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@article{1394260,
author = {Garlíková, Zuzana and Silva, Ana Catarina and Rabata, Anas and Potěšil, David and Ihnatová, Ivana and Dumková, Jana and Koledová, Zuzana and Zdráhal, Zbyněk and Vinarský, Vladimír and Hampl, Aleš and PintoanddoandÓ, Perpétua and Nascimento, Diana Santos},
article_location = {New Rochelle},
article_number = {1},
doi = {http://dx.doi.org/10.1089/ten.tec.2017.0283},
keywords = {decellularization; lung; extracellular matrix; in vitro models; lung fibroblasts; biological scaffold},
language = {eng},
issn = {1937-3384},
journal = {TISSUE ENGINEERING PART C-METHODS},
title = {Generation of a Close-to-Native In Vitro System to Study Lung Cells-Extracellular Matrix Crosstalk},
url = {http://online.liebertpub.com/doi/abs/10.1089/ten.tec.2017.0283?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed},
volume = {24},
year = {2018}
}
TY - JOUR
ID - 1394260
AU - Garlíková, Zuzana - Silva, Ana Catarina - Rabata, Anas - Potěšil, David - Ihnatová, Ivana - Dumková, Jana - Koledová, Zuzana - Zdráhal, Zbyněk - Vinarský, Vladimír - Hampl, Aleš - Pinto-do-Ó, Perpétua - Nascimento, Diana Santos
PY - 2018
TI - Generation of a Close-to-Native In Vitro System to Study Lung Cells-Extracellular Matrix Crosstalk
JF - TISSUE ENGINEERING PART C-METHODS
VL - 24
IS - 1
SP - 1-13
EP - 1-13
PB - Mary Ann Liebert
SN - 19373384
KW - decellularization
KW - lung
KW - extracellular matrix
KW - in vitro models
KW - lung fibroblasts
KW - biological scaffold
UR - http://online.liebertpub.com/doi/abs/10.1089/ten.tec.2017.0283?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed
N2 - Extracellular matrix (ECM) is an essential component of the tissue microenvironment, actively shaping cellular behavior. In vitro culture systems are often poor in ECM constituents, thus not allowing for naturally occurring cell–ECM interactions. This study reports on a straightforward and efficient method for the generation of ECM scaffolds from lung tissue and its subsequent in vitro application using primary lung cells. Mouse lung tissue was subjected to decellularization with 0.2% sodium dodecyl sulfate, hypotonic solutions, and DNase. Resultant ECM scaffolds were devoid of cells and DNA, whereas lung ECM architecture of alveolar region and blood and airway networks were preserved. Scaffolds were predominantly composed of core ECM and ECM-associated proteins such as collagens I-IV, nephronectin, heparan sulfate proteoglycan core protein, and lysyl oxidase homolog 1, among others. When homogenized and applied as coating substrate, ECM supported the attachment of lung fibroblasts (LFs) in a dose-dependent manner. After ECM characterization and biocompatibility tests, a novel in vitro platform for three-dimensional (3D) matrix repopulation that permits live imaging of cell–ECM interactions was established. Using this system, LFs colonized the ECM scaffolds, displaying a close-to-native morphology in intimate interaction with the ECM fibers, and showed nuclear translocation of the mechanosensor yes-associated protein (YAP), when compared with cells cultured in two dimensions. In conclusion, we developed a 3D-like culture system, by combining an efficient decellularization method with a live-imaging culture platform, to replicate in vitro native lung cell–ECM crosstalk. This is a valuable system that can be easily applied to other organs for ECM-related drug screening, disease modeling, and basic mechanistic studies.
ER -
GARLÍKOVÁ, Zuzana, Ana Catarina SILVA, Anas RABATA, David POTĚŠIL, Ivana IHNATOVÁ, Jana DUMKOVÁ, Zuzana KOLEDOVÁ, Zbyněk ZDRÁHAL, Vladimír VINARSKÝ, Aleš HAMPL, Perpétua PINTO-DO-Ó and Diana Santos NASCIMENTO. Generation of a Close-to-Native In Vitro System to Study Lung Cells-Extracellular Matrix Crosstalk. \textit{TISSUE ENGINEERING PART C-METHODS}. New Rochelle: Mary Ann Liebert, 2018, vol.~24, No~1, p.~1-13. ISSN~1937-3384. Available from: https://dx.doi.org/10.1089/ten.tec.2017.0283.
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