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@article{1392777,
author = {Celá, Andrea and Mádr, Aleš and Glatz, Zdeněk},
article_location = {Amsterdam, Netherlands},
article_number = {May},
doi = {http://dx.doi.org/10.1016/j.chroma.2017.03.080},
keywords = {Capillary electrophoresis; Electrophoretically mediated microanalysis; Amino acids; On-capillary derivatization; Human embryo metabolomics; Transverse diffusion of laminar flow profiles},
language = {eng},
issn = {0021-9673},
journal = {Journal of Chromatography A},
title = {Electrophoretically mediated microanalysis for simultaneous on-capillary derivatization of standard amino acids followed by micellar electrokinetic capillary chromatography with laser-induced fluorescence detection},
volume = {1499},
year = {2017}
}
TY - JOUR
ID - 1392777
AU - Celá, Andrea - Mádr, Aleš - Glatz, Zdeněk
PY - 2017
TI - Electrophoretically mediated microanalysis for simultaneous on-capillary derivatization of standard amino acids followed by micellar electrokinetic capillary chromatography with laser-induced fluorescence detection
JF - Journal of Chromatography A
VL - 1499
IS - May
SP - 203-210
EP - 203-210
PB - Elsevier Science BV
SN - 00219673
KW - Capillary electrophoresis
KW - Electrophoretically mediated microanalysis
KW - Amino acids
KW - On-capillary derivatization
KW - Human embryo metabolomics
KW - Transverse diffusion of laminar flow profiles
N2 - Amino acids are crucial compounds involved in most biochemical processes essential for life. Since their dynamic turnover reflects the actual physiology of the cell/organism, a turnover assessment may provide valuable information related to multiple physiological and pathophysiological conditions. The sensitive determination of amino acids is predominantly associated with their derivatization which might be laborious, time-consuming and difficult to standardize. However, capillary electrophoresis offers the automatic injection and mixing of reactants, incubation of the reaction mixture, separation and detection of the reaction products in one on-capillary procedure. Among the on-capillary mixing strategies, electrophoretically mediated microanalysis (EMMA) is superior in terms of mixing efficiency. In this paper, we present an optimization of EMMA for the simultaneous derivatization of standard amino acids by naphthalene-2,3-dicarboxaldehyde/NaCN and its application to targeted human embryo metabolomics. For such a purpose, novel separation conditions were developed involving the background electrolyte, comprised of 73 mM sodium dodecyl sulfate, 6.7 % (v/v) 1-propanol, 0.5 mM (2-hydroxypropyl)-beta-cyclodextrin and 135 mM boric acid/sodium hydroxide buffer (pH 9.00). Finally, the optimized EMMA was compared to a fundamentally different mixing strategy, namely the transverse diffusion of laminar flow profiles, and proved to be also suitable for human plasma analysis.
ER -
CELÁ, Andrea, Aleš MÁDR and Zdeněk GLATZ. Electrophoretically mediated microanalysis for simultaneous on-capillary derivatization of standard amino acids followed by micellar electrokinetic capillary chromatography with laser-induced fluorescence detection. \textit{Journal of Chromatography A}. Amsterdam, Netherlands: Elsevier Science BV, 2017, vol.~1499, May, p.~203-210. ISSN~0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2017.03.080.
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