BELICKY, Stefan, Hana CERNOCKA, Tomas BERTOK, Alena HOLAZOVA, Kamila RÉBLOVÁ, Emil PALECEK, Jan TKAC and Veronika OSTATNA. Label-free chronopotentiometric glycoprofiling of prostate specific antigen using sialic acid recognizing lectins. Bioelectrochemistry. SWITZERLAND: Elsevier Science SA, 2017, vol. 117, October, p. 89-94. ISSN 1567-5394. Available from: https://dx.doi.org/10.1016/j.bioelechem.2017.06.005. |
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@article{1394044, author = {Belicky, Stefan and Cernocka, Hana and Bertok, Tomas and Holazova, Alena and Réblová, Kamila and Palecek, Emil and Tkac, Jan and Ostatna, Veronika}, article_location = {SWITZERLAND}, article_number = {October}, doi = {http://dx.doi.org/10.1016/j.bioelechem.2017.06.005}, keywords = {A prostate specific antigen; Lectin-glycoprotein interaction; Sialylated glycan isomers; Chronopotentiometric analysis; Mercury electrode}, language = {eng}, issn = {1567-5394}, journal = {Bioelectrochemistry}, title = {Label-free chronopotentiometric glycoprofiling of prostate specific antigen using sialic acid recognizing lectins}, url = {http://www.sciencedirect.com/science/article/pii/S1567539417301019?via%3Dihub}, volume = {117}, year = {2017} }
TY - JOUR ID - 1394044 AU - Belicky, Stefan - Cernocka, Hana - Bertok, Tomas - Holazova, Alena - Réblová, Kamila - Palecek, Emil - Tkac, Jan - Ostatna, Veronika PY - 2017 TI - Label-free chronopotentiometric glycoprofiling of prostate specific antigen using sialic acid recognizing lectins JF - Bioelectrochemistry VL - 117 IS - October SP - 89-94 EP - 89-94 PB - Elsevier Science SA SN - 15675394 KW - A prostate specific antigen KW - Lectin-glycoprotein interaction KW - Sialylated glycan isomers KW - Chronopotentiometric analysis KW - Mercury electrode UR - http://www.sciencedirect.com/science/article/pii/S1567539417301019?via%3Dihub L2 - http://www.sciencedirect.com/science/article/pii/S1567539417301019?via%3Dihub N2 - In recent decades, it has become clear that most of human proteins are glycosylated and that protein glycosylation plays an important role in health and diseases. At present, simple, fast and inexpensive methods are sought for clinical applications and particularly for improved diagnostics of various diseases, including cancer. We propose a label- and reagent-free electrochemical method based on chronopotentiometric stripping (CPS) analysis and a hanging mercury drop electrode for the detection of interaction of sialylated protein biomarker a prostate specific antigen (PSA) with two important lectins: Sambucus nigra agglutinin (SNA) and Maackia amurensis agglutinin (MAA). Incubation of PSA-modified electrode with specific SNA lectin resulted in an increase of CPS peak H of the complex as compared to this peak of individual PSA. By adjusting polarization current and temperature, PSA-MAA interaction can be either eliminated or distinguished from the more abundant PSA-SNA complex. CPS data were in a good agreement with the data obtained by complementary methods, namely surface plasmon resonance and fluorescent lectin microarray. It can be anticipated that CPS will find application in glycomics and proteomics. (C) 2017 Elsevier B.V. All tights reserved. ER -
BELICKY, Stefan, Hana CERNOCKA, Tomas BERTOK, Alena HOLAZOVA, Kamila RÉBLOVÁ, Emil PALECEK, Jan TKAC and Veronika OSTATNA. Label-free chronopotentiometric glycoprofiling of prostate specific antigen using sialic acid recognizing lectins. \textit{Bioelectrochemistry}. SWITZERLAND: Elsevier Science SA, 2017, vol.~117, October, p.~89-94. ISSN~1567-5394. Available from: https://dx.doi.org/10.1016/j.bioelechem.2017.06.005.
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