellonella larvae to that in mammals and results in a variety of behavioural and developmental alterations. Utilisation of insects for studying the effects of caffeine and other neuroactive compounds may offer new insights into their mode of action and reduce the need to use mammals for this type of analysis.
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