HDAC1 and HDAC3 underlie dynamic H3K9 acetylation during
embryonic neurogenesis and in schizophrenia-like animals

J 2018

HDAC1 and HDAC3 underlie dynamic H3K9 acetylation during embryonic neurogenesis and in schizophrenia-like animals

VEČEŘA, Josef, Eva BÁRTOVÁ, Jana KREJČÍ, Soňa LEGARTOVÁ, Denisa KOMŮRKOVÁ et. al.

Basic information

Original name

HDAC1 and HDAC3 underlie dynamic H3K9 acetylation during embryonic neurogenesis and in schizophrenia-like animals

Authors

VEČEŘA, Josef (203 Czech Republic, belonging to the institution), Eva BÁRTOVÁ (203 Czech Republic, guarantor), Jana KREJČÍ (203 Czech Republic), Soňa LEGARTOVÁ (703 Slovakia), Denisa KOMŮRKOVÁ (203 Czech Republic), Jana RUDÁ (203 Czech Republic, belonging to the institution), Tibor ŠTARK (703 Slovakia, belonging to the institution), Eva DRAŽANOVÁ (203 Czech Republic, belonging to the institution), Tomáš KAŠPÁREK (203 Czech Republic, belonging to the institution), Alexandra ŠULCOVÁ (203 Czech Republic, belonging to the institution), Frank J. DEKKER (528 Netherlands), Wiktor SZYMANSKI (528 Netherlands), Christian SEISER (40 Austria), Georg WEITZER (40 Austria), Raphael MECHOULAM (376 Israel), Vincenzo MICALE (380 Italy, belonging to the institution) and Stanislav KOZUBEK (203 Czech Republic, belonging to the institution)

Edition

Journal of cellular physiology, United States, Wiley-Liss, 2018, 0021-9541

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.522

RIV identification code

RIV/00216224:14110/18:00100736

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1002/jcp.25914

UT WoS

000411829600046

Keywords in English

acetylome; H3K9 acetylation; HDACs; mouse neurogenesis; schizophrenia

Abstract

V originále

Although histone acetylation is one of the most widely studied epigenetic modifications, there is still a lack of information regarding how the acetylome is regulated during brain development and pathophysiological processes. We demonstrate that the embryonic brain (E15) is characterized by an increase in H3K9 acetylation as well as decreases in the levels of HDAC1 and HDAC3. Moreover, experimental induction of H3K9 hyperacetylation led to the overexpression of NCAM in the embryonic cortex and depletion of Sox2 in the subventricular ependyma, which mimicked the differentiation processes. Inducing differentiation in HDAC1-deficient mouse ESCs resulted in early H3K9 deacetylation, Sox2 downregulation, and enhanced astrogliogenesis, whereas neuro-differentiation was almost suppressed. Neuro-differentiation of (wt) ESCs was characterized by H3K9 hyperacetylation that was associated with HDAC1 and HDAC3 depletion. Conversely, the hippocampi of schizophrenia-like animals showed H3K9 deacetylation that was regulated by an increase in both HDAC1 and HDAC3. The hippocampi of schizophrenia-like brains that were treated with the cannabinoid receptor-1 inverse antagonist AM251 expressed H3K9ac at the level observed in normal brains. Together, the results indicate that co-regulation of H3K9ac by HDAC1 and HDAC3 is important to both embryonic brain development and neuro-differentiation as well as the pathophysiology of a schizophrenia-like phenotype.

Links

GBP302/12/G157, research and development project

Name: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Czech Science Foundation

GJ15-13443Y, research and development project

Name: Úloha hypoxií indukovaného faktoru 1 alfa ve vývoji populace neurálních kmenových buněk myši

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

3SGA5789, interní kód MU

Name: PRECIPITATION OF SCHIZOPHRENIA-LIKE PHENOTYPE BY PRENATAL INFLUENCES: ASSESSING THE ROLE OF THE ENDOCANNABINOID SYSTEM (Acronym: ncRNAPain)

Investor: South-Moravian Region, Incoming grants

Citovat

VEČEŘA, Josef, Eva BÁRTOVÁ, Jana KREJČÍ, Soňa LEGARTOVÁ, Denisa KOMŮRKOVÁ, Jana RUDÁ, Tibor ŠTARK, Eva DRAŽANOVÁ, Tomáš KAŠPÁREK, Alexandra ŠULCOVÁ, Frank J. DEKKER, Wiktor SZYMANSKI, Christian SEISER, Georg WEITZER, Raphael MECHOULAM, Vincenzo MICALE and Stanislav KOZUBEK. HDAC1 and HDAC3 underlie dynamic H3K9 acetylation during embryonic neurogenesis and in schizophrenia-like animals. Journal of cellular physiology. United States: Wiley-Liss, 2018, vol. 233, No 1, p. 530-548. ISSN 0021-9541. Available from: https://dx.doi.org/10.1002/jcp.25914.

@article{1393528,
   author = {Večeřa, Josef and Bártová, Eva and Krejčí, Jana and Legartová, Soňa and Komůrková, Denisa and Rudá, Jana and Štark, Tibor and Dražanová, Eva and Kašpárek, Tomáš and Šulcová, Alexandra and Dekker, Frank J. and Szymanski, Wiktor and Seiser, Christian and Weitzer, Georg and Mechoulam, Raphael and Micale, Vincenzo and Kozubek, Stanislav},
   article_location = {United States},
   article_number = {1},
   doi = {http://dx.doi.org/10.1002/jcp.25914},
   keywords = {acetylome; H3K9 acetylation; HDACs; mouse neurogenesis; schizophrenia},
   language = {eng},
   issn = {0021-9541},
   journal = {Journal of cellular physiology},
   title = {HDAC1 and HDAC3 underlie dynamic H3K9 acetylation during embryonic neurogenesis and in schizophrenia-like animals},
   url = {http://dx.doi.org/10.1002/jcp.25914},
   volume = {233},
   year = {2018}
}

TY  - JOUR
ID  - 1393528
AU  - Večeřa, Josef - Bártová, Eva - Krejčí, Jana - Legartová, Soňa - Komůrková, Denisa - Rudá, Jana - Štark, Tibor - Dražanová, Eva - Kašpárek, Tomáš - Šulcová, Alexandra - Dekker, Frank J. - Szymanski, Wiktor - Seiser, Christian - Weitzer, Georg - Mechoulam, Raphael - Micale, Vincenzo - Kozubek, Stanislav
PY  - 2018
TI  - HDAC1 and HDAC3 underlie dynamic H3K9 acetylation during embryonic neurogenesis and in schizophrenia-like animals
JF  - Journal of cellular physiology
VL  - 233
IS  - 1
SP  - 530-548
EP  - 530-548
PB  - Wiley-Liss
SN  - 00219541
KW  - acetylome
KW  - H3K9 acetylation
KW  - HDACs
KW  - mouse neurogenesis
KW  - schizophrenia
UR  - http://dx.doi.org/10.1002/jcp.25914
N2  - Although histone acetylation is one of the most widely studied epigenetic modifications, there is still a lack of information regarding how the acetylome is regulated during brain development and pathophysiological processes. We demonstrate that the embryonic brain (E15) is characterized by an increase in H3K9 acetylation as well as decreases in the levels of HDAC1 and HDAC3. Moreover, experimental induction of H3K9 hyperacetylation led to the overexpression of NCAM in the embryonic cortex and depletion of Sox2 in the subventricular ependyma, which mimicked the differentiation processes. Inducing differentiation in HDAC1-deficient mouse ESCs resulted in early H3K9 deacetylation, Sox2 downregulation, and enhanced astrogliogenesis, whereas neuro-differentiation was almost suppressed. Neuro-differentiation of (wt) ESCs was characterized by H3K9 hyperacetylation that was associated with HDAC1 and HDAC3 depletion. Conversely, the hippocampi of schizophrenia-like animals showed H3K9 deacetylation that was regulated by an increase in both HDAC1 and HDAC3. The hippocampi of schizophrenia-like brains that were treated with the cannabinoid receptor-1 inverse antagonist AM251 expressed H3K9ac at the level observed in normal brains. Together, the results indicate that co-regulation of H3K9ac by HDAC1 and HDAC3 is important to both embryonic brain development and neuro-differentiation as well as the pathophysiology of a schizophrenia-like phenotype.
ER  -

VEČEŘA, Josef, Eva BÁRTOVÁ, Jana KREJČÍ, Soňa LEGARTOVÁ, Denisa KOMŮRKOVÁ, Jana RUDÁ, Tibor ŠTARK, Eva DRAŽANOVÁ, Tomáš KAŠPÁREK, Alexandra ŠULCOVÁ, Frank J. DEKKER, Wiktor SZYMANSKI, Christian SEISER, Georg WEITZER, Raphael MECHOULAM, Vincenzo MICALE and Stanislav KOZUBEK. HDAC1 and HDAC3 underlie dynamic H3K9 acetylation during embryonic neurogenesis and in schizophrenia-like animals. \textit{Journal of cellular physiology}. United States: Wiley-Liss, 2018, vol.~233, No~1, p.~530-548. ISSN~0021-9541. Available from: https://dx.doi.org/10.1002/jcp.25914.

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